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Article

Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses

by
Pritsana Raungrut
1,*,
Jirapon Jirapongsak
1,
Suchanan Tanyapattrapong
1,
Thitaya Bunsong
2,
Thidarat Ruklert
2,
Kannika Kueakool
3,
Paramee Thongsuksai
4 and
Narongwit Nakwan
2,*
1
Division of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand
2
Division of Pulmonology, Department of Medicine, Hat Yai Medical Education Center, Hat Yai Hospital, Hat Yai 90112, Songkhla, Thailand
3
Faculty of Medicine, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand
4
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(3), 1010; https://doi.org/10.3390/ijms26031010
Submission received: 9 December 2024 / Revised: 15 January 2025 / Accepted: 22 January 2025 / Published: 24 January 2025
(This article belongs to the Special Issue Molecular Research of Multi-omics in Cancer)
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Abstract

Cisplatin combined with gemcitabine, a doublet regimen, is the first-line treatment for patients with advanced lung adenocarcinoma (ADC); however, the treatment response remains poor. This study aimed to identify potential biomarkers for predicting response to cisplatin and gemcitabine. Tissue transcriptome and blood proteome analyses were conducted on 27 patients with lung ADC. Blood-derived proteins that reflected tissue-specific biomarkers were obtained using Venn diagrams. The candidate proteins were validated by Western blotting. Lentivirus-mediated short hairpin RNA interference was used to verify the functional roles of the candidate proteins in human A549 cells. We identified 417 differentially expressed genes, including 52 upregulated and 365 downregulated genes, and 31 differentially expressed proteins, including 26 upregulated and 5 downregulated proteins. Integrative analysis revealed the presence of alpha-1-acid glycoprotein 1 (A1AG1) and fibrinogen alpha chain (FGA or FIBA) in both the tissue and serum. FGA levels were elevated in responders compared to non-responders, and reduced serum FGA levels were correlated with resistance to this regimen. Moreover, FGA knockdown in A549 cells resulted in resistance to the doublet regimen. Our findings indicate that FGA is a tissue-specific serum protein that may function as a blood-based biomarker to predict the response of patients with lung ADC to cisplatin plus gemcitabine chemotherapy.
Keywords: fibrinogen alpha chain; lung adenocarcinoma; transcriptome; proteome; chemotherapy fibrinogen alpha chain; lung adenocarcinoma; transcriptome; proteome; chemotherapy

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MDPI and ACS Style

Raungrut, P.; Jirapongsak, J.; Tanyapattrapong, S.; Bunsong, T.; Ruklert, T.; Kueakool, K.; Thongsuksai, P.; Nakwan, N. Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses. Int. J. Mol. Sci. 2025, 26, 1010. https://doi.org/10.3390/ijms26031010

AMA Style

Raungrut P, Jirapongsak J, Tanyapattrapong S, Bunsong T, Ruklert T, Kueakool K, Thongsuksai P, Nakwan N. Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses. International Journal of Molecular Sciences. 2025; 26(3):1010. https://doi.org/10.3390/ijms26031010

Chicago/Turabian Style

Raungrut, Pritsana, Jirapon Jirapongsak, Suchanan Tanyapattrapong, Thitaya Bunsong, Thidarat Ruklert, Kannika Kueakool, Paramee Thongsuksai, and Narongwit Nakwan. 2025. "Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses" International Journal of Molecular Sciences 26, no. 3: 1010. https://doi.org/10.3390/ijms26031010

APA Style

Raungrut, P., Jirapongsak, J., Tanyapattrapong, S., Bunsong, T., Ruklert, T., Kueakool, K., Thongsuksai, P., & Nakwan, N. (2025). Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses. International Journal of Molecular Sciences, 26(3), 1010. https://doi.org/10.3390/ijms26031010

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