Aberrantly Expressed MicroRNAs in Cancer-Associated Fibroblasts and Their Target Oncogenic Signatures in Hepatocellular Carcinoma
Abstract
:1. Introduction
2. Results
2.1. Identification of Differentially Expressed miRs in HCC-CAFs
2.2. Functional Analysis of Target Gene Signatures Regulated by miR-101-3P and miR-490-3p
2.3. TGFBR1 Was Identified as a Common Target Gene of miR-101-3p and miR-490-3p
2.4. TGFBR1 Expression Was Negatively Regulated by hsa-miR-101-3p and hsa-miR-490-3p
2.5. Prognostic Implication of TGFBR1 in HCC Patients
2.6. Immunological Implication of TGFBR1 in HCC Patients
3. Discussion
4. Materials and Methods
4.1. Isolation of CAFs, PAFs, and NFs from Surgically Resected Liver Tissue
4.2. Small-RNA-Sequencing
4.3. Gene Expression Profiling Using Public Omics Databases
4.4. Prediction of miR Target Genes
4.5. Gene Ontology Analysis and Molecular Pathway Mining
4.6. Network Analysis between the Hub-Target Genes and CAF-Related miRs
4.7. Survival Analyses
4.8. TGFBR1 Protein Expression in Human HCC tissues
4.9. Cell Culture, Collection of CAF-CM, and miR Mimic Transfection
4.10. RNA Isolation and Quantitative Reverse-Transcription Polymerase Chain Reaction Analysis
4.11. Western Blot Analysis
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Eun, J.W.; Ahn, H.R.; Baek, G.O.; Yoon, M.G.; Son, J.A.; Weon, J.H.; Yoon, J.H.; Kim, H.S.; Han, J.E.; Kim, S.S.; et al. Aberrantly Expressed MicroRNAs in Cancer-Associated Fibroblasts and Their Target Oncogenic Signatures in Hepatocellular Carcinoma. Int. J. Mol. Sci. 2023, 24, 4272. https://doi.org/10.3390/ijms24054272
Eun JW, Ahn HR, Baek GO, Yoon MG, Son JA, Weon JH, Yoon JH, Kim HS, Han JE, Kim SS, et al. Aberrantly Expressed MicroRNAs in Cancer-Associated Fibroblasts and Their Target Oncogenic Signatures in Hepatocellular Carcinoma. International Journal of Molecular Sciences. 2023; 24(5):4272. https://doi.org/10.3390/ijms24054272
Chicago/Turabian StyleEun, Jung Woo, Hye Ri Ahn, Geum Ok Baek, Moon Gyeong Yoon, Ju A Son, Ji Hyang Weon, Jung Hwan Yoon, Hyung Seok Kim, Ji Eun Han, Soon Sun Kim, and et al. 2023. "Aberrantly Expressed MicroRNAs in Cancer-Associated Fibroblasts and Their Target Oncogenic Signatures in Hepatocellular Carcinoma" International Journal of Molecular Sciences 24, no. 5: 4272. https://doi.org/10.3390/ijms24054272