LPS-Activated Microglial Cell-Derived Conditioned Medium Protects HT22 Neuronal Cells against Glutamate-Induced Ferroptosis
Abstract
:1. Introduction
2. Results
2.1. LPS Is Not Cytotoxic and Induces Microglial Activation
2.2. LPS and CM Were Not Cytotoxic in HT22 Cells
2.3. Glutamate and t-BuOOH Induced a Concentration-Dependent Cytotoxicity in HT22 Cells
2.4. CM Derived from LPS-Stimulated Microglial Cells Protected against Glutamate-Induced Ferroptosis, but Not against the Oxidative Toxicity Induced by t-BuOOH in HT22 Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals/Reagents
4.2. Cell Cultures
4.3. Production of Conditioned Media and Nitric Oxide Assay
4.4. Glutamate and t-BuOOH Exposure Paradigm in HT22 Cells
4.5. CM Exposure Paradigm in HT22 Cells
4.6. Cell Metabolic Viability Assay
4.7. Cell Death Assay
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jacques, M.T.; Saso, L.; Farina, M. LPS-Activated Microglial Cell-Derived Conditioned Medium Protects HT22 Neuronal Cells against Glutamate-Induced Ferroptosis. Int. J. Mol. Sci. 2023, 24, 2910. https://doi.org/10.3390/ijms24032910
Jacques MT, Saso L, Farina M. LPS-Activated Microglial Cell-Derived Conditioned Medium Protects HT22 Neuronal Cells against Glutamate-Induced Ferroptosis. International Journal of Molecular Sciences. 2023; 24(3):2910. https://doi.org/10.3390/ijms24032910
Chicago/Turabian StyleJacques, Mauricio Tavares, Luciano Saso, and Marcelo Farina. 2023. "LPS-Activated Microglial Cell-Derived Conditioned Medium Protects HT22 Neuronal Cells against Glutamate-Induced Ferroptosis" International Journal of Molecular Sciences 24, no. 3: 2910. https://doi.org/10.3390/ijms24032910