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Sensors 2018, 18(1), 23; https://doi.org/10.3390/s18010023

Epigallocatechin Gallate-Modified Graphite Paste Electrode for Simultaneous Detection of Redox-Active Biomolecules

1
Department of Physical and Environmental Sciences, University of Toronto, Scarborough 1265 Military Trail, Toronto, ON M1C 1A4, Canada
2
Analytical Research Laboratory, Department of Chemistry, University of Sistan and Baluchestan, P.O. Box 98135-674, Zahedan, Iran
*
Author to whom correspondence should be addressed.
Received: 13 October 2017 / Revised: 7 December 2017 / Accepted: 12 December 2017 / Published: 22 December 2017
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Abstract

In this study, simultaneous electrochemical detection of ascorbic acid (AA), dopamine (DA), and uric acid (UA) was performed using a modified graphite paste electrode (MGPE) with epigallocatechin gallate (EGCG) and green tea (GT) powder. It was shown that the anodic peak current increased in comparison with that of the graphite paste electrode (GPE) in the cyclic voltammograms. The optimal pH for simultaneous determination of a quaternary mixture of AA–DA–UA was determined to be pH 2. The anodic peak potentials for a mixture containing AA–DA–UA were well separated from each other. The catalytic peak currents obtained at the surface of the MGPE/EGCG were linearly dependent on the AA, DA, and UA concentrations up to 23, 14, and 14 µM, respectively. The detection limits for AA, DA, and UA were 190, 90, and 70 nM, respectively. The analytical performance of this sensor has been evaluated for simultaneous detection of AA, DA, and UA in real samples. Finally, a modified electrode was prepared using GT and used for simultaneous determination of AA, DA, and UA. Based on the results, MPGE/GT showed two oxidation peaks at 0.43 and 0.6 V for DA and UA, respectively, without any oxidation peak for AA. The calibration curves at the surface of MGPE/GT were linear up to 14 µM with a detection limit of 0.18 and 0.33 µM for DA and UA, respectively. MGPEs provide a promising platform for the future development of sensors for multiplexed electrochemical detection of clinically important analytes. View Full-Text
Keywords: modified graphite paste electrode; epigallocatechin gallate; green tea; simultaneous determination; real samples modified graphite paste electrode; epigallocatechin gallate; green tea; simultaneous determination; real samples
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ganesh, H.V.S.; Noroozifar, M.; Kerman, K. Epigallocatechin Gallate-Modified Graphite Paste Electrode for Simultaneous Detection of Redox-Active Biomolecules. Sensors 2018, 18, 23.

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