Next Article in Journal
Building a Robust, Densely-Sampled Spider Tree of Life for Ecosystem Research
Next Article in Special Issue
Biodiversity of Ciliates and Their Symbionts: A Special Issue
Previous Article in Journal
Rotating Arrays of Orchid Flowers: A Simple and Effective Method for Studying Pollination in Food Deceptive Plants
Previous Article in Special Issue
Effectiveness of Photoprotective Strategies in Three Mixotrophic Planktonic Ciliate Species
Open AccessArticle

Algal Diversity in Paramecium bursaria: Species Identification, Detection of Choricystis parasitica, and Assessment of the Interaction Specificity

1
Microbiology, Institute of Biology II, Albert Ludwig University of Freiburg, 79104 Freiburg, Germany
2
Department of Microbiology, Faculty of Biology, Saint Petersburg State University, 199034 Saint Petersburg, Russia
3
Laboratory of Cellular and Molecular Protistology, Zoological Institute RAS, 199034 Saint Petersburg, Russia
4
Research Department for Limnology, University of Innsbruck, 5310 Mondsee, Austria
*
Author to whom correspondence should be addressed.
Diversity 2020, 12(8), 287; https://doi.org/10.3390/d12080287
Received: 6 May 2020 / Revised: 17 July 2020 / Accepted: 19 July 2020 / Published: 23 July 2020
(This article belongs to the Special Issue Biodiversity of Ciliates and their Symbionts)
The ‘green’ ciliate Paramecium bursaria lives in mutualistic symbiosis with green algae belonging to the species Chlorella variabilis or Micractinium conductrix. We analysed the diversity of algal endosymbionts and their P. bursaria hosts in nine strains from geographically diverse origins. Therefore, their phylogenies using different molecular markers were inferred. The green paramecia belong to different syngens of P. bursaria. The intracellular algae were assigned to Chl. variabilis, M. conductrix or, surprisingly, Choricystis parasitica. This usually free-living alga co-occurs with M. conductrix in the host’s cytoplasm. Addressing the potential status of Chor. parasitica as second additional endosymbiont, we determined if it is capable of symbiosis establishment and replication within a host cell. Symbiont-free P. bursaria were generated by cycloheximid treatment. Those aposymbiotic P. bursaria were used for experimental infections to investigate the symbiosis specificity not only between P. bursaria and Chor. parasitica but including also Chl. variabilis and M. conductrix. For each algae we observed the uptake and incorporation in individual perialgal vacuoles. These host-symbiont associations are stable since more than five months. Thus, Chor. parasitica and P. bursaria can form an intimate and long-term interaction. This study provides new insights into the diversity of P. bursaria algal symbionts. View Full-Text
Keywords: Chlorella; endosymbiosis; intracellular algae; Micractinium; photobiont; infection; syngen Chlorella; endosymbiosis; intracellular algae; Micractinium; photobiont; infection; syngen
Show Figures

Graphical abstract

MDPI and ACS Style

Flemming, F.E.; Potekhin, A.; Pröschold, T.; Schrallhammer, M. Algal Diversity in Paramecium bursaria: Species Identification, Detection of Choricystis parasitica, and Assessment of the Interaction Specificity. Diversity 2020, 12, 287. https://doi.org/10.3390/d12080287

AMA Style

Flemming FE, Potekhin A, Pröschold T, Schrallhammer M. Algal Diversity in Paramecium bursaria: Species Identification, Detection of Choricystis parasitica, and Assessment of the Interaction Specificity. Diversity. 2020; 12(8):287. https://doi.org/10.3390/d12080287

Chicago/Turabian Style

Flemming, Felicitas E.; Potekhin, Alexey; Pröschold, Thomas; Schrallhammer, Martina. 2020. "Algal Diversity in Paramecium bursaria: Species Identification, Detection of Choricystis parasitica, and Assessment of the Interaction Specificity" Diversity 12, no. 8: 287. https://doi.org/10.3390/d12080287

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop