Evaluation of Mitochondrial Function in Blood Samples Shows Distinct Patterns in Subjects with Thyroid Carcinoma from Those with Hyperplasia
Abstract
:1. Introduction
2. Results
2.1. Characterization of the Cohort: Basic Metabolic Risk Factors and Histological Analysis
2.2. Characterization of Thyroid Tissue Metabolic Status
2.3. Evaluation of Thyroid DNA in Plasma Samples
2.4. Evaluation of the Systemic Metabolic Status of Patient-Derived PBMCs
2.5. Analysis of the Systemic Inflammatory Status
2.6. Comparison of Systemic and Tumor Metabolism
2.7. Origin and Significance of cfDNA Detectable in Crude Plasma Samples
2.8. Functional Significance of the Observed Changes in ND1 and ND4
3. Discussion
4. Materials and Methods
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
References
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Cohort 1 n = 45 | p | Cohort 2 n = 28 | p | |||
---|---|---|---|---|---|---|
Hyperplasias n = 24 | Carcinomas n = 21 | ns | Hyperplasias n = 16 | Carcinomas n = 12 | ns | |
Male | 3 | 5 | ns | 3 | 2 | ns |
Female | 21 | 16 | ns | 13 | 10 | ns |
Age | 62.43+/−12.87 | 52.76+/−16 | 0.032 | 53.06+/−12.80 | 53.83+/−13.5 | ns |
BMI | 25.72+/−4.49 | 27.27+/−4.40 | ns | 29.67+/−7.37 | 28.76+/−5.10 | ns |
Fasting glucose (mg/dl) | 98.33+/−14.86 | 93.85+/−10.80 | ns | 104.13+/−45.43 | 107.17+/−24.96 | ns |
Cholesterol (mg/dl) | 209.69+/−25.98 | 206.00+/−43.17 | ns | 184.64+/−41.09 | 191.83+/−37.56 | ns |
Triglycerides (mg/dl) | 122.13+/−84.18 | 116.83+/−67.43 | ns | 98.77+/−53.40 | 138.83+/−65.88 | ns |
Smokers | 3 | 7 | ns | 2 | 3 | ns |
Blood pressure (Max. mm Hg) | 137.33+/−18.41 | 135.50+/−15.58 | ns | 141.87+/−23.16 | 127.18+/−28.20 | ns |
Blood pressure (Min. mm Hg) | 78.14+/−10.41 | 79.70+/−10.53 | ns | 81.31+/−15.70 | 79.18+/−11.81 | ns |
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Bernal-Tirapo, J.; Bayo Jiménez, M.T.; Yuste-García, P.; Cordova, I.; Peñas, A.; García-Borda, F.-J.; Quintela, C.; Prieto, I.; Sánchez-Ramos, C.; Ferrero-Herrero, E.; et al. Evaluation of Mitochondrial Function in Blood Samples Shows Distinct Patterns in Subjects with Thyroid Carcinoma from Those with Hyperplasia. Int. J. Mol. Sci. 2023, 24, 6453. https://doi.org/10.3390/ijms24076453
Bernal-Tirapo J, Bayo Jiménez MT, Yuste-García P, Cordova I, Peñas A, García-Borda F-J, Quintela C, Prieto I, Sánchez-Ramos C, Ferrero-Herrero E, et al. Evaluation of Mitochondrial Function in Blood Samples Shows Distinct Patterns in Subjects with Thyroid Carcinoma from Those with Hyperplasia. International Journal of Molecular Sciences. 2023; 24(7):6453. https://doi.org/10.3390/ijms24076453
Chicago/Turabian StyleBernal-Tirapo, Julia, María Teresa Bayo Jiménez, Pedro Yuste-García, Isabel Cordova, Ana Peñas, Francisco-Javier García-Borda, Cesar Quintela, Ignacio Prieto, Cristina Sánchez-Ramos, Eduardo Ferrero-Herrero, and et al. 2023. "Evaluation of Mitochondrial Function in Blood Samples Shows Distinct Patterns in Subjects with Thyroid Carcinoma from Those with Hyperplasia" International Journal of Molecular Sciences 24, no. 7: 6453. https://doi.org/10.3390/ijms24076453