Anti-Inflammatory Klotho Protein Serum Concentration Correlates with Interferon Gamma Expression Related to the Cellular Activity of Both NKT-like and T Cells in the Process of Human Aging
Abstract
:1. Introduction
2. Results
2.1. Content of Sulfhydryl (-SH) Groups in Serum Samples
2.2. α-Klotho Concentration in Serum Samples
2.3. Expression of TNF-α, IL-6 and IFN-γ in Non-Cultured CD3+ and CD3+CD56+ Cells
2.4. Expression of TNF-α in Cultured, Unstimulated or Stimulated with IL-2, LPS and PMA with Ionomycin CD3+ and CD3+CD56+ Cells
2.5. Expression of IL-6 in Cultured, Unstimulated or Stimulated with IL-2, LPS and PMA with Ionomycin CD3+ and CD3+CD56+ Cells
2.6. Expression of IFN-γ in Cultured, Non-Stimulated or Stimulated with IL-2, LPS and PMA with Ionomycin CD3+ and CD3+CD56+ Cells
2.7. Relationships between Age and Serum Concentrations of α-Klotho and -SH Groups
2.8. Relationships between the Studied Parameters Analyzed in Non-Cultured CD3+ and CD3+CD56+ Cells
2.9. Relationships between the Studied Parameters Analyzed in Cultivated CD3+ and CD3+CD56+ Cells
3. Discussion
4. Materials and Methods
4.1. Participants
4.2. Staining of Surface and Intracellular Antigens in Whole Blood Samples for Flow Cytometry
4.3. Preparation of Peripheral Blood Mononuclear Cell Cultures
4.4. Staining of Surface and Intracellular Antigens in Peripheral Blood Mononuclear Cells for Flow Cytometry
4.5. Serum Sample Collection
4.6. Determination of the Total –SH Group Content in Serum
4.7. Determination of α-Klotho Concentration in Serum
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell Population | CD3+ [%] | CD3+CD56+ [%] | ||||
---|---|---|---|---|---|---|
Parameter | TNF-α “0” | IL-6 “0” | IFN-γ “0” | TNF-α “0” | IL-6 “0” | IFN-γ “0” |
α-Klotho | ns | ns | 0.37 ** | ns | ns | 0.34 ** |
SH groups | 0.29 * | 0.38 ** | 0.4 ** | 0.41 ** | 0.45 *** | 0.36 ** |
Age | ns | −0.32 * | −0.52 *** | ns | −0.31* | −0.46 *** |
Cell Population | CD3+ [%] | CD3+CD56+ [%] | |||||
---|---|---|---|---|---|---|---|
Parameter | Stimulation Type | TNF-α | IL-6 | IFN-γ | TNF-α | IL-6 | IFN-γ |
α-Klotho | none | ns | ns | 0.34 ** | ns | ns | 0.25 * |
IL-2 | ns | ns | 0.44 *** | ns | ns | 0.35 ** | |
LPS | ns | ns | 0.49 *** | ns | ns | 0.39 *** | |
PMA+ion | ns | ns | 0.22 * | ns | ns | 0.32 ** | |
-SH groups | none | ns | ns | 0.3 ** | ns | ns | ns |
IL-2 | ns | ns | 0.36 ** | ns | ns | 0.22 * | |
LPS | ns | ns | 0.34 ** | ns | ns | ns | |
PMA+ion | ns | ns | ns | ns | ns | ns | |
Age | none | ns | ns | −0.32 ** | ns | ns | −0.24 * |
IL-2 | 0.23 * | ns | −0.37 *** | ns | ns | −0.26 * | |
LPS | 0.26 ** | ns | −0.4 *** | ns | ns | −0.23 * | |
PMA+ion | ns | ns | −0.33 *** | ns | ns | −0.42 *** |
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Kaszubowska, L.; Foerster, J.; Kaczor, J.J.; Karnia, M.J.; Kmieć, Z. Anti-Inflammatory Klotho Protein Serum Concentration Correlates with Interferon Gamma Expression Related to the Cellular Activity of Both NKT-like and T Cells in the Process of Human Aging. Int. J. Mol. Sci. 2023, 24, 8393. https://doi.org/10.3390/ijms24098393
Kaszubowska L, Foerster J, Kaczor JJ, Karnia MJ, Kmieć Z. Anti-Inflammatory Klotho Protein Serum Concentration Correlates with Interferon Gamma Expression Related to the Cellular Activity of Both NKT-like and T Cells in the Process of Human Aging. International Journal of Molecular Sciences. 2023; 24(9):8393. https://doi.org/10.3390/ijms24098393
Chicago/Turabian StyleKaszubowska, Lucyna, Jerzy Foerster, Jan Jacek Kaczor, Mateusz Jakub Karnia, and Zbigniew Kmieć. 2023. "Anti-Inflammatory Klotho Protein Serum Concentration Correlates with Interferon Gamma Expression Related to the Cellular Activity of Both NKT-like and T Cells in the Process of Human Aging" International Journal of Molecular Sciences 24, no. 9: 8393. https://doi.org/10.3390/ijms24098393