Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception

Chen, Hui-Wen; Ma, Chung-Pei; Chin, En; Chen, Yi-Tung; Wang, Teh-Cheng; Kuo, Yu-Ping; Su, Chia-Hao; Huang, Po-Jung; Tan, Bertrand Chin-Ming

doi:10.3390/ijms25115985

Open AccessArticle

Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception

by

Hui-Wen Chen

^1,2,†,

Chung-Pei Ma

^1,†

,

En Chin

^1,2,

Yi-Tung Chen

^1,3,

Teh-Cheng Wang

²,

Yu-Ping Kuo

³,

Chia-Hao Su

^4,5

,

Po-Jung Huang

^1,2,6

and

Bertrand Chin-Ming Tan

^1,2,7,8,*

¹

Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan

²

Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan

³

Molecular Medicine Research Center, Chang Gung University, Taoyuan 333, Taiwan

⁴

Center for General Education, Chang Gung University, Taoyuan 333, Taiwan

⁵

Department of Radiation Oncology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan

⁶

Genomic Medicine Core Laboratory, Lin-Kou Medical Center, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

⁷

Division of Colon and Rectal Surgery, Lin-Kou Medical Center, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

⁸

Department of Neurosurgery, Lin-Kou Medical Center, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2024, 25(11), 5985; https://doi.org/10.3390/ijms25115985

Submission received: 29 March 2024 / Revised: 22 May 2024 / Accepted: 28 May 2024 / Published: 30 May 2024

(This article belongs to the Section Molecular Neurobiology)

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Abstract

A-to-I RNA editing, catalyzed by the ADAR protein family, significantly contributes to the diversity and adaptability of mammalian RNA signatures, aligning with developmental and physiological needs. Yet, the functions of many editing sites are still to be defined. The Unc80 gene stands out in this context due to its brain-specific expression and the evolutionary conservation of its codon-altering editing event. The precise biological functions of Unc80 and its editing, however, are still largely undefined. In this study, we first demonstrated that Unc80 editing occurs in an ADAR2-dependent manner and is exclusive to the brain. By employing the CRISPR/Cas9 system to generate Unc80 knock-in mouse models that replicate the natural editing variations, our findings revealed that mice with the “gain-of-editing” variant (Unc80^G/G) exhibit heightened basal neuronal activity in critical olfactory regions, compared to the “loss-of-editing” (Unc80^S/S) counterparts. Moreover, an increase in glutamate levels was observed in the olfactory bulbs of Unc80^G/G mice, indicating altered neurotransmitter dynamics. Behavioral analysis of odor detection revealed distinctive responses to novel odors—both Unc80 deficient (Unc80^+/⁻) and Unc80^S/S mice demonstrated prolonged exploration times and heightened dishabituation responses. Further elucidating the olfactory connection of Unc80 editing, transcriptomic analysis of the olfactory bulb identified significant alterations in gene expression that corroborate the behavioral and physiological findings. Collectively, our research advances the understanding of Unc80’s neurophysiological functions and the impact of its editing on the olfactory sensory system, shedding light on the intricate molecular underpinnings of olfactory perception and neuronal activity.

Keywords: RNA editing; Unc80; olfactory perception; neuronal activity

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MDPI and ACS Style

Chen, H.-W.; Ma, C.-P.; Chin, E.; Chen, Y.-T.; Wang, T.-C.; Kuo, Y.-P.; Su, C.-H.; Huang, P.-J.; Tan, B.C.-M. Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception. Int. J. Mol. Sci. 2024, 25, 5985. https://doi.org/10.3390/ijms25115985

AMA Style

Chen H-W, Ma C-P, Chin E, Chen Y-T, Wang T-C, Kuo Y-P, Su C-H, Huang P-J, Tan BC-M. Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception. International Journal of Molecular Sciences. 2024; 25(11):5985. https://doi.org/10.3390/ijms25115985

Chicago/Turabian Style

Chen, Hui-Wen, Chung-Pei Ma, En Chin, Yi-Tung Chen, Teh-Cheng Wang, Yu-Ping Kuo, Chia-Hao Su, Po-Jung Huang, and Bertrand Chin-Ming Tan. 2024. "Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception" International Journal of Molecular Sciences 25, no. 11: 5985. https://doi.org/10.3390/ijms25115985

APA Style

Chen, H.-W., Ma, C.-P., Chin, E., Chen, Y.-T., Wang, T.-C., Kuo, Y.-P., Su, C.-H., Huang, P.-J., & Tan, B. C.-M. (2024). Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception. International Journal of Molecular Sciences, 25(11), 5985. https://doi.org/10.3390/ijms25115985

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Imbalance in Unc80 RNA Editing Disrupts Dynamic Neuronal Activity and Olfactory Perception

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