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Open AccessArticle

A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex/Thioflavin T Conformation

Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Sensors 2016, 16(12), 2155;
Received: 3 October 2016 / Revised: 22 November 2016 / Accepted: 24 November 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
PDF [2787 KB, uploaded 16 December 2016]


Pb(II) can cause serious damaging effects to human health, and thus, the study of Pb2+ detection methods to sensitively and selectively monitor Pb(II) pollution has significant importance. In this work, we have developed a label-free fluorescence sensing strategy based on a Pb(II) DNAzyme cleavage and the ThT/G-quadruplex complex. In the presence of Pb(II), a G-rich tail was cut and released from the substrate strand, which then would form a G-quadruplex structure by combination with ThT dye. The fluorescence signal increase was then measured for sensitive Pb(II) quantification with a limit of detection of 0.06 nM. Our sensor also demonstrated high selectivity against six different metal ions, which is very important for the analysis of complex samples. View Full-Text
Keywords: DNAzyme; heavy metal ions; lead ion; thioflavin T (ThT) DNAzyme; heavy metal ions; lead ion; thioflavin T (ThT)

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Wen, Y.; Wang, L.; Li, L.; Xu, L.; Liu, G. A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex/Thioflavin T Conformation. Sensors 2016, 16, 2155.

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