Sensors 2017, 17(12), 2789; https://doi.org/10.3390/s17122789
Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis
Fatimah Syahidah Mohamad, Mohd Hazani Mat Zaid, Jaafar Abdullah, Ruzniza Mohd Zawawi, Hong Ngee Lim
, Yusran Sulaiman and Norizah Abdul Rahman *
, Yusran Sulaiman and Norizah Abdul Rahman *
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
*
Author to whom correspondence should be addressed.
Received: 28 September 2017 / Revised: 2 November 2017 / Accepted: 7 November 2017 / Published: 2 December 2017
(This article belongs to the Section Biosensors)
Abstract
This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10−6–10−9 M with the detection limit of 7.853 × 10−7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors. View Full-TextKeywords:
Mycobacterium tuberculosis; polyaniline; graphene; nanofibers; biosensors
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MDPI and ACS Style
Mohamad, F.S.; Mat Zaid, M.H.; Abdullah, J.; Zawawi, R.M.; Lim, H.N.; Sulaiman, Y.; Abdul Rahman, N. Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis. Sensors 2017, 17, 2789.
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