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A Guided Mode Resonance Aptasensor for Thrombin Detection
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Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase

Dipartimento di Scienze Farmaceutiche, University of Padova, via F. Marzolo 5, I-35131 Padova, Italy
Associazione CIVEN, via delle industrie 9, I-30175 Venezia-Marghera, Italy
Author to whom correspondence should be addressed.
Sensors 2011, 11(10), 9426-9441;
Received: 29 July 2011 / Revised: 27 September 2011 / Accepted: 28 September 2011 / Published: 3 October 2011
(This article belongs to the Special Issue Aptamer-Based Sensors)
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We have developed an aptamer-based microarray for human thrombin detection exploiting two non-overlapping DNA thrombin aptamers recognizing different exosites of the target protein. The 15-mer aptamer (TBA1) binds the fibrinogen-binding site, whereas the 29-mer aptamer (TBA2) binds the heparin binding domain. Extensive analysis on the complex formation between human thrombin and modified aptamers was performed by Electrophoresis Mobility Shift Assay (EMSA), in order to verify in solution whether the chemical modifications introduced would affect aptamers/protein recognition. The validated system was then applied to the aptamer microarray, using the solid phase system devised by the solution studies. Finally, the best procedure for Sandwich Aptamer Microarray (SAM) and the specificity of the sandwich formation for the developed aptasensor for human thrombin were optimized. View Full-Text
Keywords: aptamer; thrombin; sandwich aptamer microarray aptamer; thrombin; sandwich aptamer microarray

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sosic, A.; Meneghello, A.; Cretaio, E.; Gatto, B. Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase. Sensors 2011, 11, 9426-9441.

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