Next Article in Journal
Electroanalytical Determination of Cysteine Using the Electrodes Based on Ternary Silver-Copper Sulfides
Previous Article in Journal
A Design and Simulation of the Opportunistic Computation Offloading with Learning-Based Prediction for Unmanned Aerial Vehicle (UAV) Clustering Networks
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle

Amphiphilic Fluorine-Containing Block Copolymers as Carriers for Hydrophobic PtTFPP for Dissolved Oxygen Sensing, Cell Respiration Monitoring and In Vivo Hypoxia Imaging with High Quantum Efficiency and Long Lifetime

1
School of Materials Science and Engineering, Harbin Institute of Technology, Nangang District, Harbin 150001, China
2
Department of Materials Science and Engineering, Southern University of Science and Technology, Xili, Nanshan District, Shenzhen 518055, China
3
Light Chemical Technology College, Guangdong Industry Polytechnic, Haizhu District, Guangzhou 510300, China
4
SUSTech Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, Xili, Nanshan District, Shenzhen 518055, China
*
Authors to whom correspondence should be addressed.
Sensors 2018, 18(11), 3752; https://doi.org/10.3390/s18113752
Received: 3 October 2018 / Revised: 27 October 2018 / Accepted: 29 October 2018 / Published: 2 November 2018
(This article belongs to the Section Chemical Sensors)
  |  
PDF [3652 KB, uploaded 7 November 2018]
  |  

Abstract

New amphiphilic star or multi-arm block copolymers with different structures were synthesized for enabling the use of hydrophobic oxygen probe of platinum (II)-tetrakis (pentafluorophenyl) porphyrin (PtTFPP) for bioanalysis. The amphiphilic star polymers were prepared through the Atom Transfer Radical Polymerization (ATRP) method by using hydrophilic 4-arm polyethylene glycol (4-arm-PEG) as an initiator. Among the five block copolymers, P1 series (P1a, P1b, and P1c) and P3 possess fluorine-containing moieties to improve the oxygen sensitivity with its excellent capacity to dissolve and carry oxygen. A polymer P2 without fluorine units was also synthesized for comparison. The structure-property relationship was investigated. Under nitrogen atmosphere, high quantum efficiency of PtTFPP in fluorine-containing micelles could reach to 22% and long lifetime could reach to 76 μs. One kind of representative PtTFPP-containing micelles was used to detect the respiration of Escherichia coli (E. coli) JM109 and macrophage cell J774A.1 by a high throughput plate reader. In vivo hypoxic imaging of tumor-bearing mice was also achieved successfully. This study demonstrated that using well-designed fluoropolymers to load PtTFPP could achieve high oxygen sensing properties, and long lifetime, showing the great capability for further in vivo sensing and imaging. View Full-Text
Keywords: dissolved oxygen sensors; fluoropolymers; micelles; cell respiration monitoring; tumor imaging dissolved oxygen sensors; fluoropolymers; micelles; cell respiration monitoring; tumor imaging
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Li, J.; Qiao, Y.; Pan, T.; Zhong, K.; Wen, J.; Wu, S.; Su, F.; Tian, Y. Amphiphilic Fluorine-Containing Block Copolymers as Carriers for Hydrophobic PtTFPP for Dissolved Oxygen Sensing, Cell Respiration Monitoring and In Vivo Hypoxia Imaging with High Quantum Efficiency and Long Lifetime. Sensors 2018, 18, 3752.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top