Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method
AbstractA simple method of nanoparticle decoration can be used in the detection of pneumococcus. After the pneumococcal bacteria were captured by an antibody (pneumococcal C-polysaccharide (PnC) antibody) between the interdigitated electrodes, the gold nanoparticles conjugated with the PnC antibodies were let to bind onto an outer membrane of the bacteria. Upon successfully dense decoration, the bacteria surface will become conductive owing to the metal nanoparticles, and a distinctive conductance change between the electrodes can be observed. Since this success ratio, or the probability of the conductance change, reflects the concentration of the analyte, a number of repeated measurements can be used in the quantification of the bacteria. In this way, we have successfully detected S. pneumoniae in the range of 10–108 CFU/mL. The limit of detection in this work is lower than that in the commercial detection kit. We hope that the nanoparticle decoration method will play a role in the facile detection of various bacteria. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1118 KB)
Share & Cite This Article
Pyo, H.; Lee, C.Y.; Kim, D.; Kim, G.; Lee, S.; Yun, W.S. Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method. Sensors 2017, 17, 2012.
Pyo H, Lee CY, Kim D, Kim G, Lee S, Yun WS. Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method. Sensors. 2017; 17(9):2012.Chicago/Turabian Style
Pyo, Hannah; Lee, Cho Y.; Kim, Daehee; Kim, Gyuhee; Lee, Sangho; Yun, Wan S. 2017. "Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method." Sensors 17, no. 9: 2012.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.