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Volume 27
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles

by
Anna Epanchintseva
1,*,
Julia Shyiskaya
1,
Julia Poletaeva
1,
Ekaterina Baeva
1,
Evgenia Kozyreva
1,
Evgeniya Dyudeyeva
1,
Mariya Khokhlova
2 and
Inna Pyshnaya
1,*
1
Knorre Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
2
Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2026, 27(19), 8809; https://doi.org/10.3390/ijms27198809 (registering DOI)
Submission received: 31 August 2026 / Revised: 25 September 2026 / Accepted: 29 September 2026 / Published: 1 October 2026
(This article belongs to the Special Issue Physicochemical Properties and Applications of Nanomaterials in Biology and Medicine: 2nd Edition)
Versions Notes

Abstract

Detection of single-nucleotide mutations (SNPs) in the genome of infectious agents is one of the ways to identify their drug-resistant strains. Antibiotic-resistant strains of Mycobacterium tuberculosis (MTB) impede advances in the fight against the spread of tuberculosis. Diagnostic tools exist for both MTB itself and its drug-resistant strains, most of which are technically complex, resource-intensive, and time-consuming. This paper presents the development of a colorimetric test system, requiring no expensive equipment, for the rapid and accurate determination of the SNPs associated with drug-resistant forms of MTB to fluoroquinolones in modeled DNA targets (198 bp). For this purpose, nanoconstructs based on gold nanoparticles (AuNPs) and hybrid magnetic Fe3O4@Au NPs were created, leveraging the advantages of each nanomaterial. The use of AuNPs provides a basis for colorimetric detection due to their high optical absorption. Fe3O4@Au NPs enable samples to respond to an external magnetic field. The study demonstrates the ability to reliably discriminate between wild-type and mutant-model DNA targets in the nanomolar concentration range within 15 min. Visual analysis and optical absorption spectroscopy were used to evaluate the detection results. The application of an external magnetic field allows for enhanced colorimetric distinction between wild-type target samples and those containing an SNP.
Keywords: magnetic hybrid nanoparticles; Fe3O4@Au; core–satellite; magnetite; gold nanoparticles; drug-resistant forms of tuberculosis; single-nucleotide polymorphism; fluoroquinolones; tandem duplexes; noncovalent adsorption; colorimetric detection magnetic hybrid nanoparticles; Fe3O4@Au; core–satellite; magnetite; gold nanoparticles; drug-resistant forms of tuberculosis; single-nucleotide polymorphism; fluoroquinolones; tandem duplexes; noncovalent adsorption; colorimetric detection

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MDPI and ACS Style

Epanchintseva, A.; Shyiskaya, J.; Poletaeva, J.; Baeva, E.; Kozyreva, E.; Dyudeyeva, E.; Khokhlova, M.; Pyshnaya, I. A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles. Int. J. Mol. Sci. 2026, 27, 8809. https://doi.org/10.3390/ijms27198809

AMA Style

Epanchintseva A, Shyiskaya J, Poletaeva J, Baeva E, Kozyreva E, Dyudeyeva E, Khokhlova M, Pyshnaya I. A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles. International Journal of Molecular Sciences. 2026; 27(19):8809. https://doi.org/10.3390/ijms27198809

Chicago/Turabian Style

Epanchintseva, Anna, Julia Shyiskaya, Julia Poletaeva, Ekaterina Baeva, Evgenia Kozyreva, Evgeniya Dyudeyeva, Mariya Khokhlova, and Inna Pyshnaya. 2026. "A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles" International Journal of Molecular Sciences 27, no. 19: 8809. https://doi.org/10.3390/ijms27198809

APA Style

Epanchintseva, A., Shyiskaya, J., Poletaeva, J., Baeva, E., Kozyreva, E., Dyudeyeva, E., Khokhlova, M., & Pyshnaya, I. (2026). A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles. International Journal of Molecular Sciences, 27(19), 8809. https://doi.org/10.3390/ijms27198809

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