Next Article in Journal
Disturbances of Mitochondrial Functions and Oxidative Stress Induction in Methylmalonic and Propionic Acidemias: A Critical Role for Metabolite Accumulation
Previous Article in Journal
Colitis-Associated Colorectal Cancer—STAT3 Inhibition as a Preventive Strategy
Previous Article in Special Issue
Molecular Mechanisms of NF-Y Transcription Factors in Horticultural Plant Development and Stress Responses: Recent Advances
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 27
Issue 18
10.3390/ijms27188380
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Transcriptomic and Targeted Carotenoid Metabolomic Dissection of Pod Color Difference Between Wild Type and an EMS-Induced Stable pcm Mutant in Snap Bean (Phaseolus vulgaris L.)

by
Qiuhui Mu
†,
Hongning Wu
†,
Guojun Feng
,
Xiaoxu Yang
,
Dajun Liu
,
Zhishan Yan
,
Taifeng Zhang
and
Chang Liu
*
College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2026, 27(18), 8380; https://doi.org/10.3390/ijms27188380 (registering DOI)
Submission received: 11 August 2026 / Revised: 14 September 2026 / Accepted: 17 September 2026 / Published: 20 September 2026
(This article belongs to the Special Issue Transcriptional Regulation in Plant Development: 3rd Edition)
Browse Figures
Versions Notes

Abstract

Pod color is an essential economic trait of snap bean (Phaseolus vulgaris L.), and golden-yellow pods are more commercially popular than light-yellow varieties. Exploring the molecular regulatory mechanism underlying deep-yellow pod formation is essential for snap bean quality improvement. In this study, the wild-type cultivar Jinguan and the EMS-derived deep-yellow pod mutant pcm were used as materials. Integrative analyses of photosynthetic pigment quantification, genetic analysis, transcriptome sequencing, and targeted carotenoid metabolomics were performed to identify key genes and metabolic pathways governing pod color variation. Genetic analysis revealed that the deep-yellow pod trait of the pcm is stably inherited and controlled by a nuclear recessive gene. Transcriptome screening identified three differentially expressed key genes, PSY (Phvul.006G024100g), CCD4 (Phvul.002G120600g), and CYP707A (Phvul.002G122200g), whose expression patterns were strongly correlated with carotenoid accumulation. Metabolomic results demonstrated that the pcm presented reduced upstream β-carotene content, while the synthesis and accumulation of downstream chromogenic carotenoids, including zeaxanthin and capsorubin, were significantly enhanced, resulting in elevated total carotenoid content. Collectively, the differential expression of these genes remodels carotenoid metabolism and ultimately contributes to the deep-yellow pod phenotype. This study explores the regulatory mechanism of carotenoid metabolism in pod color formation, providing valuable theoretical support and genetic resources for quality breeding of snap beans.
Keywords: snap bean; deep-yellow pod color mutant; carotenoid; transcriptome sequencing; metabolome sequencing snap bean; deep-yellow pod color mutant; carotenoid; transcriptome sequencing; metabolome sequencing

Share and Cite

MDPI and ACS Style

Mu, Q.; Wu, H.; Feng, G.; Yang, X.; Liu, D.; Yan, Z.; Zhang, T.; Liu, C. Transcriptomic and Targeted Carotenoid Metabolomic Dissection of Pod Color Difference Between Wild Type and an EMS-Induced Stable pcm Mutant in Snap Bean (Phaseolus vulgaris L.). Int. J. Mol. Sci. 2026, 27, 8380. https://doi.org/10.3390/ijms27188380

AMA Style

Mu Q, Wu H, Feng G, Yang X, Liu D, Yan Z, Zhang T, Liu C. Transcriptomic and Targeted Carotenoid Metabolomic Dissection of Pod Color Difference Between Wild Type and an EMS-Induced Stable pcm Mutant in Snap Bean (Phaseolus vulgaris L.). International Journal of Molecular Sciences. 2026; 27(18):8380. https://doi.org/10.3390/ijms27188380

Chicago/Turabian Style

Mu, Qiuhui, Hongning Wu, Guojun Feng, Xiaoxu Yang, Dajun Liu, Zhishan Yan, Taifeng Zhang, and Chang Liu. 2026. "Transcriptomic and Targeted Carotenoid Metabolomic Dissection of Pod Color Difference Between Wild Type and an EMS-Induced Stable pcm Mutant in Snap Bean (Phaseolus vulgaris L.)" International Journal of Molecular Sciences 27, no. 18: 8380. https://doi.org/10.3390/ijms27188380

APA Style

Mu, Q., Wu, H., Feng, G., Yang, X., Liu, D., Yan, Z., Zhang, T., & Liu, C. (2026). Transcriptomic and Targeted Carotenoid Metabolomic Dissection of Pod Color Difference Between Wild Type and an EMS-Induced Stable pcm Mutant in Snap Bean (Phaseolus vulgaris L.). International Journal of Molecular Sciences, 27(18), 8380. https://doi.org/10.3390/ijms27188380

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.
Back to TopTop