Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes

Ma, Yun; Li, Fang; Wang, Xuyang; Sun, Qingpeng; Wang, Ronghuan; Zhao, Jiuran

doi:10.3390/ijms26125484

Open AccessArticle

Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes

by

Yun Ma

^1,†

,

Fang Li

^1,2,†,

Xuyang Wang

^1,2,

Qingpeng Sun

^2,*,

Ronghuan Wang

^1,* and

Jiuran Zhao

¹

Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

²

College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100096, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2025, 26(12), 5484; https://doi.org/10.3390/ijms26125484

Submission received: 8 May 2025 / Revised: 3 June 2025 / Accepted: 4 June 2025 / Published: 7 June 2025

(This article belongs to the Special Issue Advanced Plant Molecular Responses to Abiotic Stresses)

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Abstract

Arabidopsis seedlings grown in Petri dishes sealed with PE plastic wrap, PP parafilm, or NF surgical tape showed differences in growth, with PE plastic wrap resulting in a smaller size and fresh weight, followed by PP parafilm, compared to unsealed or NF surgical tape-sealed dishes. To investigate the basis of these phenotypic changes, transcriptome sequencing was performed. The results indicated that seedlings in dishes sealed with PE plastic wrap and PP parafilm exhibited over 1000 differentially expressed genes (DEGs) at 7 days. By 14 days, the number of DEGs had increased to over 2000 for each sealed condition. GO analysis showed that DEGs were commonly enriched in biological processes associated with the response to hypoxia under PE plastic wrap and PP parafilm sealing at both 7 and 14 days, as well as under NF surgical tape at 14 days. While O₂ levels showed no significant differences between sealed and unsealed conditions, CO₂ concentrations were notably lower in plates sealed with PE plastic wrap and PP parafilm. Furthermore, specific genes related to reduced size and delayed growth under sealed conditions were identified. In summary, sealing films negatively affect seedling growth, leading to significant shifts in gene expression profiles.

Keywords: sealingfilms; Arabidopsis seedlings; transcriptome; CO₂ concentrations

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MDPI and ACS Style

Ma, Y.; Li, F.; Wang, X.; Sun, Q.; Wang, R.; Zhao, J. Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes. Int. J. Mol. Sci. 2025, 26, 5484. https://doi.org/10.3390/ijms26125484

AMA Style

Ma Y, Li F, Wang X, Sun Q, Wang R, Zhao J. Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes. International Journal of Molecular Sciences. 2025; 26(12):5484. https://doi.org/10.3390/ijms26125484

Chicago/Turabian Style

Ma, Yun, Fang Li, Xuyang Wang, Qingpeng Sun, Ronghuan Wang, and Jiuran Zhao. 2025. "Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes" International Journal of Molecular Sciences 26, no. 12: 5484. https://doi.org/10.3390/ijms26125484

APA Style

Ma, Y., Li, F., Wang, X., Sun, Q., Wang, R., & Zhao, J. (2025). Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes. International Journal of Molecular Sciences, 26(12), 5484. https://doi.org/10.3390/ijms26125484

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Beware of Sealing Film of Petri Dishes!—Alters the Expression of a Large Number of Genes

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