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Sensors 2016, 16(4), 584;

Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose

School of Public Health, Jilin University, Changchun 130021, China
Author to whom correspondence should be addressed.
Academic Editors: Jong Seung Kim and Min Hee Lee
Received: 1 March 2016 / Revised: 11 April 2016 / Accepted: 19 April 2016 / Published: 22 April 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
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Nanozyme-based chemical sensing is a rapidly emerging field of research. Herein, a simple colorimetric assay for the detection of hydrogen peroxide and glucose based on the peroxidase-like activity of V2O5 nanozymes has been established. In this assay, the effects of pH, substrate, nanozyme concentrations and buffer solution have been investigated. It was found that compared with 3,3′,5,5′-tetramethylbenzidine (TMB), the enzyme substrate o-phenylenediamine (OPD) seriously interfered with the H2O2 detection. Under the optimal reaction conditions, the resulting sensor displayed a good response to H2O2 with a linear range of 1 to 500 μM, and a detection limit of 1 μM at a signal-to-noise ratio of 3. A linear correlation was established between absorbance intensity and concentration of glucose from 10 to 2000 μM, with a detection limit of 10 μM. The current work presents a simple, cheap, more convenient, sensitive, and easy handling colorimetric assay. View Full-Text
Keywords: V2O5 nanozymes; variation; substrates; H2O2; glucose V2O5 nanozymes; variation; substrates; H2O2; glucose

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Sun, J.; Li, C.; Qi, Y.; Guo, S.; Liang, X. Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose. Sensors 2016, 16, 584.

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