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Article
Peer-Review Record

Keratin Expression in Podocytopathies, ANCA-Associated Vasculitis and IgA Nephropathy

Int. J. Mol. Sci. 2024, 25(3), 1805; https://doi.org/10.3390/ijms25031805
by Paraskevi Pavlakou 1, Harikleia Gakiopoulou 2, Sonja Djudjaj 3, Kostas Palamaris 2, Maria Stella Trivyza 1, Kostas Stylianou 4, Dimitrios S. Goumenos 1, Evangelos Papachristou 1 and Marios Papasotiriou 1,*
Reviewer 1: Anonymous
Reviewer 2:
Int. J. Mol. Sci. 2024, 25(3), 1805; https://doi.org/10.3390/ijms25031805
Submission received: 15 December 2023 / Revised: 25 January 2024 / Accepted: 29 January 2024 / Published: 2 February 2024
(This article belongs to the Special Issue New Insights into Kidney Diseases)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript performed an immunohistochemical analysis of a keratin panel in a cohort of kidney biopsies, derived from patients with heterogeneous glomerulopathies and attempted to correlate their expression patterns with different clinicopathological parameters. They reported that Keratins, including keratins 7, 8, 18, and 19, an abundant component of renal epithelial cells, have the potential to be featured as a biomarker for kidney function prognosis in patients with glomerular diseases. The reviewer has some concerns that should be addressed.

1. The specificity of antibodies for keratins 7, 8, 18, and 19 should be clarified.

2. Localization of keratins 7, 8, 18, and 19 in the kidney should be determined by co-labeling with specific markers.

 

3. How about the levels of keratins 7, 8, 18, and 19 in the urine?

Author Response

Thank for your remarks. You can see our reply in the attached file. 

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

This is a potentially interesting article, although it remains somewhat anecdotal.

1. The term "hyperplastic glomerulopathy" is somewhat strange.

2. It is unclear why the authors choose ANCA-associated vasculitis.

3. As the authors mentioned most biopsy samples showed much chronic lesions. Thus, the expression of keratins may be sequale of chronic inflammation. If so, the implication of this study adding to our current knowledegs not relevant.

4. It is unclear how many glomeruli examined in each study.

5. I do not think the results obtained leading to be featured as a biomarler for kidney function as the authors concluded.

Comments on the Quality of English Language

May be OK, bout could be improved.

Author Response

Thank you for your comments. Please see the attached file for our reply.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

I have no further comments.

Reviewer 2 Report

Comments and Suggestions for Authors

I understand the authors' responses. I have no further concerns.

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