Next Article in Journal
Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities
Previous Article in Journal
Taxonomic and Metabolite Diversity of Actinomycetes Associated with Three Australian Ascidians
Previous Article in Special Issue
An eDNA-Based SNP Assay for Ungulate Species and Sex Identification
Open AccessFeature PaperArticle

Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife

Columbia Environmental Research Center, U.S. Geological Survey, Columbia, MO 65201, USA
*
Author to whom correspondence should be addressed.
Diversity 2017, 9(4), 54; https://doi.org/10.3390/d9040054
Received: 2 October 2017 / Revised: 6 November 2017 / Accepted: 15 November 2017 / Published: 21 November 2017
(This article belongs to the Special Issue Application of Environmental DNA for Biological Conservation)
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as a survey tool to examine the potential use of uranium mine containment ponds as water sources by wildlife. We tested samples from surface water near mines and from one mine containment pond using two markers, 12S and 16S rRNA gene amplicons, to survey for vertebrate species. We recovered large numbers of sequence reads from taxa expected to be in the area and from less common or hard to observe taxa such as the tiger salamander and gray fox. Detection of these two species is of note because they were not observed in a previous species assessment, and tiger salamander DNA was found in the mine containment pond sample. We also found that sample concentration by centrifugation was a more efficient and more feasible method than filtration in these highly turbid surface waters. Ultimately, the use of eDNA metabarcoding could allow for a better understanding of the area’s overall biodiversity and community composition as well as aid current ecotoxicological risk assessment work. View Full-Text
Keywords: biodiversity surveys; vertebrates; wildlife attraction nuisance; ecotoxicology biodiversity surveys; vertebrates; wildlife attraction nuisance; ecotoxicology
Show Figures

Figure 1

MDPI and ACS Style

Klymus, K.E.; Richter, C.A.; Thompson, N.; Hinck, J.E. Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife. Diversity 2017, 9, 54.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop