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Sensors 2016, 16(4), 574;

Protein Chips for Detection of Salmonella spp. from Enrichment Culture

CNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, Italy
Biotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Benoit Piro
Received: 18 January 2016 / Revised: 18 April 2016 / Accepted: 19 April 2016 / Published: 22 April 2016
(This article belongs to the Section Biosensors)
Full-Text   |   PDF [2381 KB, uploaded 22 April 2016]   |  


Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions. View Full-Text
Keywords: antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp. antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp.

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Poltronieri, P.; Cimaglia, F.; De Lorenzis, E.; Chiesa, M.; Mezzolla, V.; Reca, I.B. Protein Chips for Detection of Salmonella spp. from Enrichment Culture. Sensors 2016, 16, 574.

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