Next Article in Journal
A Collaboration-Oriented M2M Messaging Mechanism for the Collaborative Automation between Machines in Future Industrial Networks
Next Article in Special Issue
Highly Sensitive FPW-Based Microsystem for Rapid Detection of Tetrahydrocannabinol in Human Urine
Previous Article in Journal
Comparison of Benchtop Fourier-Transform (FT) and Portable Grating Scanning Spectrometers for Determination of Total Soluble Solid Contents in Single Grape Berry (Vitis vinifera L.) and Calibration Transfer
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(11), 2691; https://doi.org/10.3390/s17112691

Dielectrophoretic Separation of Live and Dead Monocytes Using 3D Carbon-Electrodes

1
Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, Turkey
2
Mechanical Engineering Department, Clemson University, Clemson, SC 29631, USA
*
Author to whom correspondence should be addressed.
Received: 19 October 2017 / Revised: 7 November 2017 / Accepted: 13 November 2017 / Published: 22 November 2017
(This article belongs to the Special Issue Bio-MEMS for Precision Medicine)
Full-Text   |   PDF [797 KB, uploaded 22 November 2017]   |  

Abstract

Blood has been the most reliable body fluid commonly used for the diagnosis of diseases. Although there have been promising investigations for the development of novel lab-on-a-chip devices to utilize other body fluids such as urine and sweat samples in diagnosis, their stability remains a problem that limits the reliability and accuracy of readouts. Hence, accurate and quantitative separation and characterization of blood cells are still crucial. The first step in achieving high-resolution characteristics for specific cell subpopulations from the whole blood is the isolation of pure cell populations from a mixture of cell suspensions. Second, live cells need to be purified from dead cells; otherwise, dead cells might introduce biases in the measurements. In addition, the separation and characterization methods being used must preserve the genetic and phenotypic properties of the cells. Among the characterization and separation approaches, dielectrophoresis (DEP) is one of the oldest and most efficient label-free quantification methods, which directly purifies and characterizes cells using their intrinsic, physical properties. In this study, we present the dielectrophoretic separation and characterization of live and dead monocytes using 3D carbon-electrodes. Our approach successfully removed the dead monocytes while preserving the viability of the live monocytes. Therefore, when blood analyses and disease diagnosis are performed with enriched, live monocyte populations, this approach will reduce the dead-cell contamination risk and achieve more reliable and accurate test results. View Full-Text
Keywords: dielectrophoresis; cell separation; carbon-electrode; microfluidics; BioMEMS (biomedical microelectromechanical systems) dielectrophoresis; cell separation; carbon-electrode; microfluidics; BioMEMS (biomedical microelectromechanical systems)
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Yildizhan, Y.; Erdem, N.; Islam, M.; Martinez-Duarte, R.; Elitas, M. Dielectrophoretic Separation of Live and Dead Monocytes Using 3D Carbon-Electrodes. Sensors 2017, 17, 2691.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top