Next Article in Journal
Synergistic Anti-Inflammatory Activity of Lipid-Free Apolipoprotein (apo) A-I and CIGB-258 in Acute-Phase Zebrafish via Stabilization of the apoA-I Structure to Enhance Anti-Glycation and Antioxidant Activities
Next Article in Special Issue
An Antibody of the Secreted Isoform of Disintegrin and Metalloprotease 9 (sADAM9) Inhibits Epithelial–Mesenchymal Transition and Migration of Prostate Cancer Cell Lines
Previous Article in Journal
The Presence of TGFβ3 in Human Ovarian Intrafollicular Fluid and Its Involvement in Thromboxane Generation in Follicular Granulosa Cells through a Canonical TGFβRI, Smad2/3 Signaling Pathway and COX-2 Induction
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 25
Issue 10
10.3390/ijms25105559
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells

by
Yi-Hsuan Lin
1,
Tzu-Min Chen
1,
Yu-Ling Tsai
2,
Wen-Chiuan Tsai
2,
Hisao-Hsien Wang
3,
Ying Chen
1,*,† and
Sheng-Tang Wu
4,*,†
1
Department of Biology and Anatomy, National Defense Medical Center, Taipei 11490, Taiwan
2
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan
3
Department of Urology, Cheng Hsin General Hospital, Taipei 11490, Taiwan
4
Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(10), 5559; https://doi.org/10.3390/ijms25105559
Submission received: 26 April 2024 / Revised: 10 May 2024 / Accepted: 14 May 2024 / Published: 20 May 2024
(This article belongs to the Special Issue Male Genitourinary Tumors: Molecular Mechanisms and Potential Therapeutic Targets)
Browse Figures
Versions Notes

Abstract

Bladder cancer (BC) is a malignant tumor of the urinary system with high mortality and recurrence rates. Proteasome subunit type 4 (PSMB4) is highly expressed and has been identified as having oncogenic properties in a variety of cancer types. This study aimed to explore the effect of PSMB4 knockdown on the survival, migration, and angiogenesis of human bladder cancer cells with different degrees of malignancy. We analyzed the effects of PSMB4 knockdown in bladder cancer cells and endothelial cells in the tumor microenvironment. PSMB4 was highly expressed in patients with low- and high-grade urothelial carcinoma. Inhibition of PSMB4 reduced protein expression of focal adhesion kinase (FAK) and myosin light chain (MLC), leading to reduced migration. Furthermore, the suppression of PSMB4 decreased the levels of vascular endothelial factor B (VEGF-B), resulting in lower angiogenic abilities in human bladder cancer cells. PSMB4 inhibition affected the migratory ability of HUVECs and reduced VEGFR2 expression, consequently downregulating angiogenesis. In the metastatic animal model, PSMB4 knockdown reduced the relative volumes of lung tumors. Our findings suggest the role of PSMB4 as a potential target for therapeutic strategies against human bladder cancer.
Keywords: bladder cancer; PSMB4; migration; angiogenesis; tube formation bladder cancer; PSMB4; migration; angiogenesis; tube formation

Share and Cite

MDPI and ACS Style

Lin, Y.-H.; Chen, T.-M.; Tsai, Y.-L.; Tsai, W.-C.; Wang, H.-H.; Chen, Y.; Wu, S.-T. The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells. Int. J. Mol. Sci. 2024, 25, 5559. https://doi.org/10.3390/ijms25105559

AMA Style

Lin Y-H, Chen T-M, Tsai Y-L, Tsai W-C, Wang H-H, Chen Y, Wu S-T. The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells. International Journal of Molecular Sciences. 2024; 25(10):5559. https://doi.org/10.3390/ijms25105559

Chicago/Turabian Style

Lin, Yi-Hsuan, Tzu-Min Chen, Yu-Ling Tsai, Wen-Chiuan Tsai, Hisao-Hsien Wang, Ying Chen, and Sheng-Tang Wu. 2024. "The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells" International Journal of Molecular Sciences 25, no. 10: 5559. https://doi.org/10.3390/ijms25105559

APA Style

Lin, Y.-H., Chen, T.-M., Tsai, Y.-L., Tsai, W.-C., Wang, H.-H., Chen, Y., & Wu, S.-T. (2024). The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells. International Journal of Molecular Sciences, 25(10), 5559. https://doi.org/10.3390/ijms25105559

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop