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Article

Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body

by
Anahí Armas-Tizapantzi
1,
José Luis Martínez y Pérez
2,
Francisco José Fernández
3,
Gerardo Mata
4,
Laura V. Hernández-Cuevas
2,
Elvia Ortiz Ortiz
5,
Edelmira García Nieto
2,
Araceli Tomasini
3,
Edgar Sierra-Palacios
6,
Jaime Marcial-Quino
2,* and
Alba Mónica Montiel-González
2,*
1
Doctorado en Ciencias Biológicas, Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala 90062, Mexico
2
Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Tlaxcala 90120, Mexico
3
Departamento de Biotecnología, CBS, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de Mexico 09340, Mexico
4
Instituto de Ecología, A.C., Xalapa 91073, Mexico
5
Facultad de Odontología, Universidad Autónoma de Tlaxcala, Tlaxcala 90000, Mexico
6
Colegio de Ciencias y Humanidades, Plantel Casa Libertad, Universidad Autónoma de la Ciudad de México, Ciudad de Mexico 09620, Mexico
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(9), 8143; https://doi.org/10.3390/ijms24098143
Submission received: 29 March 2023 / Revised: 26 April 2023 / Accepted: 26 April 2023 / Published: 2 May 2023
(This article belongs to the Special Issue Microbial Enzymes and Metabolites)
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Abstract

A wide variety of biological functions, including those involved in the morphogenesis process of basidiomycete fungi, have been attributed to laccase enzymes. In this work, RNA interference (RNAi) was used to evaluate the role of the laccase (lacc2) gene of Pleurotus ostreatus PoB. Previously, transformant strains of P. ostreatus were obtained and according to their level of silencing they were classified as light (T7), medium (T21) or severe (T26 and T27). The attenuation of the lacc2 gene in these transformants was determined by RT-PCR. Silencing of lacc2 resulted in a decrease in laccase activity between 30 and 55%, which depended on the level of laccase expression achieved. The silenced strains (T21, T26, and T27) displayed a delay in the development of mycelium on potato dextrose agar (PDA) medium, whereas in the cultures grown on wheat straw, we found that these strains were incapable of producing aerial mycelium, primordia, and fruiting bodies. Scanning electron microscopy (SEM) showed the presence of toxocyst-like structures. The highest abundance of these structures was observed in the wild-type (PoB) and T7 strains. However, the abundance of toxocysts decreased in the T21 and T26 strains, and in T27 they were not detected. These results suggest that the presence and abundance of toxocyst-like structures are directly related to the development of fruiting bodies. Furthermore, our data confirm that lacc2 is involved in the morphogenesis process of P. ostreatus.
Keywords: gene expression; transformants; morphogenesis; RNAi; scanning electron microscopy; solid culture gene expression; transformants; morphogenesis; RNAi; scanning electron microscopy; solid culture

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MDPI and ACS Style

Armas-Tizapantzi, A.; Martínez y Pérez, J.L.; Fernández, F.J.; Mata, G.; Hernández-Cuevas, L.V.; Ortiz Ortiz, E.; García Nieto, E.; Tomasini, A.; Sierra-Palacios, E.; Marcial-Quino, J.; et al. Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body. Int. J. Mol. Sci. 2023, 24, 8143. https://doi.org/10.3390/ijms24098143

AMA Style

Armas-Tizapantzi A, Martínez y Pérez JL, Fernández FJ, Mata G, Hernández-Cuevas LV, Ortiz Ortiz E, García Nieto E, Tomasini A, Sierra-Palacios E, Marcial-Quino J, et al. Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body. International Journal of Molecular Sciences. 2023; 24(9):8143. https://doi.org/10.3390/ijms24098143

Chicago/Turabian Style

Armas-Tizapantzi, Anahí, José Luis Martínez y Pérez, Francisco José Fernández, Gerardo Mata, Laura V. Hernández-Cuevas, Elvia Ortiz Ortiz, Edelmira García Nieto, Araceli Tomasini, Edgar Sierra-Palacios, Jaime Marcial-Quino, and et al. 2023. "Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body" International Journal of Molecular Sciences 24, no. 9: 8143. https://doi.org/10.3390/ijms24098143

APA Style

Armas-Tizapantzi, A., Martínez y Pérez, J. L., Fernández, F. J., Mata, G., Hernández-Cuevas, L. V., Ortiz Ortiz, E., García Nieto, E., Tomasini, A., Sierra-Palacios, E., Marcial-Quino, J., & Montiel-González, A. M. (2023). Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body. International Journal of Molecular Sciences, 24(9), 8143. https://doi.org/10.3390/ijms24098143

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