Characterization of the ER-Targeted Low Affinity Ca2+ Probe D4ER
AbstractCalcium ion (Ca2+) is a ubiquitous intracellular messenger and changes in its concentration impact on nearly every aspect of cell life. Endoplasmic reticulum (ER) represents the major intracellular Ca2+ store and the free Ca2+ concentration ([Ca2+]) within its lumen ([Ca2+]ER) can reach levels higher than 1 mM. Several genetically-encoded ER-targeted Ca2+ sensors have been developed over the last years. However, most of them are non-ratiometric and, thus, their signal is difficult to calibrate in live cells and is affected by shifts in the focal plane and artifactual movements of the sample. On the other hand, existing ratiometric Ca2+ probes are plagued by different drawbacks, such as a double dissociation constant (Kd) for Ca2+, low dynamic range, and an affinity for the cation that is too high for the levels of [Ca2+] in the ER lumen. Here, we report the characterization of a recently generated ER-targeted, Förster resonance energy transfer (FRET)-based, Cameleon probe, named D4ER, characterized by suitable Ca2+ affinity and dynamic range for monitoring [Ca2+] variations within the ER. As an example, resting [Ca2+]ER have been evaluated in a known paradigm of altered ER Ca2+ homeostasis, i.e., in cells expressing a mutated form of the familial Alzheimer’s Disease-linked protein Presenilin 2 (PS2). The lower Ca2+ affinity of the D4ER probe, compared to that of the previously generated D1ER, allowed the detection of a conspicuous, more clear-cut, reduction in ER Ca2+ content in cells expressing mutated PS2, compared to controls. View Full-Text
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Greotti, E.; Wong, A.; Pozzan, T.; Pendin, D.; Pizzo, P. Characterization of the ER-Targeted Low Affinity Ca2+ Probe D4ER. Sensors 2016, 16, 1419.
Greotti E, Wong A, Pozzan T, Pendin D, Pizzo P. Characterization of the ER-Targeted Low Affinity Ca2+ Probe D4ER. Sensors. 2016; 16(9):1419.Chicago/Turabian Style
Greotti, Elisa; Wong, Andrea; Pozzan, Tullio; Pendin, Diana; Pizzo, Paola. 2016. "Characterization of the ER-Targeted Low Affinity Ca2+ Probe D4ER." Sensors 16, no. 9: 1419.
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