Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol
Abstract
:1. Introduction
2. Results
2.1. HSC Profibrotic Activation after Internalization of Acetaldehyde- and HIV-Derived Hepatocyte ABSS
2.2. Lack of Profibrotic Changes in HSC after Internalization of ABs Derived from HIV- and AGS-Exposed Lymphocytes
2.3. Proteins Expressed in ABsAGS+HIV(RLW) and ABsAGS+HIV(JK) Associated with Hepatotoxicity
2.4. Proteins in Hepatocyte ABS vs. Lymphocyte ABs Associated with Liver Fibrosis
2.5. Suppression of Profibrotic Genes in LX2 Cells after Internalization of ABsAGS+HIV Derived from HDGF-Knockdown RLW Cells
2.6. Alcohol Consumption Exacerbated HIV-1-Induced Immunopathology but Did Not Affect Liver Fibrosis in the Absence of Transplanted Human Hepatocytes
2.7. Alcohol Consumption Increased Human Immune Cells Infiltration but Did Not Induce Fibrotic Changes in the Liver of HIV-1-Infected Humanized Mice
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. In Vitro Studies
4.3. siRNA Transfection of LX2 Cells
4.4. Sample Preparation for Mass Spectrometry
4.5. LC-MS/MS
4.6. Data Analysis and Bioinformatics Analysis
4.7. RNA Isolation and RT-PCR
4.8. Immunoblotting/Western Blotting
4.9. In Vivo Studies
4.9.1. Humanized Mice
4.9.2. Immunohistochemistry
4.9.3. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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New-Aaron, M.; Koganti, S.S.; Ganesan, M.; Kanika, S.; Kumar, V.; Wang, W.; Makarov, E.; Kharbanda, K.K.; Poluektova, L.Y.; Osna, N.A. Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol. Int. J. Mol. Sci. 2023, 24, 5346. https://doi.org/10.3390/ijms24065346
New-Aaron M, Koganti SS, Ganesan M, Kanika S, Kumar V, Wang W, Makarov E, Kharbanda KK, Poluektova LY, Osna NA. Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol. International Journal of Molecular Sciences. 2023; 24(6):5346. https://doi.org/10.3390/ijms24065346
Chicago/Turabian StyleNew-Aaron, Moses, Siva Sankar Koganti, Murali Ganesan, Sharma Kanika, Vikas Kumar, Weimin Wang, Edward Makarov, Kusum K. Kharbanda, Larisa Y. Poluektova, and Natalia A. Osna. 2023. "Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol" International Journal of Molecular Sciences 24, no. 6: 5346. https://doi.org/10.3390/ijms24065346