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Sensors 2018, 18(12), 4326; https://doi.org/10.3390/s18124326

Fluorescent Aptamer Immobilization on Inverse Colloidal Crystals

1
CNR-IFN CSMFO Lab. and FBK CMM, via alla Cascata 56/C, 38123 Povo Trento, Italy
2
FBK-LaBSSAH, via Sommarive 18, 38123 Povo Trento, Italy
3
Indivenire Srl, via Alla Cascata 56/C, 38123 Povo Trento, Italy
4
CNR-IFN Milano, piazza Leonardo da Vinci 32, 20133 Milano, Italy
5
Physics Department, University of Trento, via Sommarive 14, 38123 Trento, Italy
6
Institute of Biophysics, CNR, via Sommarive 18, 38123 Povo Trento, Italy
7
IFAC—CNR, MiPLab., via Madonna del Piano10, 50019 Sesto Fiorentino, Italy
8
Enrico Fermi Centre, piazza del Viminale 1, 00184 Roma, Italy
*
Author to whom correspondence should be addressed.
Received: 10 October 2018 / Revised: 3 December 2018 / Accepted: 4 December 2018 / Published: 7 December 2018
(This article belongs to the Special Issue Resonator Sensors 2018)
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Abstract

In this paper, we described a versatile two steps approach for the realization of silica inverse opals functionalized with DNA-aptamers labelled with Cy3 fluorophore. The co-assembly method was successfully employed for the realization of high quality inverse silica opal, whilst the inverse network was functionalized via epoxy chemistry. Morphological and optical assessment revealed the presence of large ordered domains with a transmission band gap depth of 32%, after the functionalization procedure. Finite Difference Time-Domain (FDTD) simulations confirmed the high optical quality of the inverse opal realized. Photoluminescence measurements evidenced the effective immobilization of DNA-aptamer molecules labelled with Cy3 throughout the entire sample thickness. This assumption was verified by the inhibition of the fluorescence of Cy3 fluorophore tailoring the position of the photonic band gap of the inverse opal. The modification of the fluorescence could be justified by a variation in the density of states (DOS) calculated by the Plane Wave Expansion (PWE) method. Finally, the development of the aforementioned approach could be seen as proof of the concept experiment, suggesting that this type of system may act as a suitable platform for the realization of fluorescence-based bio-sensors. View Full-Text
Keywords: colloidal crystal; fluorescence; band gap; co-assembly; DNA-aptamers; FDTD simulations; PWE method colloidal crystal; fluorescence; band gap; co-assembly; DNA-aptamers; FDTD simulations; PWE method
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Chiappini, A.; Pasquardini, L.; Nodehi, S.; Armellini, C.; Bazzanella, N.; Lunelli, L.; Pelli, S.; Ferrari, M.; Pietralunga, S.M. Fluorescent Aptamer Immobilization on Inverse Colloidal Crystals. Sensors 2018, 18, 4326.

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