Next Article in Journal
Genome-Wide Expression Profiling and Phenotypic Analysis of Downstream Targets Identify the Fox Transcription Factor Jumeau as a Master Regulator of Cardiac Progenitor Cell Division
Next Article in Special Issue
ADCY5 Gene Affects Seasonal Reproduction in Dairy Goats by Regulating Ovarian Granulosa Cells Steroid Hormone Synthesis
Previous Article in Journal
Establishment and Validation of an Efficient Agrobacterium Tumefaciens-Mediated Transient Transformation System for Salix Psammophila
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 25
Issue 23
10.3390/ijms252312935
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis

by
Zhong Xu
1,†,
Junjing Wu
1,†,
Yujie Li
1,
Jiawei Zhou
1,
Yu Zhang
1,
Mu Qiao
1,
Yue Feng
1,
Hua Sun
1,
Zipeng Li
1,
Lianghua Li
1,
Favour Oluwapelumi Oyelami
2,
Xianwen Peng
1,* and
Shuqi Mei
1,*
1
Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan 430064, China
2
The John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(23), 12935; https://doi.org/10.3390/ijms252312935
Submission received: 4 November 2024 / Revised: 25 November 2024 / Accepted: 27 November 2024 / Published: 1 December 2024
(This article belongs to the Special Issue Molecular Genetics and Breeding Mechanisms in Domestic Animals: 3rd Edition)
Browse Figures
Review Reports Versions Notes

Abstract

Porcine skeletal muscle development is closely linked to meat production efficiency and quality. The accumulation of porcine intramuscular fat is influenced by the hyperplasia and hypertrophy of adipocytes within the muscle. However, the cellular profiles corresponding to the two stages of muscle development remain undetermined. Single-nucleus RNA sequencing (snRNA-seq) can elucidate cell subsets in tissues, capture gene expression at the individual cell level, and provide innovative perspectives for studying muscle and intramuscular fat formation. In this study, a total of 78,302 nuclei and 9 clusters of cells, which included fibro/adipogenic progenitor (FAP), myonuclei, adipocytes, and other cell types, of Xidu black pigs, were identified on Day 1 and Day 180. The pattern of cell clustering varied between the two developmental stages. Notably, the percentage of adipocytes in the Day 180 group was higher than in the Day 1 group (0.51% vs. 0.15%). Pseudo-time sequence analysis indicated that FAPs could differentiate into adipocytes and myonuclei cells, respectively. The THRSP gene was identified as a biomarker for swine intramuscular fat cells, and its down-regulation resulted in significant reduction in lipid droplet formation in porcine preadipocytes. Our research provides new insights into the cellular characteristics of intramuscular fat formation, which may facilitate the development of novel strategies to enhance intramuscular fat deposition and improve pork quality.
Keywords: pig; snRNA-seq; intramuscular fat; cell cluster; adipogenesis pig; snRNA-seq; intramuscular fat; cell cluster; adipogenesis

Share and Cite

MDPI and ACS Style

Xu, Z.; Wu, J.; Li, Y.; Zhou, J.; Zhang, Y.; Qiao, M.; Feng, Y.; Sun, H.; Li, Z.; Li, L.; et al. A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. Int. J. Mol. Sci. 2024, 25, 12935. https://doi.org/10.3390/ijms252312935

AMA Style

Xu Z, Wu J, Li Y, Zhou J, Zhang Y, Qiao M, Feng Y, Sun H, Li Z, Li L, et al. A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. International Journal of Molecular Sciences. 2024; 25(23):12935. https://doi.org/10.3390/ijms252312935

Chicago/Turabian Style

Xu, Zhong, Junjing Wu, Yujie Li, Jiawei Zhou, Yu Zhang, Mu Qiao, Yue Feng, Hua Sun, Zipeng Li, Lianghua Li, and et al. 2024. "A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis" International Journal of Molecular Sciences 25, no. 23: 12935. https://doi.org/10.3390/ijms252312935

APA Style

Xu, Z., Wu, J., Li, Y., Zhou, J., Zhang, Y., Qiao, M., Feng, Y., Sun, H., Li, Z., Li, L., Oyelami, F. O., Peng, X., & Mei, S. (2024). A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. International Journal of Molecular Sciences, 25(23), 12935. https://doi.org/10.3390/ijms252312935

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop