Recognizing Minor Leukemic Populations with Monocytic Features in Mixed-Phenotype Acute Leukemia by Flow Cell Sorting Followed by Cytogenetic and Molecular Studies: Report of Five Exemplary Cases
Abstract
:1. Introduction
2. Results
2.1. Patient #1
2.2. Patient #2
2.3. Patient #3
2.4. Patient #4
2.5. Patient #5
3. Discussion
4. Materials and Methods
4.1. Patients and Samples
4.2. Morphology and Cytochemistry
4.3. Flow Cytometry
4.4. Flow Cell Sorting
4.5. Fluorescence In Situ Hybridization
4.6. Detection of IG Gene Rearrangements by Multiplex PCR
4.7. Detection of Clonal Rearrangements of IG and TR Genes by Next-Generation Sequencing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Semchenkova, A.; Zerkalenkova, E.; Demina, I.; Kashpor, S.; Volchkov, E.; Zakharova, E.; Larin, S.; Olshanskaya, Y.; Novichkova, G.; Maschan, A.; et al. Recognizing Minor Leukemic Populations with Monocytic Features in Mixed-Phenotype Acute Leukemia by Flow Cell Sorting Followed by Cytogenetic and Molecular Studies: Report of Five Exemplary Cases. Int. J. Mol. Sci. 2023, 24, 5260. https://doi.org/10.3390/ijms24065260
Semchenkova A, Zerkalenkova E, Demina I, Kashpor S, Volchkov E, Zakharova E, Larin S, Olshanskaya Y, Novichkova G, Maschan A, et al. Recognizing Minor Leukemic Populations with Monocytic Features in Mixed-Phenotype Acute Leukemia by Flow Cell Sorting Followed by Cytogenetic and Molecular Studies: Report of Five Exemplary Cases. International Journal of Molecular Sciences. 2023; 24(6):5260. https://doi.org/10.3390/ijms24065260
Chicago/Turabian StyleSemchenkova, Alexandra, Elena Zerkalenkova, Irina Demina, Svetlana Kashpor, Egor Volchkov, Elena Zakharova, Sergey Larin, Yulia Olshanskaya, Galina Novichkova, Alexey Maschan, and et al. 2023. "Recognizing Minor Leukemic Populations with Monocytic Features in Mixed-Phenotype Acute Leukemia by Flow Cell Sorting Followed by Cytogenetic and Molecular Studies: Report of Five Exemplary Cases" International Journal of Molecular Sciences 24, no. 6: 5260. https://doi.org/10.3390/ijms24065260