Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering
Abstract
:1. Introduction
2. Results
2.1. Analysis of Sorption Capacity
2.2. Microscopic Observations with Determination of Roughness Profile
2.3. Results of the Incubation Study
2.4. Results of Infrared Spectroscopy Analysis
2.5. Characterization of Hydrogels via Microscopic Techniques
2.6. Analysis of the Density of Polymeric Materials
2.7. Synthesis and Characterization of Polymeric Material Modified with Mixture of Bioactive Compounds
3. Materials and Methods
3.1. Materials
3.2. Synthesis of Hydrogel Materials
3.3. Sorption Capacity Analysis
3.4. Incubation Studies
3.5. Microscopic Observations and Surface Roughness Profile Analysis
3.6. Infrared Spectroscopy Analysis
3.7. Analysis of the Density of Polymeric Materials
3.8. Characterization of Hydrogels via Microscopic Techniques
3.9. Synthesis and Characterization of Polymeric Material Modified with Mixture of Bioactive Compounds
4. Conclusions
- The swelling capacity of hydrogels is significantly influenced by the amount and molecular weight of PEGDA. Lower amounts of PEGDA and lower molecular weight (575 g/mol) resulted in higher swelling coefficients due to a looser network structure, while higher amounts of PEGDA with higher molecular weight (700 g/mol) produced denser networks with reduced water absorption.
- Increased amounts of PEGDA lead to higher surface roughness both before and after incubation, with significant changes observed in samples containing PEGDA 575 g/mol, highlighting their susceptibility to structural alterations. PEGDA 700 g/mol provided better stability and resistance to degradation.
- The pH stability of the hydrogels was largely unaffected by incubation in distilled water and citric acid, confirming the absence of degradation. However, a significant pH jump was observed in artificial saliva, likely due to interactions with its complex composition, without evidence of hydrogel degradation. Such interactions underscore the importance of the chemical environment in determining hydrogel performance.
- FT-IR spectroscopy confirmed the presence of characteristic functional groups of both the hydrogel matrix and bioactive mixture. The integration of bioactive components (glucosamine, chondroitin, MSM) was successful, as indicated by new and intensified absorption bands. These modifications suggest potential improved bioactivity and compatibility.
- The study demonstrates that the developed hydrogels, particularly those modified with bioactive compounds, have significant potential for applications in regenerative medicine, such as joint disease treatment and cartilage repair, due to their customizable properties, biocompatibility, and chemical stability. Further studies are recommended to optimize their properties.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Name | Ra [µm] | Rz [µm] |
---|---|---|
2_575 | 2.43 | 17.42 |
2.5_575 | 14.32 | 153.47 |
2_700 | 5.12 | 25.52 |
2.5_700 | 13.42 | 80.20 |
15% PVP 10 k [mL] | 5% PVA 13–23 k [mL] | PEGDA [mL] | Photoinitiator [µL] | Sample Name * |
---|---|---|---|---|
3 | 7 | 2 (700) | 50 | 2_700 |
2.5 (700) | 2.5_700 | |||
2 (575) | 2_575 | |||
2.5 (575) | 2.5_575 |
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Wanat, D.; Garbowska, C.; Wrzesińska, W.; Grzywacz, O.; Sala, K.; Zapotoczny, K.; Bańkosz, M.; Jampilek, J.; Walter, J.; Tyliszczak, B. Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering. Int. J. Mol. Sci. 2025, 26, 2057. https://doi.org/10.3390/ijms26052057
Wanat D, Garbowska C, Wrzesińska W, Grzywacz O, Sala K, Zapotoczny K, Bańkosz M, Jampilek J, Walter J, Tyliszczak B. Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering. International Journal of Molecular Sciences. 2025; 26(5):2057. https://doi.org/10.3390/ijms26052057
Chicago/Turabian StyleWanat, Dominika, Claudia Garbowska, Wiktoria Wrzesińska, Oliwia Grzywacz, Katarzyna Sala, Kacper Zapotoczny, Magdalena Bańkosz, Josef Jampilek, Janusz Walter, and Bożena Tyliszczak. 2025. "Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering" International Journal of Molecular Sciences 26, no. 5: 2057. https://doi.org/10.3390/ijms26052057
APA StyleWanat, D., Garbowska, C., Wrzesińska, W., Grzywacz, O., Sala, K., Zapotoczny, K., Bańkosz, M., Jampilek, J., Walter, J., & Tyliszczak, B. (2025). Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering. International Journal of Molecular Sciences, 26(5), 2057. https://doi.org/10.3390/ijms26052057