Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Collection and Morphological Analyses
2.2. DNA Extraction, PCR Amplification and DNA Sequencing
2.3. Molecular Analyses
Access | Species | Host | Country | Stage | Reference |
---|---|---|---|---|---|
DQ116432 | Skrjabinisakis physeteris | Physeter macrocephalus | Mediterranean Sea | A | [33] |
AB592801 | Skrjabinisakis physeteris | Beryx splendens | Japan | L | [34] |
OR192868 | Skrjabinisakis physeteris (Sphy1) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192869 | Skrjabinisakis physeteris (Sphy2) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192870 | Skrjabinisakis physeteris (Sphy3) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192871 | Skrjabinisakis physeteris (Sphy4) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192872 | Skrjabinisakis physeteris (Sphy5) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192873 | Skrjabinisakis physeteris (Sphy6) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
MH669506 | Skrjabinisakis brevispiculata | Diaphus sp. | Indian Ocean | L | [35] |
DQ116433 | Skrjabinisakis brevispiculata | Kogia breviceps | No registred | A | [33] |
OR192874 | Skrjabinisakis brevispiculata (Sbre1) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192875 | Skrjabinisakis brevispiculata (Sbre2) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192876 | Skrjabinisakis brevispiculata (Sbre3) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192877 | Skrjabinisakis brevispiculata (Sbre4) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
DQ116434 | Skrjabinisakis paggiae | Kogia breviceps | West Atlantic Ocean (Florida coast) | A | [33] |
AB592807 | Skrjabinisakis paggiae | Beryx splendens | Japan | L | [34] |
DQ116430 | Anisakis ziphidarum | Mesoplodon layardii | Southeast Atlantic Ocean (South African coast) | A | [33] |
AB517573 | Anisakis ziphidarum | Scomber japonicus | Japan | L | [36] |
DQ116431 | Anisakis nascetti | Mesoplodon miros | Southeast Atlantic Ocean (South African coast) | A | [33] |
GQ118167 | Anisakis nascetti | Mesoplodon grayi | From off New Zealand | A | [37] |
DQ116427 | Anisakis typica | Delphinidae | Western North Atlantic Ocean | A | [33] |
KC928266 | Anisakis typica | Katsuwonus pelamis | Southern Makassar Strait, Indonesia | L | [38] |
KC810003 | Anisakis simplex | Balaenoptera acutorostrata | Northeastern Atlantic Ocean (Norwegian coast) | A | [39] |
DQ116426 | Anisakis simplex | Delphinidae | Northeast Pacific coast | A | [33] |
MZ546440 | Anisakis pegreffii | Seriolella violacea | Southeastern Pacific Ocean | L | [20] |
DQ116428 | Anisakis pegreffii | Delphinus delphis | Northeast Atlantic Ocean (Spanish coast) | A | [33] |
OR192866 | Anisakis pegreffii (Apeg1) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
OR192867 | Anisakis pegreffii (Apeg2) | Genypterus maculatus | Southeastern Pacific Ocean | L | Present study |
MN385245 | Anisakis berlandi | Globicephala melas | New Zealand | A | [40] |
KC810000 | Anisakis berlandi | Globicephala melas | New Zealand | A | [39] |
JQ934891 | Hysterothylacium aduncum * | Trachurus trachurus | Croatia | L | [41] |
3. Results
3.1. Systematics and Morphological Characteristics of the Anisakid Larvae
- Class Chromadorea Inglis, 1983.
- Order Rhabditida Chitwood, 1933.
- Anisakidae Railliet and Henry, 1912.
3.1.1. Anisakis pegreffii Campana-Rouget and Biocca, 1955 (Figure 1A,B and Figure 2A,B)
- Site in host: body cavity.
- Specimens deposited: Hologenophore (MUSM-HEL 5141).
- Representative DNA sequence: Sequences were deposited in GenBank under the accession numbers OR192866 and OR192867 for the mtDNA cox2.
3.1.2. Skrjabinisakis brevispiculata (Dollfus, 1966) Safonova, Voronova and Vainutis, 2021 (Figure 1C,D and Figure 2C,D)
- Site in host: body cavity.
- Specimens deposited: Hologenophore (MUSM-HEL 5142).
- Representative DNA sequence: Sequences were deposited in GenBank under the accession numbers OR192874–OR192877 for the mtDNA cox2.
3.1.3. Skrjabinisakis physeteris (Baylis, 1923) Safonova, Voronova and Vainutis, 2021 (Figure 1E,F and Figure 2E,F)
- Site in host: body cavity.
- Specimens deposited: Hologenophore (MUSM-HEL 5143).
- Representative DNA sequence: Sequences were deposited in GenBank under the accession numbers OR192868–OR192873 for the mtDNA cox2.
3.2. Phylogenetic Analyses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chero, J.D.; Ñacari, L.; Cruces, C.L.; Lopez, D.F.; Cacique, E.; Severino, R.; Lopez, J.; Luque, J.L.; Saéz, G. Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru. Diversity 2023, 15, 820. https://doi.org/10.3390/d15070820
Chero JD, Ñacari L, Cruces CL, Lopez DF, Cacique E, Severino R, Lopez J, Luque JL, Saéz G. Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru. Diversity. 2023; 15(7):820. https://doi.org/10.3390/d15070820
Chicago/Turabian StyleChero, Jhon Darly, Luis Ñacari, Celso Luis Cruces, David Fermín Lopez, Edson Cacique, Ruperto Severino, Jorge Lopez, José Luis Luque, and Gloria Saéz. 2023. "Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru" Diversity 15, no. 7: 820. https://doi.org/10.3390/d15070820