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Volume 14
Issue 5
10.3390/d14050369
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Article

Cytochrome Oxidase Subunit II: Potential Marker for the Identification of Forensically Significant Species of Coleoptera—A Preliminary Study

by
Neha Singh
1,
Drishtant Singh
2,
Anup Kumar Kesavan
3,
Nadiyah M. Alabdallah
4,
Mohammed A. Alshehri
5,
Samy Sayed
6,
Mohammad Javed Ansari
7,* and
Madhu Bala
1,*
1
Department of Zoology & Environmental Sciences, Punjabi University, Patiala 147001, India
2
Department of Molecular Biology & Biochemistry, Guru Nanak Dev University, Amritsar 143005, India
3
Department of Biotechnology and Microbiology, Dr. Janaki Ammal Campus, Kannur University, Palavad, Thalassery 670661, India
4
Department of Biology, College of Science, Imam Abulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
5
Biology Department, College of Science, University of Tabuk, Tabuk 71491, Saudi Arabia
6
Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia
7
Department of Botany, Hindu College Moradabad, Mahatma Jyotiba Phule Rohilkhand University, Bareilly 244001, India
*
Authors to whom correspondence should be addressed.
Diversity 2022, 14(5), 369; https://doi.org/10.3390/d14050369
Submission received: 5 March 2022 / Revised: 2 May 2022 / Accepted: 3 May 2022 / Published: 6 May 2022
(This article belongs to the Special Issue Beetles Phylogeny, Evolution, and Diversity: Past, Present, and Future)
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Abstract

The foremost concern in forensic entomology is the explicit identification of the species recovered from the crime scene. From the different orders of insects, Diptera is the prime focus in this field, followed by Coleoptera, whose identification can be extremely helpful for corpses in later decomposition stages. In this study, cytochrome oxidase subunit II (COII) was used to check its adequacy as a genetic marker and to create a reference database for eleven species belonging to five families of Coleoptera, namely, Silphidae, Staphylinidae, Histeridae, Dermestidae and Scarabaeidae, from two different states in India to assist in the accurate identification of imperative beetle species in medico-legal entomology. To achieve this, standard protocols of DNA isolation, amplification and sequencing were followed. We concluded that the COII gene can be used as a molecular marker for the identification of forensically relevant species, as observed from the similarities between the phylogenetic relationship constructed by COII and morphological data.
Keywords: beetles; COII gene; forensic entomology; Coleoptera; species identification beetles; COII gene; forensic entomology; Coleoptera; species identification

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MDPI and ACS Style

Singh, N.; Singh, D.; Kesavan, A.K.; Alabdallah, N.M.; Alshehri, M.A.; Sayed, S.; Ansari, M.J.; Bala, M. Cytochrome Oxidase Subunit II: Potential Marker for the Identification of Forensically Significant Species of Coleoptera—A Preliminary Study. Diversity 2022, 14, 369. https://doi.org/10.3390/d14050369

AMA Style

Singh N, Singh D, Kesavan AK, Alabdallah NM, Alshehri MA, Sayed S, Ansari MJ, Bala M. Cytochrome Oxidase Subunit II: Potential Marker for the Identification of Forensically Significant Species of Coleoptera—A Preliminary Study. Diversity. 2022; 14(5):369. https://doi.org/10.3390/d14050369

Chicago/Turabian Style

Singh, Neha, Drishtant Singh, Anup Kumar Kesavan, Nadiyah M. Alabdallah, Mohammed A. Alshehri, Samy Sayed, Mohammad Javed Ansari, and Madhu Bala. 2022. "Cytochrome Oxidase Subunit II: Potential Marker for the Identification of Forensically Significant Species of Coleoptera—A Preliminary Study" Diversity 14, no. 5: 369. https://doi.org/10.3390/d14050369

APA Style

Singh, N., Singh, D., Kesavan, A. K., Alabdallah, N. M., Alshehri, M. A., Sayed, S., Ansari, M. J., & Bala, M. (2022). Cytochrome Oxidase Subunit II: Potential Marker for the Identification of Forensically Significant Species of Coleoptera—A Preliminary Study. Diversity, 14(5), 369. https://doi.org/10.3390/d14050369

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