Fractalkine Improves the Expression of Endometrium Receptivity-Related Genes and Proteins at Desferrioxamine-Induced Iron Deficiency in HEC-1A Cells
Abstract
:1. Introduction
2. Results
2.1. Iron Deficiency Significantly Decreases the mRNA Expression Levels of the Endometrium Receptivity-Related Genes
2.2. Fractalkine Ameliorates the Effect of Iron Deficiency on the mRNA Expression of Activin and Follistatin, and the Secretions of FKN and BMP2 of the HEC-1A Cells
2.3. Fractalkine Alters the Effect of Iron Deficiency on the Secreted Cytokines Related to Endometrium Receptivity
2.4. Fractalkine Modifies the Levels of the Endometrium Receptivity-Related Proteins at Iron Deficiency
2.5. The Effect of Fractalkine on the Expression of PTGER2 and TIMP2 Depends on the Iron Availability of HEC-1A Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Real-Time PCR
4.3. Enzyme-Linked Immunosorbent Assay (ELISA) Measurements
4.4. Western Blot Analysis
4.5. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sequence 5′→3′ |
---|---|
activin A forward | GAACTTATGGAGCAGACCTC |
activin A reverse | GGACTTTTAGGAAGAGCCAG |
BMP2 forward | TAAGTTCTATCCCCACGGAG |
BMP2 reverse | AGCATCTTGCATCTGTTCTC |
CX3CR1 forward | CCATTAGTCTGGGCGTCTGG |
CX3CR1 reverse | GTCACCCAGACACTCGTTGT |
FKN forward | TACCTGTAGCTTTGCTCATC |
FKN reverse | GTCTCGTCTCCAAGATGATT |
follistatin forward | CAAAGCAAAGTCCTGTGAAG |
follistatin reverse | CCTCTCCCAACCTTGAAATC |
GAPDH forward | TGTTCCAATATGATTCCACCC |
GAPDH reverse | CCACTTGATTTTGGAGGGAT |
IL-1β forward | GAAATGATGGCTTATTACAGTGG |
IL-1β reverse | GGTGGTCGGAGATTCGTA |
IL-6 forward | CTGAGAAAGGAGACATGTAACAAGA |
IL-6 reverse | GGCAAGTCTCCTCATTGAATC |
IL-8 forward | CAGTGCATAAAGACATACTCC |
IL-8 reverse | CACTCTCAATCACTCTCAGT |
LIF forward | CTCGGGTAAGGATGTCTTC |
LIF reverse | GGCGATGATCTGCTTATACT |
Progesterone receptor A/B forward | CCAAAGGCCGCAAATTCT |
Progesterone receptor A/B reverse | TGAGGTCAGAAAGGTCATCG |
SOX-17 forward | CAGTATCTGCACTTCGTGTG |
SOX-17 reverse | AGTAATATACCGCGGAGCTG |
TGF-β forward | GCCAGAGTGGTTATCTTTTG |
TGF-β reverse | GTAGTGAACCCGTTGATGT |
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Pandur, E.; Pap, R.; Jánosa, G.; Horváth, A.; Sipos, K. Fractalkine Improves the Expression of Endometrium Receptivity-Related Genes and Proteins at Desferrioxamine-Induced Iron Deficiency in HEC-1A Cells. Int. J. Mol. Sci. 2023, 24, 7924. https://doi.org/10.3390/ijms24097924
Pandur E, Pap R, Jánosa G, Horváth A, Sipos K. Fractalkine Improves the Expression of Endometrium Receptivity-Related Genes and Proteins at Desferrioxamine-Induced Iron Deficiency in HEC-1A Cells. International Journal of Molecular Sciences. 2023; 24(9):7924. https://doi.org/10.3390/ijms24097924
Chicago/Turabian StylePandur, Edina, Ramóna Pap, Gergely Jánosa, Adrienn Horváth, and Katalin Sipos. 2023. "Fractalkine Improves the Expression of Endometrium Receptivity-Related Genes and Proteins at Desferrioxamine-Induced Iron Deficiency in HEC-1A Cells" International Journal of Molecular Sciences 24, no. 9: 7924. https://doi.org/10.3390/ijms24097924