Influence of N-Acetylglucosamine and Melatonin Interaction in Modeling the Photosynthetic Component and Metabolomics of Cucumber under Salinity Stress
Abstract
:1. Introduction
2. Results
2.1. Evaluation of Morphological Attributes of Cucumber Plants
2.2. Extraction and Quantification of Abscisic Acid and Measurement of Leaf Water Content
2.3. Quantification of Amino Acids and MDA Content in Cucumber Shoots
2.4. Determination of Chlorophyll, Chlorophyll Fluorescence Ratio, and Relative Water Content
2.5. OJIP Curve and Test Parameters
2.6. Measurement of Photosynthetic Rate (Pn), Stomatal Conductance (Gs), Transpiration Rate (E), and Substomatal CO2 Concentration
2.7. Quantification of K+, Mg+, Na+, Ca++ and P Content in Cucumber Shoots
2.8. Analysis of Antioxidants and Their Related Activity
3. Discussion
4. Material and Methods
4.1. Plant Experiments
4.2. Measurement of Chlorophyll Fluorescence
4.3. Quantification of K/Mg/Na/Ca/P
4.4. Analysis of Antioxidant Activity and Enzymes
4.4.1. SOD and CAT Assay and Quantification of Total Flavonoids and Phenolic Content
4.4.2. Measurement of Radical Scavenging Activity (DPPH, and ABTS)
4.5. Extraction and Quantification of Abscisic Acid
4.6. Analysis of Amino Acids Profile in Cucumber Shoots
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SL (cm) | RL (cm) | SFW (g) | RFW (g) | SDW (g) | RDW (g) | SD (mm) | |
---|---|---|---|---|---|---|---|
No Stress (NS) | |||||||
NT | 23.9 ± 0.59 b | 22.9 ± 0.22 b | 13.16 ± 0.35 b | 5.20 ± 0.13 b | 1.68 ± 0.05 c | 0.40 ± 0.02 c | 7.12 ± 0.03 b |
Mel | 26.2 ± 0.30 a | 24.5 ± 0.44 ab | 16.33 ± 0.29 a | 5.88 ± 0.15 ab | 1.89 ± 0.03 b | 0.48 ± 0.01 b | 7.22 ± 0.10 b |
GlcNAc | 27.0 ± 0.54 a | 24.8 ± 0.29 ab | 16.82 ± 0.30 a | 5.91 ± 0.16 ab | 1.84 ± 0.04 b | 0.46 ± 0.02 b | 7.79 ± 0.07 a |
Mel × GlcNAc | 28.7 ± 0.20 a | 26.9 ± 0.33 a | 17.30 ± 0.17 a | 6.64 ± 0.20 a | 1.96 ± 0.02 a | 0.52 ± 0.02 a | 7.96 ± 0.03 a |
Salinity Stress (SS) | |||||||
NT | 18.9 ± 0.43 b | 18.0 ± 0.34 c | 9.40 ± 0.57 c | 4.15 ± 0.37 c | 1.21 ± 0.06 c | 0.32 ± 0.02 c | 6.31 ± 0.09 c |
Mel | 20.6 ± 0.70 b | 22.1 ± 0.41 b | 10.34 ± 0.37 b | 6.21 ± 0.40 a | 1.59 ± 0.07 b | 0.44 ± 0.02 b | 7.03 ± 0.06 b |
GlcNAc | 20.8 ± 0.55 b | 22.8 ± 0.47 b | 11.41 ± 0.47 b | 5.76 ± 0.37 b | 1.49 ± 0.06 b | 0.45 ± 0.02 b | 7.06 ± 0.05 b |
Mel × GlcNAc | 24.6 ± 0.44 a | 25.1 ± 0.38 a | 13.63 ± 0.26 a | 6.30 ± 0.29 a | 1.77 ± 0.02 a | 0.50 ± 0.03 a | 7.82 ± 0.14 a |
ABS/RC | Absorption Flux per RC |
---|---|
TRo/RC | Trapping flux per RC |
ETo/RC | Electron transport flux per RC |
Fv/Fo | Ratio of variable fluorescence and minimum fluorescence |
TRo/ABS | Maximum quantum yield of the apparatus primary photochemical |
ETo/TRo | Electron movement efficiency |
DIo/CS | Dissipated energy flux per CS (at t = 0) |
ETo/CS | Electron transport flux (further than QA−) per RC |
TRo/CSo | Trapping flux (leading to QA reduction) per Cso |
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Kang, S.-M.; Adhikari, A.; Kwon, E.-H.; Gam, H.-J.; Jeon, J.R.; Woo, J.-I.; Lee, I.-J. Influence of N-Acetylglucosamine and Melatonin Interaction in Modeling the Photosynthetic Component and Metabolomics of Cucumber under Salinity Stress. Int. J. Mol. Sci. 2024, 25, 2844. https://doi.org/10.3390/ijms25052844
Kang S-M, Adhikari A, Kwon E-H, Gam H-J, Jeon JR, Woo J-I, Lee I-J. Influence of N-Acetylglucosamine and Melatonin Interaction in Modeling the Photosynthetic Component and Metabolomics of Cucumber under Salinity Stress. International Journal of Molecular Sciences. 2024; 25(5):2844. https://doi.org/10.3390/ijms25052844
Chicago/Turabian StyleKang, Sang-Mo, Arjun Adhikari, Eun-Hae Kwon, Ho-Jun Gam, Jin Ryeol Jeon, Ji-In Woo, and In-Jung Lee. 2024. "Influence of N-Acetylglucosamine and Melatonin Interaction in Modeling the Photosynthetic Component and Metabolomics of Cucumber under Salinity Stress" International Journal of Molecular Sciences 25, no. 5: 2844. https://doi.org/10.3390/ijms25052844