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Open AccessArticle

Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy

Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada
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Author to whom correspondence should be addressed.
Present address: FP Innovations, 2665 East Mall, Vancouver, BC V6T1Z4, Canada
Sensors 2015, 15(1), 1047-1059; https://doi.org/10.3390/s150101047
Received: 4 September 2014 / Accepted: 30 December 2014 / Published: 8 January 2015
(This article belongs to the Special Issue Advances in Optical Biosensors)
A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the “fingerprinting” capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses. View Full-Text
Keywords: micro-Raman spectroscopy; AC electrokinetics; dielectrophoresis; microelectrodes; optical biosensors; M13 phage micro-Raman spectroscopy; AC electrokinetics; dielectrophoresis; microelectrodes; optical biosensors; M13 phage
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MDPI and ACS Style

Tomkins, M.R.; Liao, D.S.; Docoslis, A. Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy. Sensors 2015, 15, 1047-1059.

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