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Sensors 2016, 16(3), 287;

Laccase-Functionalized Graphene Oxide Assemblies as Efficient Nanobiocatalysts for Oxidation Reactions

Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina 45110, Greece
Department of Materials Science and Engineering, University of Ioannina, Ioannina 45110, Greece
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Author to whom correspondence should be addressed.
Academic Editor: Gregory Schneider
Received: 31 December 2015 / Revised: 8 February 2016 / Accepted: 16 February 2016 / Published: 25 February 2016
(This article belongs to the Special Issue Graphene and 2D Material Bionanosensors: Chemistry Matters)
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Multi-layer graphene oxide-enzyme nanoassemblies were prepared through the multi-point covalent immobilization of laccase from Trametes versicolor (TvL) on functionalized graphene oxide (fGO). The catalytic properties of the fGO-TvL nanoassemblies were found to depend on the number of the graphene oxide-enzyme layers present in the nanostructure. The fGO-TvL nanoassemblies exhibit an enhanced thermal stability at 60 °C, as demonstrated by a 4.7-fold higher activity as compared to the free enzyme. The multi-layer graphene oxide-enzyme nanoassemblies can efficiently catalyze the oxidation of anthracene, as well as the decolorization of an industrial dye, pinacyanol chloride. These materials retained almost completely their decolorization activity after five reaction cycles, proving their potential as efficient nano- biocatalysts for various applications. View Full-Text
Keywords: graphene oxide; laccase; nanoassemblies; immobilization; nanobiocatalysis graphene oxide; laccase; nanoassemblies; immobilization; nanobiocatalysis

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Patila, M.; Kouloumpis, A.; Gournis, D.; Rudolf, P.; Stamatis, H. Laccase-Functionalized Graphene Oxide Assemblies as Efficient Nanobiocatalysts for Oxidation Reactions. Sensors 2016, 16, 287.

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