Ethnicity-Based Variations in Focal Adhesion Kinase Signaling in Glioblastoma Gene Expression: A Study of the Puerto Rican Hispanic Population
Abstract
1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Tumor Specimens Acquisition
4.2. GBM Cells Purification from Tumor Specimens
4.3. Real-Time RT-PCR
4.4. Gene Expression Data
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Cantidio, F.S.; Gil, G.O.B.; Queiroz, I.N.; Regalin, M. Glioblastoma—Treatment and Obstacles. Rep. Pract. Oncol. Radiother. 2022, 27, 744–753. [Google Scholar] [CrossRef]
- Carrano, A.; Juarez, J.J.; Incontri, D.; Ibarra, A.; Cazares, H.G. Sex-Specific Differences in Glioblastoma. Cells 2021, 10, 1783. [Google Scholar] [CrossRef]
- Ostrom, Q.T.; Cote, D.J.; Ascha, M.; Kruchko, C.; Barnholtz-Sloan, J.S. Adult Glioma Incidence and Survival by Race or Ethnicity in the United States From 2000 to 2014. JAMA Oncol. 2018, 4, 1254–1262. [Google Scholar] [CrossRef] [PubMed]
- Walsh, K.M.; Neff, C.; Bondy, M.L.; Kruchko, C.; Huse, J.T.; Amos, C.I.; Barnholtz-Sloan, J.S.; Ostrom, Q.T. Influence of County-Level Geographic/Ancestral Origin on Glioma Incidence and Outcomes in US Hispanics. Neuro-Oncology 2023, 25, 398–406. [Google Scholar] [CrossRef] [PubMed]
- Ostrom, Q.T.; Cioffi, G.; Gittleman, H.; Patil, N.; Waite, K.; Kruchko, C.; Barnholtz-Sloan, J.S. CBTRUS Statistical Report: Primary Brain and Other Central Nervous System Tumors Diagnosed in the United States in 2012–2016. Neuro-Oncology 2019, 21 (Suppl. S5), v1–v100. [Google Scholar] [CrossRef] [PubMed]
- Rodriguez-Esteban, R.; Jiang, X. Differential Gene Expression in Disease: A Comparison between High-Throughput Studies and the Literature. BMC Med. Genom. 2017, 10, 59. [Google Scholar] [CrossRef] [PubMed]
- Nuñez, R.E.; Del Valle, M.M.; Ortiz, K.; Almodovar, L.; Kucheryavykh, L. Microglial Cytokines Induce Invasiveness and Proliferation of Human Glioblastoma through Pyk2 and FAK Activation. Cancers 2021, 13, 6160. [Google Scholar] [CrossRef] [PubMed]
- Riggs, D.; Yang, Z.; Kloss, J.; Loftus, J.C. The Pyk2 FERM Regulates Pyk2 Complex Formation and Phosphorylation. Cell Signal 2011, 23, 288–296. [Google Scholar] [CrossRef] [PubMed]
- Lipinski, C.A.; Tran, N.L.; Bay, C.; Kloss, J.; McDonough, W.S.; Beaudry, C.; Berens, M.E.; Loftus, J.C. Differential Role of Proline-Rich Tyrosine Kinase 2 and Focal Adhesion Kinase in Determining Glioblastoma Migration and Proliferation. Mol. Cancer Res. 2003, 1, 323–332. [Google Scholar]
- Riemenschneider, M.J.; Mueller, W.; Betensky, R.A.; Mohapatra, G.; Louis, D.N. In Situ Analysis of Integrin and Growth Factor Receptor Signaling Pathways in Human Glioblastomas Suggests Overlapping Relationships with Focal Adhesion Kinase Activation. Am. J. Pathol. 2005, 167, 1379–1387. [Google Scholar] [CrossRef]
- Xu, X.; Zhang, F.; Chen, X.; Ying, Q. MicroRNA-518b Functions as a Tumor Suppressor in Glioblastoma by Targeting PDGFRB. Mol. Med. Rep. 2017, 16, 5326–5332. [Google Scholar] [CrossRef] [PubMed]
- Wallmann, T.; Zhang, X.-M.; Wallerius, M.; Bolin, S.; Joly, A.-L.; Sobocki, C.; Leiss, L.; Jiang, Y.; Bergh, J.; Holland, E.C.; et al. Microglia Induce PDGFRB Expression in Glioma Cells to Enhance Their Migratory Capacity. IScience 2018, 9, 71–83. [Google Scholar] [CrossRef] [PubMed]
- Zhou, X.; Hao, Q.; Liao, P.; Luo, S.; Zhang, M.; Hu, G.; Liu, H.; Zhang, Y.; Cao, B.; Baddoo, M.; et al. Nerve Growth Factor Receptor Negates the Tumor Suppressor P53 as a Feedback Regulator. Elife 2016, 5, e15099. [Google Scholar] [CrossRef] [PubMed]
- Uribe, M.L.; Marrocco, I.; Yarden, Y. EGFR in Cancer: Signaling Mechanisms, Drugs, and Acquired Resistance. Cancers 2021, 13, 2748. [Google Scholar] [CrossRef] [PubMed]
- Leonard, D.A.; Merhige, M.E.; Williams, B.A.; Greene, R.S. Elevated Expression of the Interleukin-8 Receptors CXCR1 and CXCR2 in Peripheral Blood Cells in Obstructive Coronary Artery Disease. Coron. Artery Dis. 2011, 22, 491–496. [Google Scholar] [CrossRef]
- Qi, Z.-Y.; Shao, C.; Zhang, X.; Hui, G.-Z.; Wang, Z. Exogenous and Endogenous Hormones in Relation to Glioma in Women: A Meta-Analysis of 11 Case-Control Studies. PLoS ONE 2013, 8, e68695. [Google Scholar] [CrossRef] [PubMed]
- Lan, Y.-L.; Wang, X.; Lou, J.-C.; Ma, B.-B.; Xing, J.-S.; Zou, S.; Zhang, B. Update on the Effect of Exogenous Hormone Use on Glioma Risk in Women: A Meta-Analysis of Case-Control and Cohort Studies. J. Neurooncol. 2018, 137, 357–365. [Google Scholar] [CrossRef] [PubMed]
- Karlsson, S.A.; Studer, E.; Kettunen, P.; Westberg, L. Neural Androgen Receptors Modulate Gene Expression and Social Recognition But Not Social Investigation. Front. Behav. Neurosci. 2016, 10, 41. [Google Scholar] [CrossRef] [PubMed]
- Lu, S.; Simon, N.G.; Wang, Y.; Hu, S. Neural Androgen Receptor Regulation: Effects of Androgen and Antiandrogen. J. Neurobiol. 1999, 41, 505–512. [Google Scholar] [CrossRef]
- Via, M.; Gignoux, C.R.; Roth, L.A.; Fejerman, L.; Galanter, J.; Choudhry, S.; Toro-Labrador, G.; Viera-Vera, J.; Oleksyk, T.K.; Beckman, K.; et al. History Shaped the Geographic Distribution of Genomic Admixture on the Island of Puerto Rico. PLoS ONE 2011, 6, e16513. [Google Scholar] [CrossRef]
- Choudhry, S.; Burchard, E.G.; Borrell, L.N.; Tang, H.; Gomez, I.; Naqvi, M.; Nazario, S.; Torres, A.; Casal, J.; Martinez-Cruzado, J.C.; et al. Ancestry–Environment Interactions and Asthma Risk among Puerto Ricans. Am. J. Respir. Crit. Care Med. 2006, 174, 1088–1093. [Google Scholar] [CrossRef] [PubMed]
- Tortolero-Luna, G.; Torres-Cintrón, C.R.; Alvarado-Ortiz, M.; Ortiz-Ortiz, K.J.; Zavala-Zegarra, D.E.; Mora-Piñero, E. Incidence of Thyroid Cancer in Puerto Rico and the US by Racial/Ethnic Group, 2011–2015. BMC Cancer 2019, 19, 637. [Google Scholar] [CrossRef] [PubMed]
- Witkop, C.J.; Nuñez Babcock, M.; Rao, G.H.; Gaudier, F.; Summers, C.G.; Shanahan, F.; Harmon, K.R.; Townsend, D.; Sedano, H.O.; King, R.A. Albinism and Hermansky-Pudlak Syndrome in Puerto Rico. Bol. Asoc. Medica Puerto Rico 1990, 82, 333–339. [Google Scholar] [PubMed]
- Gahr, S.; Stoehr, R.; Geissinger, E.; Ficker, J.H.; Brueckl, W.M.; Gschwendtner, A.; Gattenloehner, S.; Fuchs, F.S.; Schulz, C.; Rieker, R.J.; et al. EGFR Mutational Status in a Large Series of Caucasian European NSCLC Patients: Data from Daily Practice. Br. J. Cancer 2013, 109, 1821–1828. [Google Scholar] [CrossRef] [PubMed]
- Zheng, R.; Yin, Z.; Alhatem, A.; Lyle, D.; You, B.; Jiang, A.S.; Liu, D.; Jobbagy, Z.; Wang, Q.; Aisner, S.; et al. Epidemiologic Features of NSCLC Gene Alterations in Hispanic Patients from Puerto Rico. Cancers 2020, 12, 3492. [Google Scholar] [CrossRef]
- Pinheiro, P.S.; Sherman, R.L.; Trapido, E.J.; Fleming, L.E.; Huang, Y.; Gomez-Marin, O.; Lee, D. Cancer Incidence in First Generation U.S. Hispanics: Cubans, Mexicans, Puerto Ricans, and New Latinos. Cancer Epidemiol. Biomark. Prev. 2009, 18, 2162–2169. [Google Scholar] [CrossRef] [PubMed]
- Passmore, S.R.; Kisicki, A.; Gilmore-Bykovskyi, A.; Green-Harris, G.; Edwards, D.F. “There’s Not Much We Can Do…” Researcher-Level Barriers to the Inclusion of Underrepresented Participants in Translational Research. J. Clin. Transl. Sci. 2022, 6, e4. [Google Scholar] [CrossRef]
- Cerami, E.; Gao, J.; Dogrusoz, U.; Gross, B.E.; Sumer, S.O.; Aksoy, B.A.; Jacobsen, A.; Byrne, C.J.; Heuer, M.L.; Larsson, E.; et al. The cBio Cancer Genomics Portal: An Open Platform for Exploring Multidimensional Cancer Genomics Data. Cancer Discov. 2012, 2, 401–404. [Google Scholar] [CrossRef] [PubMed]
- Gao, J.; Aksoy, B.A.; Dogrusoz, U.; Dresdner, G.; Gross, B.; Sumer, S.O.; Sun, Y.; Jacobsen, A.; Sinha, R.; Larsson, E.; et al. Integrative Analysis of Complex Cancer Genomics and Clinical Profiles Using the cBioPortal. Sci. Signal 2013, 6, pl1. [Google Scholar] [CrossRef]
- Li, B.; Dewey, C.N. RSEM: Accurate Transcript Quantification from RNA-Seq Data with or without a Reference Genome. BMC Bioinform. 2011, 12, 323. [Google Scholar] [CrossRef]
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Porter, T.; del Valle, M.M.; Kucheryavykh, L. Ethnicity-Based Variations in Focal Adhesion Kinase Signaling in Glioblastoma Gene Expression: A Study of the Puerto Rican Hispanic Population. Int. J. Mol. Sci. 2024, 25, 4947. https://doi.org/10.3390/ijms25094947
Porter T, del Valle MM, Kucheryavykh L. Ethnicity-Based Variations in Focal Adhesion Kinase Signaling in Glioblastoma Gene Expression: A Study of the Puerto Rican Hispanic Population. International Journal of Molecular Sciences. 2024; 25(9):4947. https://doi.org/10.3390/ijms25094947
Chicago/Turabian StylePorter, Tyrel, Miguel Mayol del Valle, and Lilia Kucheryavykh. 2024. "Ethnicity-Based Variations in Focal Adhesion Kinase Signaling in Glioblastoma Gene Expression: A Study of the Puerto Rican Hispanic Population" International Journal of Molecular Sciences 25, no. 9: 4947. https://doi.org/10.3390/ijms25094947
APA StylePorter, T., del Valle, M. M., & Kucheryavykh, L. (2024). Ethnicity-Based Variations in Focal Adhesion Kinase Signaling in Glioblastoma Gene Expression: A Study of the Puerto Rican Hispanic Population. International Journal of Molecular Sciences, 25(9), 4947. https://doi.org/10.3390/ijms25094947