Next Article in Journal
DNA-Programmable Oligonucleotide Insecticide Eriola-11 Targets Mitochondrial 16S rRNA and Exhibits Strong Insecticidal Activity Against Woolly Apple Aphid (Eriosoma lanigerum) Hausmann
Previous Article in Journal
Genome-Editing Tools for Lactic Acid Bacteria: Past Achievements, Current Platforms, and Future Directions
Previous Article in Special Issue
Enhancing Tissue Integration and Reducing Inflammation in Silicone and Human Acellular Dermal Matrix Implants via Vacuum Plasma Treatment
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 26
Issue 15
10.3390/ijms26157480
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations

by
Hanne Devos
1,2,†,
Ioanna K. Mina
3,4,
Foteini Paradeisi
1,
Manousos Makridakis
1,
Aggeliki Tserga
1,
Marika Mokou
3,
Jerome Zoidakis
1,
Harald Mischak
3,
Antonia Vlahou
1,
Agnieszka Latosinska
3,* and
Maria G. Roubelakis
2,5,*
1
Centre of Systems Biology, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece
2
Laboratory of Biology, University of Athens School of Medicine, 11527 Athens, Greece
3
Mosaiques Diagnostics GmbH, 30659 Hannover, Germany
4
Institute for Molecular Cardiovascular Research, University Hospital RWTH Aachen, 52074 Aachen, Germany
5
Laboratory of Cell and Gene Therapy, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece
*
Authors to whom correspondence should be addressed.
†
Current address: Department of Cardiovascular Disease, Nordic Bioscience A/S, DK-2730 Herlev, Denmark.
Int. J. Mol. Sci. 2025, 26(15), 7480; https://doi.org/10.3390/ijms26157480
Submission received: 23 June 2025 / Revised: 22 July 2025 / Accepted: 31 July 2025 / Published: 2 August 2025
(This article belongs to the Special Issue The Role of Extracellular Matrix Proteins in Pathogenesis: 2nd Edition)
Browse Figures
Versions Notes

Abstract

Collagen type I (COL(I)) is a key component of the extracellular matrix (ECM) and is involved in cell signaling and migration through cell receptors. Collagen degradation produces bioactive peptides (matrikines), which influence cellular processes. In this study, we investigated the biological effects of nine most abundant, naturally occurring urinary COL(I)-derived peptides on human endothelial cells at physiological concentrations, using cell migration assays, mass spectrometry-based proteomics, flow cytometry, and AlphaFold 3. While none of the peptides significantly altered endothelial migration by themselves at physiological concentrations, full-length COL(I) increased cell migration, which was inhibited by Peptide 1 (229NGDDGEAGKPGRPGERGPpGp249). This peptide uniquely contains the DGEA and GRPGER motifs, interacting with integrin α2β1. Flow cytometry confirmed the presence of integrin α2β1 on human endothelial cells, and AlphaFold 3 modeling predicted an interaction between Peptide 1 and integrin α2. Mass spectrometry-based proteomics investigating signaling pathways revealed that COL(I) triggered phosphorylation events linked to integrin α2β1 activation and cell migration, which were absent in COL(I) plus peptide 1-treated cells. These findings identify Peptide 1 as a biologically active COL(I)-derived peptide at a physiological concentration capable of modulating collagen-induced cell migration, and provide a foundation for further investigation into its mechanisms of action and role in urine excretion.

1. Introduction

The extracellular matrix (ECM) is a specialized three-dimensional network, that plays a critical role in cell proliferation, survival, migration and differentiation, through interactions with various cell receptors [1]. Among the ECM’s components, collagen type I (COL(I)) is one of the most abundant proteins in both the ECM and the human body [2]. Dysregulation of COL(I) has been implicated in numerous diseases [3], most notably fibrotic conditions characterized by an imbalance between collagen synthesis and degradation [2]. COL(I) is composed of two alpha 1 chains (COLα1 (I)) and one alpha 2 chain (COLα2 (I)). Upon maturation, COL(I) molecules are exported to the ECM, where they form cross-links—primarily between hydroxylated proline and lysine residues—to create robust collagen fibrils. These fibrils interact with cell surface receptors, such as integrin α1β1 and integrin α2β1 [2,4]. Activation of these receptors by collagen initiates intracellular signaling cascades, including mobilization of Src kinase and activation of Focal Adhesion Kinase (FAK) [5,6]. Downstream pathways involving key signaling proteins like Extracellular-signal Regulated Kinase (ERK) and Akt are subsequently activated [6,7,8,9,10,11,12,13], promoting processes such as cell migration, proliferation and angiogenesis [5,7,8,10,14,15].
Matrix-metalloproteinases (MMPs) [16] and cathepsins [17] are prominently involved in the degradation of ECM proteins. This degradation generates peptides, some of which exhibit biological activity and are termed “matrikines”. These matrikines can circulate in blood and are frequently detectable in urine [18,19]. Their identification is facilitated by technologies such as capillary electrophoresis coupled with mass spectrometry (CE-MS), a robust and reproducible high-throughput method [20]. Biological activities of collagen-derived matrikines were reported in the context of ECM deposition, collagen synthesis, chemo-attraction, proliferation, and inflammation [18]. Specific motifs on these peptides, such as the DGEA motif, have been extensively studied. Synthetic peptides containing the DGEA motif interact with integrin α2β1 [21], activating pathways involving Signal Transducer and Activator of Transcription 6 (STAT6) [22], c Jun N-terminal kinase (Jnk) [23], Akt [24], and calcium mobilization through inositol 1,4,5-triphosphate (IP3) and phospholipase C (PLC) [25,26,27]. Functionally, DGEA has been shown to inhibit endothelial cell migration [28], suppress angiogenesis [29], influence macrophage polarization towards an M2 phenotype [22,30], and induce osteogenic differentiation [23,31,32] and cell adhesion [21,26,31]. Other motifs, such as GUXGEZ (where U is usually hydrophobic, X usually hydroxyproline, and Z is often arginine), also interact with integrin receptors [33]. Prominent examples include GFOGER, GROGER and GLOGER motifs (with ‘O’ denoting hydroxyproline), which bind to integrin α1β1 (GFOGER, GROGER) and α2β1 (GFOGER, GLOGER, GROGER) [33,34]. While the signaling pathways for GROGER- and GLOGER-motifs are not well described, GFOGER-containing peptides are known to activate Focal Adhesion Kinase (FAK) phosphorylation [35,36,37] and Akt signaling via PI3K [38]. The minimally functional GER motif has been linked to inhibition of angiogenesis [39,40]. Moreover, the PGP motif has been reported to interact with C-X-C chemokine receptor 1/2 (CXCR1/2) [41,42,43], activating pathways such as GRP-Rac1, p21-Activated Kinase (PAK) and Extracellular signal-regulated kinase (ERK) [44], while reducing Akt phosphorylation [45]. PGP also induces inflammatory responses, including neutrophil chemotaxis [41,42,43] and cytokine secretion [46], and promotes endothelial progenitor cell migration, proliferation and neovascularization [47]. Additionally, the RGD motif, which interacts with integrin α5β1 and integrin αvβ3 after collagen denaturation, has similar biological effects [48,49,50,51]. Comparable activities have been reported for matrikines derived from other types of collagen [52]. However, most studies on matrikines and collagen activity rely on short synthetic peptides at high concentrations, rather than on the naturally occurring collagen-derived peptides at physiological levels. Naturally occurring collagen-derived peptides have been associated with conditions such as kidney, cardiovascular and liver diseases, and have been proposed as biomarkers for these disorders [53,54,55,56]. These diseases often involve compromised endothelial function [57,58]. Understanding how abundant collagen peptides influence endothelial cells is therefore biologically relevant and may reveal mechanisms through which these peptides impact vascular health and disease progression.
Previous research using CE–MS/MS to analyze peptides in plasma and urine from 22 subjects highlighted a significantly higher abundance of COLα1 (I)-derived peptides in urine compared to plasma, with strong correlations observed among their relative abundances in both samples [59]. In contrast, non-collagen-derived peptides showed poor correlations between their urine and plasma abundances [59]. This discrepancy may be due to active reabsorption mechanisms in kidney tubules, which likely prevents most plasma peptides from being detectable in urine [60]. However, collagen-derived peptides may be exempt from this reabsorption process, potentially due to their potential biological activity.
Based on the above reports, we investigated whether abundant, COLα1 (I)-derived peptides [59] influence endothelial cell migration at physiological concentrations, as reported for full length COL(I) [7,15,61,62,63]. In a second step, potential molecular mechanisms for an observed impact on endothelial cells are investigated. The methodology and an overview of results are summarized in Figure 1.

2. Results

2.1. Shortlisting of Peptides Likely to Have Biological Activity Based on Abundance

We hypothesized that collagen-derived peptides are excluded from tubular reabsorption due to potential biological effects on endothelial cells. Accordingly, we assume that the more abundant a collagen-derived peptide is in urine, the more likely it is to have biological activity. Based on the findings of Magalhães et al. [59], we shortlisted nine highly abundant, non-overlapping COLα1 (I)-derived peptides identified in urine.
A closer examination of these peptide sequences (Table 1) revealed the prominent presence of the PGP motif previously reported to interact with cell-surface receptors [41,42,43,44,45,46,47,64], in seven out of the nine peptides. One of these peptides, in addition to the PGP motif [41,42,43,44,45,46,47,64], also contained the DGEA motif [21,22,23,24,25,26,27,28,29,30,31,32] and the GRPGER motif [33,39,40,65]). No known motifs were identified in the remaining two peptides. In line with previous analyses of the urinary peptidome of COLα1 (I) [66], the PGP motif, which appears 51 times in the mature COLα1 (I) sequence, was frequently detected, while peptides containing the DGEA or GRPGER motifs—appearing twice and once, respectively—in the mature COLα1 (I) [67] were much less frequent [66].

2.2. Inhibition of Collagen-Induced Migration by a Naturally Occurring Urinary Peptide

The selected naturally occurring COL(I)-derived peptides were tested at physiological concentrations (100 nM, as further explained in Section 4.2) in a migration assay using Human Umbilical Vein Endothelial Cells (HUVEC). Full-length COL(I) was applied as a control. COL(I) treatment doubled endothelial cell migration compared to the negative control (p-value < 0.001, mean number of migrated cells 30.58 for COL(I)-treated vs. 17.02 for negative control). Surprisingly, none of the nine peptides significantly affected endothelial migration (Supplementary Figure S1). Mean migrated cell counts and standard deviations for all treatments are reported in Appendix B, Table A1, a comparison of the number of migrated cells for peptide-treated cells is shown in Supplementary Figure S1, with representative microscope images in Supplementary Figure S2. However, one peptide (Peptide 1 (229NGDDGEAGKPGRPGERGPpGp249, containing the DGEA, PGP, and GRPGER motifs indicated in bold), significantly inhibited collagen-induced migration at physiological concentrations, reducing cell migration by 50% (p-value < 0.001; mean number of migrated cells 30.58 for COL(I)-treated vs. 15.92 for COL(I) + peptide 1-treated cells) (Figure 2). No effect on COL(I)-induced migration was observed for the other peptides at physiological concentrations.

2.3. Modeling Peptide 1 and Integrin α2β1 Receptor Interaction with AlphaFold 3

Flow cytometry analysis confirmed the expression of integrin α2β1 receptors on HUVEC (Figure 3A), confirming the proteomic identification of integrin α2 and β1 in all samples (Supplementary File, tab integrin receptors). To explore peptide–receptor interactions, AlphaFold 3 [68] was employed to model the interaction of Peptide 1 with integrin α2β1. The model consistently predicted proximity of Peptide 1 residues 242GER244 (GRPGER motif) with integrin α2 residues 154NS155, and Peptide 1 residue 245G with residue 219D of integrin α2 (Figure 3B). Integrin receptor positions were based on UniProtKB annotations after pro-peptide elimination [69,70].

2.4. Investigation of Potentially Affected Signaling Pathways

We explored signaling pathways reported to be activated by COL(I), and the effects of Peptide 1 at physiological concentrations. Initial Western blot analyses (Supplementary Figure S3) suggested activation of phospho-ERK, a known downstream effector of collagen I stimulation [6,7,8,9,10,11,12,13]); however, the results were not consistent, and the observed potential impact was low. Therefore, mass-spectrometry was employed to identify phospho-peptides in HUVEC treated with COL(I) alone or in combination with Peptide 1.
Twenty-one phospho-peptides were identified with high-confidence in the COL(I) or COL(I) + Peptide 1-treated samples (Figure 4A; Supplementary File, tab differential abundance testing). Six of these phospho-peptides exhibited significant differences in abundance between Peptide 1 + COL(I)-treated samples and COL(I)-treated samples (Figure 4A), five downregulated, one upregulated. Among these, four phospho-peptides (originating from CALU, CANX, HSPB1 and VIM) showed significantly decreased abundance in the Peptide 1 + COL(I)-treated samples as compared to the COL(I)-treated samples across at least two of the time points tested (7 min, 30 min, 60 min) (Figure 4B). Table 2 summarizes the phospho-peptides with significant differential abundance between the two tested conditions (Peptide 1 + COL(I) versus COL(I)).

3. Discussion

COLα1 (I)-derived peptides have been associated with conditions such as kidney, cardiovascular and liver diseases, and have been proposed as biomarkers for these disorders [53,54,55,56]. We investigated the hypothesis that urinary COLα1 (I)-derived peptides may exert biological effects on endothelial cells, based on prior findings [59] suggesting that these peptides, unlike those derived from other proteins, may not undergo active tubular reabsorption, of yet unknown mechanism [59]. In this study, we examined nine abundant, non-overlapping urinary COLα1 (I)-derived peptides identified in Magalhães et al. [59]. Among these, only one peptide, Peptide 1 (229NGDDGEAGKPGRPGERGPpGp249) demonstrated a significant effect in attenuating collagen-induced endothelial migration at a physiological concentration. Notably, Peptide 1 alone, like all other peptides tested, had no measurable effect at the tested concentration (Figure 2, Supplementary Figure S1). This peptide is one of the peptides part of biomarker panels for kidney, cardiovascular and liver diseases [53,54,55,56]. Interestingly, these diseases often involve compromised endothelial function [57,58], and more specifically, impaired endothelial cell migration [74,75].
Collagen-induced endothelial migration is well documented [61,63,76], and synthetic peptides containing sequences from COLα1 (I), such as DGEA [28,29], PGP [47], and GER-containing peptides [39,40], have been shown to influence migration. However, these synthetic peptides may not accurately represent the behavior of the naturally occurring peptides, as they frequently were used in much higher than the physiological concentration (even at micromolar levels), and/or only very short peptides encompassing these 3–6 amino acids were investigated, while, in general, much larger peptides, containing the respective motifs, are observed in vivo. To our knowledge, this study is the first to report a biological function for a naturally occurring urinary COLα1 (I)-derived peptide at physiological concentrations, representing an advancement beyond existing studies that used high concentration of non-naturally occurring peptides, and thus providing findings likely more reflective of physiological conditions. Other studies have highlighted the biological activity of naturally occurring peptides in plasma, for example, a study by Lindsey et al. [77] identified, through mass spectrometry, a naturally occurring COLα1 (I)-derived peptide in the plasma of myocardial infarction patients, which was generated by cleavage of the C-terminal region of COLα1 (I), 37 amino acids upstream of the collagen C-telopeptide. Lower levels of this peptide in the plasma were correlated with reduced cardiac function. The “activity peptide” (p1158/59, sequence AcRTGDSGPAGPPGPPG-NH2), enhanced fibroblast migration and wound closure rates in vitro, while in vivo, in mouse models, it improved cardiac function by attenuating increases in left ventricular end-diastolic and end-systolic diameters post- myocardial infarction. Despite its promising cardioprotective effects in a mouse model, the receptor, functional motif, and signaling pathways involved remain unidentified [77]. These findings, together with our results, highlight the therapeutic potential of naturally occurring peptides.

3.1. Biological Activity of Peptide 1 and Suggested Interaction with Integrin α2β1

Our findings suggest that Peptide 1, which uniquely contains DGEA and GRPGER motifs among the investigated peptides, may interact with the integrin α2β1 receptor. This receptor has been previously implicated in collagen interactions, particularly through motifs like GFOGER [33,34]. Previous research highlights PGP-induced endothelial cell migration [47]; nevertheless, it should be noted that the peptide used in these earlier experiments was acetylated. Furthermore, PGP acts through interaction with CXCR [41,42,43]. Considering HUVEC only have low expression of CXCR1 [78], this may explain the discrepancy with our results (i.e., no impact of PGP-containing peptides on migration), and underscores the need for further testing the biological impact of such peptides on multiple additional cell lines at physiological concentrations. We confirmed the presence of the integrin α2β1 receptor on HUVEC and used AlphaFold 3 to predict proximity between Peptide 1 and receptor residues. Predicted proximity included residues 242GER244 (part of the GRPGER motif) proximal to 154N [79,80], 155S [80], and residue 245G proximal to 219D [79,80] of integrin α2. These residues are critical for collagen binding to integrin α2β1 [81], as confirmed by site-directed mutagenesis and adhesion assays in human platelets [82]. It is important to note that, while AlphaFold 3 predictions support our hypothesis, direct experimental confirmation of Peptide 1 binding to integrin α2β1 is still required.

3.2. Investigation of Signaling Pathways

To understand the mechanisms underlying the effects of Peptide 1, we investigated its impact on signaling pathways activated by COL(I). Initial attempts using Western blot analysis failed to detect significant effects (i.e., significant differential phosphorylation), likely due to insufficient sensitivity for subtle changes. Moreover, while based on previous literature phospho-Akt and phospho-ERK are likely to be involved, based on the signaling pathways activated by the investigated motifs (cf. Introduction) [24,38,44,45], such studies frequently employed higher concentrations of regularly short peptides (shorter than the used naturally occurring peptides), which may alter the observed effects on cell signaling (e.g., an effect may be less pronounced or delayed). Therefore, we opted to employ mass spectrometry-based phospho-proteomics which has increased sensitivity and is an untargeted approach. The spectra of the suspected phospho-peptides were manually evaluated for credibility, leveraging respective previously published strict identification criteria [83,84]. Using this stringent analysis, six significantly affected phospho-peptides were successfully identified. Four of these phospho-peptides, previously associated with cell migration, were significantly increased in COL(I)-treated samples. This increase was abrogated in samples treated with both COL(I) and Peptide 1 (Supplementary File, tab differential abundance testing; Table 2).
The literature provided additional insights into these phospho-peptides and their potential roles in migration. Vimentin is phosphorylated at 56S by PAK1, which is required for integrin β1 activation. Inhibition of PAK1 reduces focal adhesion sites and cell extensions, while constitutively active PAK1 enhances them [85]. Heat Shock Protein Beta 1 (Hsp27), phosphorylated at 82S, prevents filament degeneration and promotes polymerization, facilitating cell migration [86,87]. Knockdown of Hsp27 or its upstream kinases inhibits VEGF-induced migration in HUVEC [88]. Calumenin, a target of Src kinase [89], is associated with increased cell migration and may act through ERK signaling pathways [90]. Notably, SRC kinase is activated by collagen binding to the integrin α2β1 [5,6]. Phosphorylated calnexin is involved in the folding of newly synthesized N-linked glycoproteins [91], which are increased in the cell membrane of migrating endothelial cells [92]. While these results were carefully examined (both evaluating the spectra and examining their relevance in the literature), and support the results of the migration assays, further validation and also studies investigating the exact signaling pathways triggered by the receptor and explaining the migration read-out are needed.

3.3. Proposed Model of Peptide 1 Action

A model summarizing our findings and the literature is presented in Figure 5. Briefly, COL(I) activates integrin β1 receptors, a process facilitated by phosphorylated vimentin [85]. This activation triggers downstream signaling, including Src kinase-mediated phosphorylation of calumenin, which supports migration [90]. Phosphorylated Hsp27 contributes to actin filament bundling and filopodia formation, essential for cell migration [86,88,93]. Phosphorylated calnexin aids in the folding of N-linked glycoproteins, which later move to the cell membrane and affect cell adhesion and migration [91,92].
Peptide 1 appears to reduce the respective phosphorylation and thereby inhibits activation of these pathways by COL(I) at a physiological concentration. A possible mechanistic explanation could be that Peptide 1, by binding to only one integrin α2β1 receptor as a monomer, prevents receptor crosslinking. Mature collagen, as a trimer, would typically crosslink two receptors and so initiate signaling [94]. However, the interaction of peptide 1 with integrin α2β1 and its proposed effect on the receptor crosslinking remains to be experimentally confirmed.

4. Materials and Methods

4.1. Cell Culture

HUVEC were chosen for their fidelity in modeling human endothelial cells and their sensitivity to extracellular matrix (ECM) changes [95]. Pooled HUVEC (Gibco—Thermo Fisher Scientific (Waltham, MA, USA), C0155C) were cultured at 37 °C with 5% CO2 under sterile conditions in Endothelial Growth Medium 2 (EGM2; Lonza (Basel, Switzerland), CC-3162), with 2% Fetal Bovine Serum (FBS) (Gibco—Thermo Fisher Scientific, 11570506) initially supplemented with 20% FBS until the first passage post-thaw. Thereafter, cells were maintained in EGM2 supplemented with 10% FBS. Experiments were conducted using cells at passage 5 or 6, with the same passage used for biological replicates. Experimental conditions for each assay are described in their respective sections. All cells tested negative for Mycoplasma contamination and were confirmed positive for Cluster of Differentiation 31 (CD31) using immunohistochemistry.

4.2. Peptides and Collagen Type I

Physiological concentration for the peptides was calculated as follows: in a previous study, we assessed the physiological concentrations of the COL1 peptides based on the analysis of a standard urine sample representing normal urine from healthy individuals [96]. This information, together with the average relative abundance of these peptides measured by mass spectrometry in 555 samples from individuals in the general population, was used to estimate the physiological concentrations of the COL1 peptides investigated in this study. For the nine peptides analyzed, the average concentration ranged from 14 nM to 95 nM. Specifically, for matrikine 1, the 95% confidence interval was 15.6 to 127.7 nM. The distribution of matrikine 1 concentrations in the 555 general population samples is presented in Supplementary Figure S5. As the concentration ranges varied across peptides, we tested a uniform concentration of 100 nM for all, which falls within the range of physiological concentrations.
Urinary collagen-derived peptides (sequences listed in Table 1, based on [59]), were purchased from Genosphere Biotechnologies (Clamart, France). Peptides were resuspended in cell culture grade water and used at a final concentration of 100 nM in Phosphate-Buffered Saline (PBS). Human Collagen Type I (COL(I); Sigma (St. Louis, MO, USA), C7774) was solubilized in 0.5 M acetic acid at a concentration of 0.33 mg/mL used at a final concentration of 10 nM (1.4 μg/mL) in PBS.

4.3. Migration Assay

HUVEC were serum-starved in a 10:1 mix of Endothelial Basal Medium (EBM2; Lonza, CC-3156) and EGM2 (final FBS concentration: 0.2%) for 24 h before the experiment. Cells were harvested with trypsin-Ethylenediaminetetraacetic acid (EDTA; Gibco, 15400054) and seeded into transwell permeable supports (Costar (Berlin, Germany), 3421) with a 5.0 μm pore polycarbonate membranes at a density at 15,000 cells per insert. Treatments listed in Appendix B, Table A1 were added to the lower wells (100 nM peptides and 10 nM COL(I), or the same volume of acetic acid dilution in PBS as was added for COL(I)). Migration occurred over 16 h [97], after which medium and non-migrated cells were removed. Migrated cells were fixed in 4% paraformaldehyde (PFA) and stained with haematoxylin and eosin. Five representative images per insert were captured at 10× magnification (LEICA DMIRE2 microscope (Wetzlar, Germany); LAS X software version 3.9.1) and analyzed using ImageJ’s (v2.14) cell counter plugin. Inserts showing damages were excluded from the analysis. Repeat experiments were conducted for treatments with initial promising results (COL(I), Peptide 1 + COL(I) and Peptide 1). Biological replicates are shown in Appendix B, Table A1, and each biological replicate has five technical replicates. Data analysis utilized a negative binomial generalized linear model, followed by post hoc Welch two-sample t-tests (p < 0.05).

4.4. Flow Cytometry Analysis

Medium was removed and cells were washed with PBS and harvested using 0.05% EDTA (Gibco, 15576028). After centrifugation (200 g), cells were resuspended in growth medium, washed again, and centrifuged at 360 g, followed by washing in PBS + 0.5% Bovine Serum Albumin (BSA; PanReacAppliChem (Milan, Italy), A1391). For each FACS tube, 5 × 105 cells were prepared. Cells were incubated with Fc block (Miltenyi Biotec (Athens, Greece), 130-059-901) for 10 min at room temperature to reduce nonspecific binding, then stained with primary antibodies for integrin α2 (CD49b—FITC–conjugated; BD Biosciences (Athens, Greece) Cat# 555498, RRID:AB_395888) and integrin β1 (CD29; BD Biosciences Cat# 556048, RRID:AB_396319)) in PBS + 0.5% BSA for 1 h at 37 °C in the dark. Cells were washed using PBS + 0.5% BSA, and cells stained with integrin β1 were treated with a secondary antibody (Rabbit Anti-Mouse IgG, RPE-conjugated; Agilent Cat# R043901, RRID:AB_579537), and washed as before. All cells were incubated with 7-Aminoactinomycin D (7-AAD, BioLegend (Athens, Greece), 640922) for live-dead staining. Calibration and quality control of the BD FACS Celesta instrument were performed before analysis. Data was analyzed using FCS Express v7.24.0024 (https://denovosoftware.com/ (accessed on 8 June 2025)), selecting the cell population, gating out dead cells and comparing double-positive stained cells to isotype controls.

4.5. Alphafold 3 Predictions

Integrin α2 and β1 amino acid sequences were obtained from UniProtKB, and propeptides were removed [69,70]. Peptide 1 sequence is provided in Table 1. AlphaFold 3 webserver [68] predictions of integrin–peptide interactions were performed with hydroxyprolines specified for Peptide 1. Predictions (25 models across five runs using different seed numbers) identified residues in Peptide in proximity with integrins (≥0.5 interaction probability) in at least 20/25 models. Data-analysis was conducted in R v4.4.0. Further model visualization was performed using Mol* Viewer (https://molstar.org/viewer/ (accessed on 8 June 2025)) version 4.18.

4.6. Sample Collection

Cells were seeded in 6-well plates (50,000 cells/well) and cultured in EGM2 + 10% FBS for 2 days to ~80% confluency, then starved for 16 h. Treatments (Peptide 1, COL(I), Peptide 1 + COL(I) or vehicle controls) were applied at migration assay concentrations. Positive controls included 5% FBS and 1 mM hydrogen peroxide. Untreated cells received no additions. After 7, 30, or 60 min of treatment, plates were placed on ice, washed with ice-cold PBS, and lysed in Laemmli buffer (1×, with 3.6% protease inhibitor, 50 mM sodium fluoride, and 100 μM activated sodium orthovanadate), and cell lysate was collected using cell scrapers. Lysates were bath sonicated for 10 min and stored at −20 °C. Two biological and two technical replicates were collected (four replicates in total).

4.7. Western Blotting

Western blotting was performed as described [98,99] with modifications. Cell extracts (20 μL) were loaded onto polyacrylamide gels until maximum separation was achieved at 40–75 kDa, and proteins transferred to nitrocellulose membranes (Amersham™ Protran® Athens, Greece), GE10600004) for 2 h on ice. Membranes were blocked in 5% milk-TBS + 0.1% Tween™ 20 for 2 h, washed with TBS-0.1% Tween™ 20, and incubated overnight at 4 °C with primary antibodies (phospho-ERK, Cell Signaling (Athens, Greece), Cat# 9106; RRID:AB_331768); ERK, Cell Signaling, Cat# 9107, RRID:AB_10695739; phospho-Akt, Cell Signaling, Cat# 4060, RRID:AB_2315049; 1:1000). Secondary antibodies (1:2000 dilution for both antibodies, Goat Anti-Mouse IgG, HRP; Cell Signaling, Cat# 91196, RRID:AB_2940774, or Goat Anti-Rabbit IgG, HRP; Sigma, Cat# AP132P, RRID:AB_90264; 1:2000) were applied for 2 h at room temperature. Detection used enhanced chemiluminescence (Perkin and Elmer (Athens, Greece), NEL104001EA) and Super RX-N films (Fujifilm (Dublin, Ireland), 47410 19289). Signals were quantified with Quantity One (Bio-Rad (version 4.6.8)). Phosphoprotein signals were normalized to total protein (ERK) or Coomassie-stained gels (Akt). Analytical gels were run in parallel with their counterparts for Western blot, but instead of proceeding to transfer, were incubated with Coomassie brilliant blue overnight and analyzed with Quantity One.

4.8. LC–MS/MS Analysis and MS Data Processing

Protein extracts (20 μL) were resolved on 12% polyacrylamide gels (5% stacking,) for GeLC–MS/MS [100]. After concentrating the samples in the separating gel using electrophoresis, gels were washed and protein bands were visualized with Coomassie stain, excised, destained (40% acetonitrile, 50 mM ammonium bicarbonate), reduced (10 nM Dithioerythritol in 100 mM ammonium bicarbonate) and alkylated (10 mg/mL iodoacetamide in 100 mM ammonium bicarbonate). Gel pieces were washed with 100 mM ammonium bicarbonate, destain solution and ultrapure water subsequently (each for 20 min at room temperature, while shaking) and dried under vacuum, and trypsinised overnight using 600 ng trypsin per sample in 10 mM ammonium bicarbonate. Peptides were extracted with subsequent incubations in 50 mM ammonium bicarbonate, and twice with 10% formic acid 1:1 with acetonitrile; all three for 15 min at room temperature while shaking. Peptide solutions were cleared with a fresh PVDF filter (Merck Milipore, (Athens, Greece)) for each sample, and peptides were dried under vacuum. Peptides were reconstituted in mobile phase A (98% water, 2% acetonitrile, 0.1% formic acid) and analyzed using a Dionex Ultimate 3000 HPLC nanoflow system coupled to a Q Exactive™ Plus Orbitrap™ mass spectrometer (Thermo Finnigan, Bremen, Germany). Samples (10 μL) were loaded onto a Dionex 0.1 × 20 mm, C 18 trap column (flow rate: 5 μL/min) and separated on an Acclaim PepMapTM C18 100 column spectrometer (Thermo Fischer Scientific, Bremen, Germany) (75 μm × 50 cm, flow rate 300 nL/min). A 250 min gradient (2–95% (mobile phase B: 80% acetonitrile, 0.1% formic acid) was applied. Positive ionization was performed at 2.1 kV. Data were collected in MS/MS Data Dependent Acquisition mode. Raw files were analyzed in Thermo Proteome Discoverer 2.4 using the Sequest search engine and the UniProtKB Homo sapiens database (canonical sequences, downloaded 20 June 2019, 20,431 reviewed entries). Carbamidomethylation (cysteine) was a static modification; methionine oxidation and phosphorylation (serine, threonine, tyrosine) were dynamic. Peptide data was filtered (FDR q-value= 0.01; maximum peptide rank = 1). Label-free quantification employed precursor ion peak area. Normalized data were log2-transformed. Supplementary File, tab normalized abundance data, provides normalized abundance data. More details on LC–MS/MS and MS data settings are provided in Appendix A.1.

4.9. Statistical Analysis of Proteomics Results

Phospho-peptides were filtered to retain credible data: peptides with one Peptide-Spectral Match (PSM), lacking unphosphorylated counterparts or with poor spectra were excluded (Supplementary File, tab removed peptides, Supplementary Figure S4). Spectra were manually inspected based on defined criteria [83,84]. Retained peptides fulfilled ≥3/4 criteria. Details are in Supplementary File, tab spectra analysis. Peptide and COL(I)-treated cells (n = 4/time point) were compared to COL(I)-only treated cells. Time-independent comparisons applied Benjamini–Hochberg (BH) correction to p-values. Wilcoxon rank sum tests evaluated differential abundance. Analyses were performed in R v4.4.0. More details on peptide filtering are provided in Appendix A.2.

5. Conclusions

Our results demonstrate that a naturally occurring COLα1 (I)-derived peptide can inhibit collagen-induced endothelial migration at a physiological concentration, probably by interacting with the integrin α2β1 receptor. It should be noted that the interaction of peptide 1 with this receptor, while supported by the AlphaFold 3 predictions and phospho-proteomics results, has not yet been experimentally confirmed. In addition, key signaling molecules involved in migration are downregulated in COLα1 (I) + Peptide 1 treated cells, when compared to COLα1 (I) alone, further supporting the classification of Peptide 1 as a matrikine. However, further investigation to elucidate the exact underlying signaling mechanism is needed.
These findings open new avenues for research into the detailed characterization of Peptide 1 mechanism of action and its therapeutic potential. Future studies should also explore the mechanisms underlying its urinary excretion, which may be linked to its biological activity, which would aid in understanding the peptide’s role as a biomarker in diseases associated with endothelial dysfunction.

Supplementary Materials

The supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms26157480/s1.

Author Contributions

Conceptualization, I.K.M., M.M. (Marika Mokou), J.Z., H.M., A.V. and A.L.; Data curation, H.D.; Formal analysis, H.D., J.Z. and M.G.R.; Funding acquisition, H.M., A.V. and A.L.; Investigation, H.D., I.K.M., F.P., M.M. (Manousos Makridakis), A.T., A.L. and M.G.R.; Methodology, I.K.M., F.P., M.M. (Manousos Makridakis), A.T., M.M. (Marika Mokou), J.Z., H.M., A.V., A.L. and M.G.R.; Project administration, A.V. and A.L.; Resources, A.V. and M.G.R.; Supervision, J.Z., H.M., A.V., A.L. and M.G.R.; Validation, H.D., I.K.M. and A.V.; Visualization, H.D.; Writing—original draft, H.D.; Writing—review and editing, H.D., M.M. (Marika Mokou), J.Z., H.M., A.V., A.L. and M.G.R. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by European Union’s Horizon Europe Marie Skłodowska-Curie Actions Doctoral Networks—Industrial Doctorates Program (HORIZON—MSCA—2021—DN-ID), grant number 101072828. Funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union. Neither the European Union nor the granting authority can be held responsible for them.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Raw proteomics data files have been deposited on MassIVE (identifier: MSV000097296; username for reviewer access: MSV000097296_reviewer; password: dV5qsfE4rYv37q81) as well as ProteomExchange (PXD061753) and Supplementary File. A ProteomExchange data set identifier has been requested. The experimental metadata file has been generated using lesSDRF [101]. Supplementary File is similarly available through MassIVE under the same identifier.

Acknowledgments

Figure 1 and Figure 5 were made with the aid of Servier Medical Art (CC BY 4.0 license).

Conflicts of Interest

Mischak, H. is the founder and co-owner of Mosaiques Diagnostics GmbH (Hannover, Germany). Mina, I.K., Latosinska, A. and Mokou, M., are employees of Mosaiques Diagnostics GmbH. Devos, H. is currently employed by Nordic Bioscience A/S. The remaining authors declare no conflicts of interest. The funding body was not involved in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.

Abbreviations

The following abbreviations are used in this manuscript:
7-AAD7-Aminoactinomycin D
BSABovine Serum Albumin
CE–MS/MScapillary electrophoresis combined with mass spectrometry
CKDChronic kidney disease
COL(I)Collagen type I
COLα1 (I)Collagen type I, alpha 1 chain
COLα2 (I)Collagen type I, alpha 2 chain
COPDchronic obstructive pulmonary disease
CXCLC-X-C Ligand
CXCRC-X-C Chemokine receptor
EBM2Endothelial Basal Medium
ECMExtracellular Matrix
EDTAEthylenediaminetetraacetic acid
EGM2Endothelial Growth Medium
ERKExtracellular Regulated Kinase
FAKFocal Adhesion Kinase
FBSFetal Bovine Serum
FDRFalse Discovery Rate
FITCFluorescein isothiocyanate
HUVECHuman Umbilical Vein Endothelial Cells
IP31,4,5-triphosphate
JNKC-jun N-terminal kinase
LC–MS/MSLiquid Chromatography—Mass spectrometry
MMPMatrix-metalloproteinases
PAKP21 (Rac1) Activated Kinase
PEphycoerythrin
PFAParaformaldehyde
PBSPhosphate-Buffered Saline
PKCProtein Kinase C
PLCPhospholipase C
PRAKP38-regulated/activated protein kinase
PSMPeptide-spectral match
STATSignal Transducer and Activator of Transcription
TBSTris-buffered Saline
VEGFVascular Endothelial Growth Factor

Appendix A

Appendix A.1

For LC–MS/MS analysis a Dionex Ultimate 3000 HPLC nanoflow system was used in combination with a Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer. Samples were reconstituted in 25 μL mobile phase A (98% water, 2% acetonitrile, 0,1% formic acid) and 10 μL was loaded onto the LC column configured with a Dionex 0.1 × 20 mm, 5 μm, 100 Ä C 18 nano trap column. A flow rate of 5 μL/min was used. An Acclaim PepMapTM C18 100 nanoViper column 75 μm × 50 cm, 2 μm 100 Ä with a flow rate of 300nL/min was used as analytical column. The column was washed and re-equilibrated prior to each sample injection. Mobile phase B consisted of 0.1% formic acid in 80% acetonitrile. The eluent was ionized using a nanospray FlexTM ESI source in positive ion mode, whereas the Q Exactive Orbitrap (Thermo Finnigan, Bremen, Germany), was operated in MS/MS mode. Peptides were eluted under a 250 min gradient from 2% mobile phase B to 95% mobile phase B. Positive ion electrospray ionization with a voltage of 2.1 kV was used for gaseous phase transition of eluted, separated peptides. The mass spectrometer parameters were set as follows: (1) full MS: resolution of 70,000, automatic gain control (AGC) target at 1e6, maximum injection time (IT) of 100 ms, scan range of 380–1200 m/z; (2) dd-MS2: resolution of 17,00, AGC target at 1*105, maximum IT of 50 ms, TopN of 20, normalized collision energy of 30; (3) dd settings: intensity threshold 1.6*105, Minimum AGC target 8.00*103, dynamic exclusion for 15 s.
Raw files were processed with Thermo Proteome Discoverer 2.4 software, utilizing the Sequest search engine and the UniProt Homo sapiens fasta database containing only canonical sequences (downloaded on 20 June 2019, including 20,431 reviewed entries). Carbamidomethylation of cysteine was set as a static modification, and oxidation of methionine and phosphorylation of serine, threonine and tyrosine were set as dynamic modifications. Two missed cleavage sites, a precursor mass tolerance of 5 ppm, and fragment mass tolerance of 0.05 Da were allowed. Feature mapper node was run with default settings. The following filters were also used: peptide: High Confidence (FDR q value based = 0.01), peptide rank: Maximum rank = 1, peptide grouping: enabled, protein grouping: enabled. For quantification, the label-free method was employed using the peak area of precursor ions to determine the relative abundance of identified proteins. the label-free method was employed using the peak area of precursor ions to determine the relative abundance of identified proteins and peptides. Missing values were replaced by zeros. Part per million (ppm) normalization was applied once at the protein level to evaluate the presence of the integrin receptor and once at the peptide level to evaluate phosphorylation events, by dividing the peptide summed peak area by the total peak area in a sample, multiplied by one million. Normalized abundance data was then log2-transformed, and, for phospho-peptides, can be found in Supplementary File, tab “normalized abundance data”.

Appendix A.2

Phosphorylated peptides’ credibility was assessed as follows: first, all peptides with only one Peptide-Spectral Match (PSM) were discarded (n = 21), peptides without unphosphorylated peptide mapping to the same protein, were removed (n = 6), and peptides with poor spectra (n = 7) were filtered out (Supplementary File, tab “removed peptides”; mass spectra can be found in Supplementary Figure S2). The criteria for the manual inspection of the spectra were: (a) four sequential, explained fragments had to be observed or five explained fragments, separated by maximum one unexplained fragment, in a series of six sequential fragments had to be observed; (b) the b- or y-ion corresponding to the breakage of the proline peptide bond had to have an intensity of at least 50% of the most intense ion observed in the spectrum; (c) at least six out of the ten most intense ions had to correspond to b- or y-ions; (d) at least three ions corresponding to the neutral loss of the phospho-group had to be observed. For the last rule, an exception was made if the peptide contained multiple prolines, as this has been shown to affect the neutral loss of the phospho-group [84]. Peptides of which the spectra fulfill at least three out of four criteria, based on the work of Potel et al. [83], were retained.

Appendix B

Table A1. Overview of the mean number of migrated cells and their standard deviation, for each of the peptides, their combination with full-length COL(I), COL(I) by itself and negative control. The number of replicates (biological and technical) is additionally reported. Lastly, the number of independent experiment (experiments conducted on different days, using cells of the same passage number) has additionally been provided.
Table A1. Overview of the mean number of migrated cells and their standard deviation, for each of the peptides, their combination with full-length COL(I), COL(I) by itself and negative control. The number of replicates (biological and technical) is additionally reported. Lastly, the number of independent experiment (experiments conducted on different days, using cells of the same passage number) has additionally been provided.
TreatmentMean Number of Migrated CellsStandard DeviationNumber of ReplicatesNumber of Independent Experiments
Peptide 118.74.3152
Peptide 220.57.14101
Peptide 314.63.78101
Peptide 418.94.25101
Peptide 5205.08101
Peptide 614.25.55101
Peptide 716.97.75101
Peptide 816.94.72101
Peptide 916.34.83101
Negative control17.025.93557
Collagen type I30.587.41607
Peptide 1 + collagen type I15.924.44252
Peptide 2 + collagen type I27.737.73152
Peptide 3 + collagen type I27.88.66152
Peptide 4 + collagen type I31.537.53152
Peptide 5 + collagen type I30.873.48152
Peptide 6 + collagen type I28.138.71152
Peptide 7 + collagen type I31.1316.75152
Peptide 8 + collagen type I29.276152
Peptide 9 + collagen type I317.72152

References

  1. Theocharis, A.D.; Manou, D.; Karamanos, N.K. The Extracellular Matrix as a Multitasking Player in Disease. FEBS J. 2019, 286, 2830–2869. [Google Scholar] [CrossRef] [PubMed]
  2. Devos, H.; Zoidakis, J.; Roubelakis, M.G.; Latosinska, A.; Vlahou, A. Reviewing the Regulators of COL1A1. Int. J. Mol. Sci. 2023, 24, 10004. [Google Scholar] [CrossRef] [PubMed]
  3. Shi, R.; Zhang, Z.; Zhu, A.; Xiong, X.; Zhang, J.; Xu, J.; Sy, M.-S.; Li, C. Targeting Type I Collagen for Cancer Treatment. Int. J. Cancer 2022, 151, 665–683. [Google Scholar] [CrossRef]
  4. Di Lullo, G.A.; Sweeney, S.M.; Korkko, J.; Ala-Kokko, L.; San Antonio, J.D. Mapping the Ligand-Binding Sites and Disease-Associated Mutations on the Most Abundant Protein in the Human, Type I Collagen. J. Biol. Chem. 2002, 277, 4223–4231. [Google Scholar] [CrossRef]
  5. Li, S.; Sampson, C.; Liu, C.; Piao, H.; Liu, H.-X. Integrin Signaling in Cancer: Bidirectional Mechanisms and Therapeutic Opportunities. Cell Commun. Signal. 2023, 21, 266. [Google Scholar] [CrossRef]
  6. Sun, L.; Guo, S.; Xie, Y.; Yao, Y. The Characteristics and the Multiple Functions of Integrin Β1 in Human Cancers. J. Transl. Med. 2023, 21, 787. [Google Scholar] [CrossRef] [PubMed]
  7. Aman, J.; Margadant, C. Integrin-Dependent Cell–Matrix Adhesion in Endothelial Health and Disease. Circ. Res. 2023, 132, 355–378. [Google Scholar] [CrossRef]
  8. Godoy, P.; Hengstler, J.G.; Ilkavets, I.; Meyer, C.; Bachmann, A.; Müller, A.; Tuschl, G.; Mueller, S.O.; Dooley, S. Extracellular Matrix Modulates Sensitivity of Hepatocytes to Fibroblastoid Dedifferentiation and Transforming Growth Factor Beta-Induced Apoptosis. Hepatology 2009, 49, 2031–2043. [Google Scholar] [CrossRef]
  9. Viale-Bouroncle, S.; Gosau, M.; Morsczeck, C. Collagen I Induces the Expression of Alkaline Phosphatase and Osteopontin via Independent Activations of FAK and ERK Signalling Pathways. Arch. Oral Biol. 2014, 59, 1249–1255. [Google Scholar] [CrossRef]
  10. Krasny, L.; Shimony, N.; Tzukert, K.; Gorodetsky, R.; Lecht, S.; Nettelbeck, D.M.; Haviv, Y.S. An In-Vitro Tumour Microenvironment Model Using Adhesion to Type I Collagen Reveals Akt-Dependent Radiation Resistance in Renal Cancer Cells. Nephrol. Dial. Transplant. 2010, 25, 373–380. [Google Scholar] [CrossRef]
  11. Jarvis, G.E.; Best, D.; Watson, S.P. Glycoprotein VI/Fc Receptor γ Chain-Independent Tyrosine Phosphorylation and Activation of Murine Platelets by Collagen. Biochem. J. 2004, 383, 581–588. [Google Scholar] [CrossRef] [PubMed]
  12. Takeuchi, Y.; Suzawa, M.; Kikuchi, T.; Nishida, E.; Fujita, T.; Matsumoto, T. Differentiation and Transforming Growth Factor-β Receptor Down-Regulation by Collagen-A2β1 Integrin Interaction Is Mediated by Focal Adhesion Kinase and Its Downstream Signals in Murine Osteoblastic Cells. J. Biol. Chem. 1997, 272, 29309–29316. [Google Scholar] [CrossRef] [PubMed]
  13. Ziaee, S.; Chung, L.W. Induction of Integrin A2 in a Highly Bone Metastatic Human Prostate Cancer Cell Line: Roles of RANKL and AR under Three-Dimensional Suspension Culture. Mol. Cancer 2014, 13, 208. [Google Scholar] [CrossRef]
  14. Stejskalová, A.; Fincke, V.; Nowak, M.; Schmidt, Y.; Borrmann, K.; von Wahlde, M.-K.; Schäfer, S.D.; Kiesel, L.; Greve, B.; Götte, M. Collagen I Triggers Directional Migration, Invasion and Matrix Remodeling of Stroma Cells in a 3D Spheroid Model of Endometriosis. Sci. Rep. 2021, 11, 4115. [Google Scholar] [CrossRef] [PubMed]
  15. Goetsch, K.P.; Kallmeyer, K.; Niesler, C.U. Decorin Modulates Collagen I-Stimulated, but Not Fibronectin-Stimulated, Migration of C2C12 Myoblasts. Matrix Biol. 2011, 30, 109–117. [Google Scholar] [CrossRef]
  16. Jabłońska-Trypuć, A.; Matejczyk, M.; Rosochacki, S. Matrix Metalloproteinases (MMPs), the Main Extracellular Matrix (ECM) Enzymes in Collagen Degradation, as a Target for Anticancer Drugs. J. Enzyme Inhib. Med. Chem. 2016, 31, 177–183. [Google Scholar] [CrossRef] [PubMed]
  17. Vizovišek, M.; Fonović, M.; Turk, B. Cysteine Cathepsins in Extracellular Matrix Remodeling: Extracellular Matrix Degradation and Beyond. Matrix Biol. 2019, 75, 141–159. [Google Scholar] [CrossRef]
  18. Jariwala, N.; Ozols, M.; Bell, M.; Bradley, E.; Gilmore, A.; Debelle, L.; Sherratt, M.J. Matrikines as Mediators of Tissue Remodelling. Adv. Drug Deliv. Rev. 2022, 185, 114240. [Google Scholar] [CrossRef]
  19. Latosinska, A.; Siwy, J.; Faguer, S.; Beige, J.; Mischak, H.; Schanstra, J.P. Value of Urine Peptides in Assessing Kidney and Cardiovascular Disease. Proteom. —Clin. Appl. 2021, 15, 2000027. [Google Scholar] [CrossRef]
  20. Latosinska, A.; Siwy, J.; Mischak, H.; Frantzi, M. Peptidomics and Proteomics Based on CE-MS as a Robust Tool in Clinical Application: The Past, the Present, and the Future. Electrophoresis 2019, 40, 2294–2308. [Google Scholar] [CrossRef]
  21. Staatz, W.D.; Fok, K.F.; Zutter, M.M.; Adams, S.P.; Rodriguez, B.A.; Santoro, S.A. Identification of a Tetrapeptide Recognition Sequence for the Alpha 2 Beta 1 Integrin in Collagen. J. Biol. Chem. 1991, 266, 7363–7367. [Google Scholar] [CrossRef]
  22. Cha, B.-H.; Shin, S.R.; Leijten, J.; Li, Y.-C.; Singh, S.; Liu, J.C.; Annabi, N.; Abdi, R.; Dokmeci, M.R.; Vrana, N.E.; et al. Integrin-Mediated Interactions Control Macrophage Polarization in 3D Hydrogels. Adv. Healthc. Mater. 2017, 6, 1700289. [Google Scholar] [CrossRef]
  23. Mehta, M.; Madl, C.M.; Lee, S.; Duda, G.N.; Mooney, D.J. The Collagen I Mimetic Peptide DGEA Enhances an Osteogenic Phenotype in Mesenchymal Stem Cells When Presented from Cell-Encapsulating Hydrogels. J. Biomed. Mater. Res. A 2015, 103, 3516–3525. [Google Scholar] [CrossRef]
  24. Madamanchi, A.; Santoro, S.A.; Zutter, M.M. A2β1 Integrin. Adv. Exp. Med. Biol. 2014, 819, 41–60. [Google Scholar] [CrossRef]
  25. Mineur, P.; Guignandon, A.; Lambert, C.A.; Amblard, M.; Lapière, C.M.; Nusgens, B.V. RGDS and DGEA-Induced [Ca2+]i Signalling in Human Dermal Fibroblasts. Biochim. Biophys. Acta BBA-Mol. Cell Res. 2005, 1746, 28–37. [Google Scholar] [CrossRef]
  26. McCann, T.J.; Terranova, G.; Keyte, J.W.; Papaioannou, S.S.; Mason, W.T.; Meikle, M.C.; McDonald, F. An Analysis of Ca2+ Release by DGEA: Mobilization of Two Functionally Distinct Internal Stores in Saos-2 Cells. Am. J. Physiol. Cell Physiol. 1998, 275, C33–C41. [Google Scholar] [CrossRef]
  27. McCann, T.J.; Mason, W.T.; Meikle, M.C.; McDonald, F. A Collagen Peptide Motif Activates Tyrosine Kinase-Dependent Calcium Signalling Pathways in Human Osteoblast-like Cells. Matrix Biol. 1997, 16, 273–283. [Google Scholar] [CrossRef]
  28. Kim, H.-K.; Joe, Y.A. DGDA, a Local Sequence of the Kringle 2 Domain, Is a Functional Motif of the Tissue-Type Plasminogen Activator’s Antiangiogenic Kringle Domain. Biochem. Biophys. Res. Commun. 2010, 391, 166–169. [Google Scholar] [CrossRef] [PubMed]
  29. Chen, S.; Chakrabarti, R.; Keats, E.C.; Chen, M.; Chakrabarti, S.; Khan, Z.A. Regulation of Vascular Endothelial Growth Factor Expression by Extra Domain B Segment of Fibronectin in Endothelial Cells. Investig. Ophthalmol. Vis. Sci. 2012, 53, 8333–8343. [Google Scholar] [CrossRef] [PubMed]
  30. Jha, A.; Moore, E. Collagen-Derived Peptide, DGEA, Inhibits pro-Inflammatory Macrophages in Biofunctional Hydrogels. J. Mater. Res. 2022, 37, 77–87. [Google Scholar] [CrossRef] [PubMed]
  31. Anderson, J.M.; Vines, J.B.; Patterson, J.L.; Chen, H.; Javed, A.; Jun, H.-W. Osteogenic Differentiation of Human Mesenchymal Stem Cells Synergistically Enhanced by Biomimetic Peptide Amphiphiles Combined with Conditioned Medium. Acta Biomater. 2011, 7, 675–682. [Google Scholar] [CrossRef]
  32. Chen, G.; Kong, P.; Jiang, A.; Wang, X.; Sun, Y.; Yu, T.; Chi, H.; Song, C.; Zhang, H.; Subedi, D.; et al. A Modular Programmed Biphasic Dual-Delivery System on 3D Ceramic Scaffolds for Osteogenesis in Vitro and in Vivo. J. Mater. Chem. B 2020, 8, 9697–9717. [Google Scholar] [CrossRef]
  33. Hamaia, S.; Farndale, R.W. Integrin Recognition Motifs in the Human Collagens. Adv. Exp. Med. Biol. 2014, 819, 127–142. [Google Scholar] [CrossRef]
  34. Knight, C.G.; Morton, L.F.; Peachey, A.R.; Tuckwell, D.S.; Farndale, R.W.; Barnes, M.J. The Collagen-Binding A-Domains of Integrins A1β1 and α2β1Recognize the Same Specific Amino Acid Sequence, GFOGER, in Native (Triple-Helical) Collagens. J. Biol. Chem. 2000, 275, 35–40. [Google Scholar] [CrossRef]
  35. Reyes, C.D.; García, A.J. A2β1 Integrin-Specific Collagen-Mimetic Surfaces Supporting Osteoblastic Differentiation. J. Biomed. Mater. Res. A 2004, 69A, 591–600. [Google Scholar] [CrossRef]
  36. Fraser, D.; Benoit, D. Dual Peptide-Functionalized Hydrogels Differentially Control Periodontal Cell Function and Promote Tissue Regeneration. Biomater. Adv. 2022, 141, 213093. [Google Scholar] [CrossRef]
  37. Clark, A.Y.; Martin, K.E.; García, J.R.; Johnson, C.T.; Theriault, H.S.; Han, W.M.; Zhou, D.W.; Botchwey, E.A.; García, A.J. Integrin-Specific Hydrogels Modulate Transplanted Human Bone Marrow-Derived Mesenchymal Stem Cell Survival, Engraftment, and Reparative Activities. Nat. Commun. 2020, 11, 114. [Google Scholar] [CrossRef]
  38. Consonni, A.; Cipolla, L.; Guidetti, G.; Canobbio, I.; Ciraolo, E.; Hirsch, E.; Falasca, M.; Okigaki, M.; Balduini, C.; Torti, M. Role and Regulation of Phosphatidylinositol 3-Kinase β in Platelet Integrin A2β1 Signaling. Blood 2012, 119, 847–856. [Google Scholar] [CrossRef] [PubMed]
  39. Sweeney, S.M.; DiLullo, G.; Slater, S.J.; Martinez, J.; Iozzo, R.V.; Lauer-Fields, J.L.; Fields, G.B.; Antonio, J.D.S. Angiogenesis in Collagen I Requires A2β1 Ligation of a GFP*GER Sequence and Possibly P38 MAPK Activation and Focal Adhesion Disassembly. J. Biol. Chem. 2003, 278, 30516–30524. [Google Scholar] [CrossRef] [PubMed]
  40. Taubenberger, A.V.; Bray, L.J.; Haller, B.; Shaposhnykov, A.; Binner, M.; Freudenberg, U.; Guck, J.; Werner, C. 3D Extracellular Matrix Interactions Modulate Tumour Cell Growth, Invasion and Angiogenesis in Engineered Tumour Microenvironments. Acta Biomater. 2016, 36, 73–85. [Google Scholar] [CrossRef] [PubMed]
  41. Patel, D.F.; Snelgrove, R.J. The Multifaceted Roles of the Matrikine Pro-Gly-Pro in Pulmonary Health and Disease. Eur. Respir. Rev. 2018, 27, 180017. [Google Scholar] [CrossRef]
  42. Misiura, M.; Miltyk, W. Proline—Containing Peptides-New Insight and Implications: A Review. BioFactors 2019, 45, 857–866. [Google Scholar] [CrossRef]
  43. Weathington, N.M.; van Houwelingen, A.H.; Noerager, B.D.; Jackson, P.L.; Kraneveld, A.D.; Galin, F.S.; Folkerts, G.; Nijkamp, F.P.; Blalock, J.E. A Novel Peptide CXCR Ligand Derived from Extracellular Matrix Degradation during Airway Inflammation. Nat. Med. 2006, 12, 317–323. [Google Scholar] [CrossRef]
  44. Hahn, C.S.; Scott, D.W.; Xu, X.; Roda, M.A.; Payne, G.A.; Wells, J.M.; Viera, L.; Winstead, C.J.; Bratcher, P.; Sparidans, R.W.; et al. The Matrikine N-α-PGP Couples Extracellular Matrix Fragmentation to Endothelial Permeability. Sci. Adv. 2015, 1, e1500175. [Google Scholar] [CrossRef]
  45. Li, X.; Tang, Y.; Yu, F.; Sun, Y.; Huang, F.; Chen, Y.; Yang, Z.; Ding, G. Inhibition of Prostate Cancer DU-145 Cells Proliferation by Anthopleura Anjunae Oligopeptide (YVPGP) via PI3K/AKT/mTOR Signaling Pathway. Mar. Drugs 2018, 16, 325. [Google Scholar] [CrossRef] [PubMed]
  46. Feng, C.; Zhang, Y.; Yang, M.; Huang, B.; Zhou, Y. Collagen-Derived N-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype. Stem Cells 2015, 33, 3558–3568. [Google Scholar] [CrossRef] [PubMed]
  47. Kwon, Y.W.; Heo, S.C.; Lee, T.W.; Park, G.T.; Yoon, J.W.; Jang, I.H.; Kim, S.-C.; Ko, H.-C.; Ryu, Y.; Kang, H.; et al. N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells. Sci. Rep. 2017, 7, 43057. [Google Scholar] [CrossRef]
  48. Thaler, R.; Zwerina, J.; Rumpler, M.; Spitzer, S.; Gamsjaeger, S.; Paschalis, E.P.; Klaushofer, K.; Varga, F. Homocysteine Induces Serum Amyloid A3 in Osteoblasts via Unlocking RGD-Motifs in Collagen. FASEB J. 2013, 27, 446–463. [Google Scholar] [CrossRef] [PubMed]
  49. Taubenberger, A.V.; Woodruff, M.A.; Bai, H.; Muller, D.J.; Hutmacher, D.W. The Effect of Unlocking RGD-Motifs in Collagen I on Pre-Osteoblast Adhesion and Differentiation. Biomaterials 2010, 31, 2827–2835. [Google Scholar] [CrossRef]
  50. Davis, G.E. Affinity of Integrins for Damaged Extracellular Matrix: Avβ3 Binds to Denatured Collagen Type I through RGD Sites. Biochem. Biophys. Res. Commun. 1992, 182, 1025–1031. [Google Scholar] [CrossRef]
  51. Davis, G.E.; Bayless, K.J.; Davis, M.J.; Meininger, G.A. Regulation of Tissue Injury Responses by the Exposure of Matricryptic Sites within Extracellular Matrix Molecules. Am. J. Pathol. 2000, 156, 1489–1498. [Google Scholar] [CrossRef] [PubMed]
  52. Ma, Z.; Mao, C.; Jia, Y.; Fu, Y.; Kong, W. Extracellular Matrix Dynamics in Vascular Remodeling. Am. J. Physiol.—Cell Physiol. 2020, 319, C481–C499. [Google Scholar] [CrossRef] [PubMed]
  53. Argilés, Á.; Siwy, J.; Duranton, F.; Gayrard, N.; Dakna, M.; Lundin, U.; Osaba, L.; Delles, C.; Mourad, G.; Weinberger, K.M.; et al. CKD273, a New Proteomics Classifier Assessing CKD and Its Prognosis. PLoS ONE 2013, 8, e62837. [Google Scholar] [CrossRef]
  54. Hobson, S.; Mavrogeorgis, E.; He, T.; Siwy, J.; Ebert, T.; Kublickiene, K.; Stenvinkel, P.; Mischak, H. Urine Peptidome Analysis Identifies Common and Stage-Specific Markers in Early Versus Advanced CKD. Proteomes 2023, 11, 25. [Google Scholar] [CrossRef] [PubMed]
  55. Campbell, R.T.; Jasilek, A.; Mischak, H.; Nkuipou-Kenfack, E.; Latosinska, A.; Welsh, P.I.; Jackson, C.E.; Cannon, J.; McConnachie, A.; Delles, C.; et al. The Novel Urinary Proteomic Classifier HF1 Has Similar Diagnostic and Prognostic Utility to BNP in Heart Failure. ESC Heart Fail. 2020, 7, 1595–1604. [Google Scholar] [CrossRef]
  56. Bannaga, A.S.; Metzger, J.; Kyrou, I.; Voigtländer, T.; Book, T.; Melgarejo, J.; Latosinska, A.; Pejchinovski, M.; Staessen, J.A.; Mischak, H.; et al. Discovery, Validation and Sequencing of Urinary Peptides for Diagnosis of Liver Fibrosis-A Multicentre Study. EBioMedicine 2020, 62, 103083. [Google Scholar] [CrossRef]
  57. Diaz-Ricart, M.; Torramade-Moix, S.; Pascual, G.; Palomo, M.; Moreno-Castaño, A.B.; Martinez-Sanchez, J.; Vera, M.; Cases, A.; Escolar, G. Endothelial Damage, Inflammation and Immunity in Chronic Kidney Disease. Toxins 2020, 12, 361. [Google Scholar] [CrossRef]
  58. Godo, S.; Shimokawa, H. Endothelial Functions. Arterioscler. Thromb. Vasc. Biol. 2017, 37, e108–e114. [Google Scholar] [CrossRef]
  59. Magalhães, P.; Pontillo, C.; Pejchinovski, M.; Siwy, J.; Krochmal, M.; Makridakis, M.; Carrick, E.; Klein, J.; Mullen, W.; Jankowski, J.; et al. Comparison of Urine and Plasma Peptidome Indicates Selectivity in Renal Peptide Handling. Proteom. Clin. Appl. 2018, 12, e1700163. [Google Scholar] [CrossRef]
  60. Ganapathy, V.; Leibach, F.H. Carrier-Mediated Reabsorption of Small Peptides in Renal Proximal Tubule. Am. J. Physiol.-Ren. Physiol. 1986, 251, F945–F953. [Google Scholar] [CrossRef]
  61. Fiedler, L.R.; Schönherr, E.; Waddington, R.; Niland, S.; Seidler, D.G.; Aeschlimann, D.; Eble, J.A. Decorin Regulates Endothelial Cell Motility on Collagen I through Activation of Insulin-like Growth Factor I Receptor and Modulation of Alpha2beta1 Integrin Activity. J. Biol. Chem. 2008, 283, 17406–17415. [Google Scholar] [CrossRef]
  62. Nakamura, J.; Shigematsu, S.; Yamauchi, K.; Takeda, T.; Yamazaki, M.; Kakizawa, T.; Hashizume, K. Biphasic Function of Focal Adhesion Kinase in Endothelial Tube Formation Induced by Fibril-Forming Collagens. Biochem. Biophys. Res. Commun. 2008, 374, 699–703. [Google Scholar] [CrossRef]
  63. Senger, D.R.; Perruzzi, C.A.; Streit, M.; Koteliansky, V.E.; de Fougerolles, A.R.; Detmar, M. The A1β1 and A2β1 Integrins Provide Critical Support for Vascular Endothelial Growth Factor Signaling, Endothelial Cell Migration, and Tumor Angiogenesis. Am. J. Pathol. 2002, 160, 195–204. [Google Scholar] [CrossRef]
  64. de Kruijf, P.; Lim, H.D.; Overbeek, S.A.; Zaman, G.J.R.; Kraneveld, A.D.; Folkerts, G.; Leurs, R.; Smit, M.J. The Collagen-Breakdown Product N-Acetyl-Proline-Glycine-Proline (N-α-PGP) Does Not Interact Directly with Human CXCR1 and CXCR2. Eur. J. Pharmacol. 2010, 643, 29–33. [Google Scholar] [CrossRef]
  65. Knight, C.G.; Morton, L.F.; Onley, D.J.; Peachey, A.R.; Messent, A.J.; Smethurst, P.A.; Tuckwell, D.S.; Farndale, R.W.; Barnes, M.J. Identification in Collagen Type I of an Integrin A2β1-Binding Site Containing an Essential GER Sequence. J. Biol. Chem. 1998, 273, 33287–33294. [Google Scholar] [CrossRef]
  66. Siwy, J.; Mullen, W.; Golovko, I.; Franke, J.; Zürbig, P. Human Urinary Peptide Database for Multiple Disease Biomarker Discovery. Proteom.—Clin. Appl. 2011, 5, 367–374. [Google Scholar] [CrossRef]
  67. COL1A1—Collagen Alpha-1(I) Chain—Homo Sapiens (Human)|UniProtKB|UniProt. Available online: https://www.uniprot.org/uniprotkb/P02452/entry (accessed on 9 January 2023).
  68. Abramson, J.; Adler, J.; Dunger, J.; Evans, R.; Green, T.; Pritzel, A.; Ronneberger, O.; Willmore, L.; Ballard, A.J.; Bambrick, J.; et al. Accurate Structure Prediction of Biomolecular Interactions with AlphaFold 3. Nature 2024, 630, 493–500. [Google Scholar] [CrossRef] [PubMed]
  69. ITGA2—Integrin Alpha-2—Homo Sapiens (Human)|UniProtKB|UniProt. Available online: https://www.uniprot.org/uniprotkb/P17301/entry (accessed on 19 September 2024).
  70. ITGB1—Integrin Beta-1—Homo Sapiens (Human)|UniProtKB|UniProt. Available online: https://www.uniprot.org/uniprotkb/P05556/entry (accessed on 19 September 2024).
  71. Harmalkar, A.; Lyskov, S.; Gray, J.J. Reliable Protein—Protein Docking with AlphaFold, Rosetta, and Replica Exchange. eLife 2025, 13, RP94029. [Google Scholar] [CrossRef]
  72. Burke, D.F.; Bryant, P.; Barrio-Hernandez, I.; Memon, D.; Pozzati, G.; Shenoy, A.; Zhu, W.; Dunham, A.S.; Albanese, P.; Keller, A.; et al. Towards a Structurally Resolved Human Protein Interaction Network. Nat. Struct. Mol. Biol. 2023, 30, 216–225. [Google Scholar] [CrossRef]
  73. Chiang, Y.; Hui, W.-H.; Chang, S.-W. Encoding Protein Dynamic Information in Graph Representation for Functional Residue Identification. Cell Rep. Phys. Sci. 2022, 3, 100975. [Google Scholar] [CrossRef]
  74. Jiang, Y.; Xu, C.; Zhao, Y.; Ji, Y.; Wang, X.; Liu, Y. LINC00926 Is Involved in Hypoxia-Induced Vascular Endothelial Cell Dysfunction via miR-3194-5p Regulating JAK1/STAT3 Signaling Pathway. Eur. J. Histochem. 2023, 67. [Google Scholar] [CrossRef]
  75. He, Q.; He, W.; Dong, H.; Guo, Y.; Yuan, G.; Shi, X.; Wang, D.; Lu, F. Role of Liver Sinusoidal Endothelial Cell in Metabolic Dysfunction-Associated Fatty Liver Disease. Cell Commun. Signal. CCS 2024, 22, 346. [Google Scholar] [CrossRef]
  76. Sgarioto, M.; Vigneron, P.; Patterson, J.; Malherbe, F.; Nagel, M.-D.; Egles, C. Collagen Type I Together with Fibronectin Provide a Better Support for Endothelialization. Comptes Rendus Biol. 2012, 335, 520–528. [Google Scholar] [CrossRef] [PubMed]
  77. Lindsey, M.L.; Iyer, R.P.; Zamilpa, R.; Yabluchanskiy, A.; DeLeon-Pennell, K.Y.; Hall, M.E.; Kaplan, A.; Zouein, F.A.; Bratton, D.; Flynn, E.R.; et al. A Novel Collagen Matricryptin Reduces Left Ventricular Dilation Post-Myocardial Infarction by Promoting Scar Formation and Angiogenesis. J. Am. Coll. Cardiol. 2015, 66, 1364–1374. [Google Scholar] [CrossRef]
  78. Murdoch, C.; Monk, P.N.; Finn, A. Cxc Chemokine Receptor Expression On Human Endothelial Cells. Cytokine 1999, 11, 704–712. [Google Scholar] [CrossRef]
  79. Emsley, J.; Knight, C.G.; Farndale, R.W.; Barnes, M.J. Structure of the Integrin A2β1-Binding Collagen Peptide. J. Mol. Biol. 2004, 335, 1019–1028. [Google Scholar] [CrossRef]
  80. Emsley, J.; Knight, C.G.; Farndale, R.W.; Barnes, M.J.; Liddington, R.C. Structural Basis of Collagen Recognition by Integrin A2β1. Cell 2000, 101, 47–56. [Google Scholar] [CrossRef] [PubMed]
  81. Kamata, T.; Liddington, R.C.; Takada, Y. Interaction between Collagen and the A2 I-Domain of Integrin A2β1: Critical Role Of Conserved Residues In The Metal Ion-Dependent Adhesion Site (Midas) Region. J. Biol. Chem. 1999, 274, 32108–32111. [Google Scholar] [CrossRef] [PubMed]
  82. Smith, C.; Estavillo, D.; Emsley, J.; Bankston, L.A.; Liddington, R.C.; Cruz, M.A. Mapping the Collagen-Binding Site in the I Domain of the Glycoprotein Ia/IIa (Integrin A2β1). J. Biol. Chem. 2000, 275, 4205–4209. [Google Scholar] [CrossRef]
  83. Potel, C.M.; Lemeer, S.; Heck, A.J.R. Phosphopeptide Fragmentation and Site Localization by Mass Spectrometry: An Update. Anal. Chem. 2019, 91, 126–141. [Google Scholar] [CrossRef]
  84. Martin, D.M.A.; Nett, I.R.E.; Vandermoere, F.; Barber, J.D.; Morrice, N.A.; Ferguson, M.A.J. Prophossi: Automating Expert Validation of Phosphopeptide-Spectrum Matches from Tandem Mass Spectrometry. Bioinformatics 2010, 26, 2153–2159. [Google Scholar] [CrossRef]
  85. Ostrowska-Podhorodecka, Z.; Ding, I.; Lee, W.; Tanic, J.; Abbasi, S.; Arora, P.D.; Liu, R.S.; Patteson, A.E.; Janmey, P.A.; McCulloch, C.A. Vimentin Tunes Cell Migration on Collagen by Controlling Β1 Integrin Activation and Clustering. J. Cell Sci. 2021, 134, jcs254359. [Google Scholar] [CrossRef] [PubMed]
  86. Vidyasagar, A.; Wilson, N.A.; Djamali, A. Heat Shock Protein 27 (HSP27): Biomarker of Disease and Therapeutic Target. Fibrogenes. Tissue Repair 2012, 5, 7. [Google Scholar] [CrossRef]
  87. Kostenko, S.; Moens, U. Heat Shock Protein 27 Phosphorylation: Kinases, Phosphatases, Functions and Pathology. Cell. Mol. Life Sci. 2009, 66, 3289–3307. [Google Scholar] [CrossRef]
  88. Evans, I.M.; Britton, G.; Zachary, I.C. Vascular Endothelial Growth Factor Induces Heat Shock Protein (HSP) 27 Serine 82 Phosphorylation and Endothelial Tubulogenesis via Protein Kinase D and Independent of P38 Kinase. Cell. Signal. 2008, 20, 1375–1384. [Google Scholar] [CrossRef]
  89. Shah, K.; Shokat, K.M. A Chemical Genetic Screen for Direct V-Src Substrates Reveals Ordered Assembly of a Retrograde Signaling Pathway. Chem. Biol. 2002, 9, 35–47. [Google Scholar] [CrossRef]
  90. Li, Y.; Sun, S.; Zhang, H.; Jing, Y.; Ji, X.; Wan, Q.; Liu, Y. CALU Promotes Lung Adenocarcinoma Progression by Enhancing Cell Proliferation, Migration and Invasion. Respir. Res. 2024, 25, 267. [Google Scholar] [CrossRef]
  91. Chevet, E.; Wong, H.N.; Gerber, D.; Cochet, C.; Fazel, A.; Cameron, P.H.; Gushue, J.N.; Thomas, D.Y.; Bergeron, J.J.M. Phosphorylation by CK2 and MAPK Enhances Calnexin Association with Ribosomes. EMBO J. 1999, 18, 3655–3666. [Google Scholar] [CrossRef] [PubMed]
  92. Augustin-Voss, H.G.; Pauli, B.U. Migrating Endothelial Cells Are Distinctly Hyperglycosylated and Express Specific Migration-Associated Cell Surface Glycoproteins. J. Cell Biol. 1992, 119, 483–491. [Google Scholar] [CrossRef] [PubMed]
  93. Worth, D.C.; Daly, C.N.; Geraldo, S.; Oozeer, F.; Gordon-Weeks, P.R. Drebrin Contains a Cryptic F-Actin–Bundling Activity Regulated by Cdk5 Phosphorylation. J. Cell Biol. 2013, 202, 793–806. [Google Scholar] [CrossRef]
  94. Connors, W.L.; Jokinen, J.; White, D.J.; Puranen, J.S.; Kankaanpaöaö, P.; Upla, P.; Tulla, M.; Johnson, M.S.; Heino, J. Two Synergistic Activation Mechanisms of A2β1 Integrin-Mediated Collagen Binding. J. Biol. Chem. 2007, 282, 14675–14683. [Google Scholar] [CrossRef]
  95. Medina-Leyte, D.J.; Domínguez-Pérez, M.; Mercado, I.; Villarreal-Molina, M.T.; Jacobo-Albavera, L. Use of Human Umbilical Vein Endothelial Cells (HUVEC) as a Model to Study Cardiovascular Disease: A Review. Appl. Sci. 2020, 10, 938. [Google Scholar] [CrossRef]
  96. Mischak, H.; Kolch, W.; Aivaliotis, M.; Bouyssié, D.; Court, M.; Dihazi, H.; Dihazi, G.H.; Franke, J.; Garin, J.; de Peredo, A.G.; et al. Comprehensive Human Urine Standards for Comparability and Standardization in Clinical Proteome Analysis. Proteom.—Clin. Appl. 2010, 4, 464–478. [Google Scholar] [CrossRef] [PubMed]
  97. Mokou, M.; Klein, J.; Makridakis, M.; Bitsika, V.; Bascands, J.-L.; Saulnier-Blache, J.S.; Mullen, W.; Sacherer, M.; Zoidakis, J.; Pieske, B.; et al. Proteomics Based Identification of KDM5 Histone Demethylases Associated with Cardiovascular Disease. EBioMedicine 2019, 41, 91–104. [Google Scholar] [CrossRef]
  98. Megarioti, A.H.; Primo, C.; Kapetanakis, G.C.; Athanasopoulos, A.; Sophianopoulou, V.; André, B.; Gournas, C. The Bul1/2 Alpha-Arrestins Promote Ubiquitylation and Endocytosis of the Can1 Permease upon Cycloheximide-Induced TORC1-Hyperactivation. Int. J. Mol. Sci. 2021, 22, 10208. [Google Scholar] [CrossRef]
  99. Megarioti, A.H.; Esch, B.M.; Athanasopoulos, A.; Koulouris, D.; Makridakis, M.; Lygirou, V.; Samiotaki, M.; Zoidakis, J.; Sophianopoulou, V.; André, B.; et al. Ferroptosis-Protective Membrane Domains in Quiescence. Cell Rep. 2023, 42, 113561. [Google Scholar] [CrossRef]
  100. Makridakis, M.; Vlahou, A. GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue. Methods Mol. Biol. 2018, 1788, 165–175. [Google Scholar] [CrossRef] [PubMed]
  101. Claeys, T.; Van Den Bossche, T.; Perez-Riverol, Y.; Gevaert, K.; Vizcaíno, J.A.; Martens, L. lesSDRF Is More: Maximizing the Value of Proteomics Data through Streamlined Metadata Annotation. Nat. Commun. 2023, 14, 6743. [Google Scholar] [CrossRef] [PubMed]
Ijms 26 07480 g001
Figure 1. Overview of the methodology and results. First, we investigated the effect of the peptides on cell migration (A). Next, we confirmed the presence of the suggested receptor (integrin α2β1) based on the literature and modeled the interaction of the biologically active Peptide 1 with the receptor using AlphaFold 3 (B). Lastly, we investigated the signaling pathways inhibited by the biologically active peptide (C).
Figure 1. Overview of the methodology and results. First, we investigated the effect of the peptides on cell migration (A). Next, we confirmed the presence of the suggested receptor (integrin α2β1) based on the literature and modeled the interaction of the biologically active Peptide 1 with the receptor using AlphaFold 3 (B). Lastly, we investigated the signaling pathways inhibited by the biologically active peptide (C).
Ijms 26 07480 g001
Ijms 26 07480 g002
Figure 2. (A) boxplots of the average number of migrated cells per biological replicate, per treatment condition. Results from three replicates are shown, except for the negative control (n = 11), Collagen type I (COL(I))-treated cells (n = 12), and peptide 1 + COL(I) (n = 5). Data analysis utilized a negative binomial generalized linear model, followed by post hoc Welch two-sample t-tests (p < 0.05). (B–D) Representative microscopic images of the migration experiment for COL(I) (B), negative control (C), and Peptide 1 + COL(I) (D). Images taken at 10× magnification.
Figure 2. (A) boxplots of the average number of migrated cells per biological replicate, per treatment condition. Results from three replicates are shown, except for the negative control (n = 11), Collagen type I (COL(I))-treated cells (n = 12), and peptide 1 + COL(I) (n = 5). Data analysis utilized a negative binomial generalized linear model, followed by post hoc Welch two-sample t-tests (p < 0.05). (B–D) Representative microscopic images of the migration experiment for COL(I) (B), negative control (C), and Peptide 1 + COL(I) (D). Images taken at 10× magnification.
Ijms 26 07480 g002
Ijms 26 07480 g003
Figure 3. (A) Flow cytometry analysis of the integrin α2β1 receptor. The plot on the left shows that the integrin α2 receptor is present on more than half of the cells, while the plot on the right shows a similar distribution for integrin β1. (B) The left panel shows the AlphaFold 3 predictions of the integrin receptor–Peptide 1 complex. The integrin α2 subunit is represented in orange, integrin β1 in purple, Peptide 1 in green, and the position of the cell membrane relative to the predicted peptide–receptor complex is indicated by the gray double disc (representing the lipid bilayer). Residues predicted to be within 8Å of each other are considered interacting, as defined by AlphaFold 3 and widely accepted in the field (see for example [68,71,72,73]), and are visualized in more detail with Mol* software (https://molstar.org/viewer/, accessed on 08 June 2025). The predicted integrin α2 receptor–Peptide 1 interaction is enlarged on the right (other residues were excluded from the right-handed image to increase clarity). Dashed lines indicate predicted interactions, with gray lines indicating weak and blue lines indicating strong interactions. 1: Peptide 1 residue 242G and 154N on integrin α2, 2: Peptide 1 residue 242G with 155S on integrin α2, 3: Peptide 1 residue 243E with integrin α2 residue 155S, 4: Peptide 1 residue 245G with integrin α2 residue 219D and 5: Peptide 1 residue 246P with integrin α2 residue 219D. Remaining bonds are between integrin α2 residues internally or peptide 1 internally. (C) Schematic representation of the residues relevant to the predicted peptide 1–integrin α2β1 interaction are shown. Their names are colored according to the molecule they belong to (orange for integrin α2 and green for peptide 1), dashed lines indicate predicted interactions, with gray lines indicating weak and blue lines indicating strong interactions. The numeration of the bonds is the same as for panel (B). Images in part (C) were drawn using PepDraw (https://pepdraw.com/, accessed on 1 August 2025).
Figure 3. (A) Flow cytometry analysis of the integrin α2β1 receptor. The plot on the left shows that the integrin α2 receptor is present on more than half of the cells, while the plot on the right shows a similar distribution for integrin β1. (B) The left panel shows the AlphaFold 3 predictions of the integrin receptor–Peptide 1 complex. The integrin α2 subunit is represented in orange, integrin β1 in purple, Peptide 1 in green, and the position of the cell membrane relative to the predicted peptide–receptor complex is indicated by the gray double disc (representing the lipid bilayer). Residues predicted to be within 8Å of each other are considered interacting, as defined by AlphaFold 3 and widely accepted in the field (see for example [68,71,72,73]), and are visualized in more detail with Mol* software (https://molstar.org/viewer/, accessed on 08 June 2025). The predicted integrin α2 receptor–Peptide 1 interaction is enlarged on the right (other residues were excluded from the right-handed image to increase clarity). Dashed lines indicate predicted interactions, with gray lines indicating weak and blue lines indicating strong interactions. 1: Peptide 1 residue 242G and 154N on integrin α2, 2: Peptide 1 residue 242G with 155S on integrin α2, 3: Peptide 1 residue 243E with integrin α2 residue 155S, 4: Peptide 1 residue 245G with integrin α2 residue 219D and 5: Peptide 1 residue 246P with integrin α2 residue 219D. Remaining bonds are between integrin α2 residues internally or peptide 1 internally. (C) Schematic representation of the residues relevant to the predicted peptide 1–integrin α2β1 interaction are shown. Their names are colored according to the molecule they belong to (orange for integrin α2 and green for peptide 1), dashed lines indicate predicted interactions, with gray lines indicating weak and blue lines indicating strong interactions. The numeration of the bonds is the same as for panel (B). Images in part (C) were drawn using PepDraw (https://pepdraw.com/, accessed on 1 August 2025).
Ijms 26 07480 g003
Ijms 26 07480 g004
Figure 4. (A) Average abundance per treatment, with the proteins of which peptides display significantly differential abundance (after Benjamini–Hochberg correction) between treatments highlighted in bold and green. Data from twelve replicates are shown. (B) Breakdown of the average abundance per time point for the significant phospho-peptides shown in panel (A). Asterisks denote significant differences in abundance at specific time points and are displayed in the column corresponding to their highest abundance. For each time point and per treatment, four replicates were evaluated.
Figure 4. (A) Average abundance per treatment, with the proteins of which peptides display significantly differential abundance (after Benjamini–Hochberg correction) between treatments highlighted in bold and green. Data from twelve replicates are shown. (B) Breakdown of the average abundance per time point for the significant phospho-peptides shown in panel (A). Asterisks denote significant differences in abundance at specific time points and are displayed in the column corresponding to their highest abundance. For each time point and per treatment, four replicates were evaluated.
Ijms 26 07480 g004
Ijms 26 07480 g005
Figure 5. (A) Overview of the proposed signaling pathways activated by COL(I). COL(I) binding to the integrin α2β1 receptor triggers the activation of Src kinase [5,6] (which was not detected in this study), leading to the downstream activation of calumenin (CALU) [90]. Heat shock protein beta 1 (HSPB1) and CALU are involved in the formation of filopodia and subsequent endothelial cell migration [86,88,90,93]. Phosphorylated calnexin (CANX) aids in the folding of N-linked glycoproteins, which later move to the cell membrane and affect cell adhesion and migration [91,92]. (B) Peptide 1 likely blocks the interaction of COL(I) with integrin α2β1, thereby preventing the activation of the signaling cascade outlined in panel A. Abbreviations: VIM: vimentin, CALU: calumenin, HSPB1: heat shock protein beta 1, DBN1: drebrin.
Figure 5. (A) Overview of the proposed signaling pathways activated by COL(I). COL(I) binding to the integrin α2β1 receptor triggers the activation of Src kinase [5,6] (which was not detected in this study), leading to the downstream activation of calumenin (CALU) [90]. Heat shock protein beta 1 (HSPB1) and CALU are involved in the formation of filopodia and subsequent endothelial cell migration [86,88,90,93]. Phosphorylated calnexin (CANX) aids in the folding of N-linked glycoproteins, which later move to the cell membrane and affect cell adhesion and migration [91,92]. (B) Peptide 1 likely blocks the interaction of COL(I) with integrin α2β1, thereby preventing the activation of the signaling cascade outlined in panel A. Abbreviations: VIM: vimentin, CALU: calumenin, HSPB1: heat shock protein beta 1, DBN1: drebrin.
Ijms 26 07480 g005
Table 1. Overview of the shortlisted peptides investigated in this study. The table presents their names, sequences (with underline indicating motifs known to interact with cell receptors and ‘O’ denoting hydroxyproline), as well as the start and stop positions on the collagen type I alpha 1 chain (COLα1 (I). Additionally, the relative abundance in plasma and urine, along with the fold change in plasma over urine, as calculated in Magalhães et al. [59] are shown, rounded to two decimals.
Table 1. Overview of the shortlisted peptides investigated in this study. The table presents their names, sequences (with underline indicating motifs known to interact with cell receptors and ‘O’ denoting hydroxyproline), as well as the start and stop positions on the collagen type I alpha 1 chain (COLα1 (I). Additionally, the relative abundance in plasma and urine, along with the fold change in plasma over urine, as calculated in Magalhães et al. [59] are shown, rounded to two decimals.
Peptide NumberPeptide SequenceStart AA Position-
Stop AA Position		Rel. Abundance PlasmaRel. Abundance UrineFold Change Plasma/Urine
1NGDDGEAGKP
GRPGERGPOGO		229-2490.167.720.02
2NSGEOGAOGS
KGDTGAKGEO
GPVG		432-4550.446.870.06
3AOGDRGEOGP
OGPA		798-8110.345.600.06
4ADGQPGAKGE
OGDAGAKGDA
GPPGO		819-8430.814.290.19
5ANGAOGNDGA
KGDAGAOGAO
GSQGAOG		699-7250.192.730.07
6SOGSPGPDGK
TGPOGP		543-5580.791.710.46
7ESGREGAOGA
EGSOGRDGSO
GAKGDRGETGP		1011-10410.241.700.14
8AGPOGEAGKO
GEQGVOGDLG
AOGP		649-6720.681.150.59
9VGPOGPOGPO
GPPGPPS		177-11930.031.410.02
Table 2. Overview of the detected phospho-peptides with statistically significant differences in their abundance between Peptide 1 + COL(I)-treated samples and COL(I)-treated samples. Phosphorylated residues in the peptide sequences are underlined (for CALU and VIM, the phospho-site could not be determined). Statistically significant differences in abundance were assessed using the Wilcoxon rank test, first for each treatment and then for each treatment and time point. Abbreviations: LFC: log fold change; AHNAK: Neuroblast differentiation-associated protein AHNAK; CALU: Calumenin; CANX: Calnexin; DBN1: drebrin; HSPB1: heat shock protein beta 1; VIM: vimentin; COL (I): collagen type I. Peptides showing significant changes in at least two out of three time points are shown in bold. Means and standard deviation for each analysis are provided in Supplementary File, tab “differential abundance testing”.
Table 2. Overview of the detected phospho-peptides with statistically significant differences in their abundance between Peptide 1 + COL(I)-treated samples and COL(I)-treated samples. Phosphorylated residues in the peptide sequences are underlined (for CALU and VIM, the phospho-site could not be determined). Statistically significant differences in abundance were assessed using the Wilcoxon rank test, first for each treatment and then for each treatment and time point. Abbreviations: LFC: log fold change; AHNAK: Neuroblast differentiation-associated protein AHNAK; CALU: Calumenin; CANX: Calnexin; DBN1: drebrin; HSPB1: heat shock protein beta 1; VIM: vimentin; COL (I): collagen type I. Peptides showing significant changes in at least two out of three time points are shown in bold. Means and standard deviation for each analysis are provided in Supplementary File, tab “differential abundance testing”.
PeptideProtein the Peptide Is Derived fromAbundance (Peptide 1 + COL(I) vs. COL(I)Abundance (Peptide 1 + COL(I) vs. COL(I)) per Time Point
LPSGSGAASPTGSAVDIRAHNAK (Q09666)LFC: −1.08
p-value: 0.04		N.S.
VHNDAQSFDYDHDAFLGAEEAKCALU (O43852)LFC: −4.47
p-value: 0.01		At 7 min: LFC: −4.43, p-value: 0.02
At 30 min: LFC:
−4.43, p-value: 0.02
At 60 min: LFC:
−4.55, p-value: 0.02
AEEDEILNRSPRCANX (P27824)LFC: −2.68
p-value: 0.01		At 30 min: LFC:
−2.34, p-value: 0.03
At 60 min: LFC:
−2.79, p-value: 0.03
LSSPVLHRDBN1 (Q16643)LFC: 1
p-value: 0.01		At 30 min: LFC: 2.01, p-value: 0.03
QLSSGVSEIRHSPB1 (P04792)LFC: −4.31
p-value: 0.01		At 7 min: LFC: −4.95, p-value: 0.03
At 30 min: LFC:
−4.68, p-value: 0.03
At 60 min: LFC: −3.3, p-value: 0.03
SLYASSPGGVYATRVIM (P08670)LFC: −2.46
p-value: 0.01		At 30 min: LFC:
−3.97, p-value: 0.03
At 60 min: LFC:
−1.36, p-value: 0.03
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Devos, H.; Mina, I.K.; Paradeisi, F.; Makridakis, M.; Tserga, A.; Mokou, M.; Zoidakis, J.; Mischak, H.; Vlahou, A.; Latosinska, A.; et al. A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations. Int. J. Mol. Sci. 2025, 26, 7480. https://doi.org/10.3390/ijms26157480

AMA Style

Devos H, Mina IK, Paradeisi F, Makridakis M, Tserga A, Mokou M, Zoidakis J, Mischak H, Vlahou A, Latosinska A, et al. A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations. International Journal of Molecular Sciences. 2025; 26(15):7480. https://doi.org/10.3390/ijms26157480

Chicago/Turabian Style

Devos, Hanne, Ioanna K. Mina, Foteini Paradeisi, Manousos Makridakis, Aggeliki Tserga, Marika Mokou, Jerome Zoidakis, Harald Mischak, Antonia Vlahou, Agnieszka Latosinska, and et al. 2025. "A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations" International Journal of Molecular Sciences 26, no. 15: 7480. https://doi.org/10.3390/ijms26157480

APA Style

Devos, H., Mina, I. K., Paradeisi, F., Makridakis, M., Tserga, A., Mokou, M., Zoidakis, J., Mischak, H., Vlahou, A., Latosinska, A., & Roubelakis, M. G. (2025). A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations. International Journal of Molecular Sciences, 26(15), 7480. https://doi.org/10.3390/ijms26157480

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop