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Article

Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals

by
Francesca Oliviero
1,*,
Chiara Baggio
1,
Marta Favero
1,2,
Amelia Carmela Damasco
1,
Carlotta Boscaro
3,4,
Davide Tietto
5,
Mattia Albiero
4,6,
Andrea Doria
1 and
Roberta Ramonda
1,*
1
Rheumatology Unit, Department of Medicine—DIMED, University of Padova, 35128 Padova, Italy
2
Medicine Unit 1, Ca’ Foncello Hospital, 31100 Treviso, Italy
3
Department of Medicine, University of Padova, 35128 Padova, Italy
4
Experimental Diabetology Lab, Veneto Institute of Molecular Medicine, 35129 Padova, Italy
5
Fisiored Clinic, 35136 Padova, Italy
6
Department of Surgery, Oncology and Gastroenterology, University of Padova, 35128 Padova, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(1), 393; https://doi.org/10.3390/ijms25010393
Submission received: 16 November 2023 / Revised: 22 December 2023 / Accepted: 26 December 2023 / Published: 27 December 2023
(This article belongs to the Special Issue Osteoarthritis: From Pathogenesis to Treatment)
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Abstract

The role of calcium pyrophosphate (CPP) crystals in osteoarthritis (OA) is still a matter of debate. With this study we aimed to investigate the inflammatory features of synovial fluid (SF) collected from patients with OA with CPP crystals compared with those without crystals. We also explored the effect of OA SF on monocytes response. SFs were collected from adult patients with OA and subdivided according to the presence of crystals. Local cellular and humoral inflammatory mediators were analysed in the SF samples. The expression levels of IL-1β, IL-18, CASP-1, NLRP3, and GAPDH were measured by RT-PCR in the cells obtained by pelleting the SF samples. For the in vitro study, a monocytic cell line was treated with selected SF samples. SF with CPP crystals showed a significant increase in inflammatory cellular indices and higher levels of IL-1β, IL-8, and caspase-1 transcript with respect to SF without crystals. Higher concentrations of VEGF were also observed in the early stages of the whole OA patients. THP-1 cells stimulated with OA SF released a significant amount of IL-1 β in culture supernatants. This study demonstrated that SF collected from patients with OA shows different inflammatory features depending on the presence of CPP crystals.
Keywords: osteoarthritis; synovial fluid; calcium pyrophosphate crystals; inflammation; interleukin-1 osteoarthritis; synovial fluid; calcium pyrophosphate crystals; inflammation; interleukin-1

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MDPI and ACS Style

Oliviero, F.; Baggio, C.; Favero, M.; Damasco, A.C.; Boscaro, C.; Tietto, D.; Albiero, M.; Doria, A.; Ramonda, R. Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals. Int. J. Mol. Sci. 2024, 25, 393. https://doi.org/10.3390/ijms25010393

AMA Style

Oliviero F, Baggio C, Favero M, Damasco AC, Boscaro C, Tietto D, Albiero M, Doria A, Ramonda R. Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals. International Journal of Molecular Sciences. 2024; 25(1):393. https://doi.org/10.3390/ijms25010393

Chicago/Turabian Style

Oliviero, Francesca, Chiara Baggio, Marta Favero, Amelia Carmela Damasco, Carlotta Boscaro, Davide Tietto, Mattia Albiero, Andrea Doria, and Roberta Ramonda. 2024. "Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals" International Journal of Molecular Sciences 25, no. 1: 393. https://doi.org/10.3390/ijms25010393

APA Style

Oliviero, F., Baggio, C., Favero, M., Damasco, A. C., Boscaro, C., Tietto, D., Albiero, M., Doria, A., & Ramonda, R. (2024). Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals. International Journal of Molecular Sciences, 25(1), 393. https://doi.org/10.3390/ijms25010393

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