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Volume 26
Issue 15
10.3390/ijms26157251
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish

by
Kayo Sugitani
1,*,
Yuya Omori
1,
Takumi Mokuya
1,
Serika Hosoi
1,
Haruto Kobayashi
1,
Koki Miyata
1,
Yuhei Araiso
1 and
Yoshiki Koriyama
2
1
Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Ishikawa, Japan
2
'Graduate School and Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3 Minamitamagaki, Suzuka 513-8670, Mie, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(15), 7251; https://doi.org/10.3390/ijms26157251 (registering DOI)
Submission received: 30 May 2025 / Revised: 22 July 2025 / Accepted: 23 July 2025 / Published: 27 July 2025
(This article belongs to the Special Issue Molecular Mechanisms of Neurodegeneration and Neuroprotection for Retinal and Optic Nerve Diseases)
Review Reports Versions Notes

Abstract

Myeloid-derived growth factor (MYDGF), named in reference to its secretion from myeloid cells in bone marrow, is a novel protein with anti-apoptotic and tissue-repairing properties. MYDGF is found in various human tissues affected by different diseases. To date, however, MYDGF expression has yet to be reported in the nervous system. Herein, we demonstrate for the first time that MYDGF mRNA levels increased in the zebrafish retina 1 h after optic nerve injury (ONI). MYDGF-producing cells were located in the photoreceptors and infiltrating leukocytic cells. We prepared the retina for MYDGF gene knockdown by performing intraocular injections using either MYDGF-specific morpholino or the CRISPR/Cas9 system. Under these MYDGF-knockdown retinal conditions, anti-apoptotic Bcl-2 mRNA was suppressed; in comparison, apoptotic caspase-3 and inflammatory TNFα mRNA were significantly upregulated in the zebrafish retina after ONI compared to the control. Furthermore, heat shock factor 1 (HSF1) was evidently suppressed under these conditions, leading to a significant number of apoptotic neurons. These findings indicate that MYDGF is a key molecule in the stimulation of neuronal regeneration in the central nervous system.
Keywords: MYDGF; HSF1; Bcl-2; caspase-3; TNFα; retina; optic nerve regeneration; zebrafish: cell survival; CRISPR/Cas9 system MYDGF; HSF1; Bcl-2; caspase-3; TNFα; retina; optic nerve regeneration; zebrafish: cell survival; CRISPR/Cas9 system

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MDPI and ACS Style

Sugitani, K.; Omori, Y.; Mokuya, T.; Hosoi, S.; Kobayashi, H.; Miyata, K.; Araiso, Y.; Koriyama, Y. The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish. Int. J. Mol. Sci. 2025, 26, 7251. https://doi.org/10.3390/ijms26157251

AMA Style

Sugitani K, Omori Y, Mokuya T, Hosoi S, Kobayashi H, Miyata K, Araiso Y, Koriyama Y. The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish. International Journal of Molecular Sciences. 2025; 26(15):7251. https://doi.org/10.3390/ijms26157251

Chicago/Turabian Style

Sugitani, Kayo, Yuya Omori, Takumi Mokuya, Serika Hosoi, Haruto Kobayashi, Koki Miyata, Yuhei Araiso, and Yoshiki Koriyama. 2025. "The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish" International Journal of Molecular Sciences 26, no. 15: 7251. https://doi.org/10.3390/ijms26157251

APA Style

Sugitani, K., Omori, Y., Mokuya, T., Hosoi, S., Kobayashi, H., Miyata, K., Araiso, Y., & Koriyama, Y. (2025). The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish. International Journal of Molecular Sciences, 26(15), 7251. https://doi.org/10.3390/ijms26157251

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