Characterization and Preliminary Application of a Novel Lytic Vibrio parahaemolyticus Bacteriophage vB_VpaP_SJSY21
Abstract
:1. Introduction
2. Results
2.1. Isolation, Purification, and General Characterization of Phages
2.2. MOI and One-Step Growth
2.3. Results of Lysis and Temperature Stability Assay
2.4. Characteristics of Phage Genome
2.5. Protein Function Prediction of Bacteriophages
2.6. Analysis of α Diversity and β Diversity
2.7. Analysis of Community Composition Structure and Differential Species
3. Discussion
4. Materials and Methods
4.1. Isolation, Identification, and Purification of Phages
4.2. Morphological Characterization
4.3. Determination of Phage Titer
4.4. Determination of Multiplicity of Infection (MOI) and One-Step Growth
4.5. Lysis Assay and Temperature Stability Assay
4.6. Whole-Genome Sequencing and Analysis
4.7. Experimental Animals and Immune Stimulation
4.8. Extraction and Sequencing of Gut Microbiota Genome
4.9. Analysis of Gut Microbiota Community Structure of L. vannamei
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Read Number | Total Bases (bp) | N Rate (%) | GC Content (%) | Q20 Rate (%) | Q30 Rate (%) |
---|---|---|---|---|---|
20,297,674 | 3,044,540,950 | 0.000779 | 40.8 | 98.52 | 94.98 |
Size (bp) | GC Content (%) | A (%) | G (%) | C (%) | T (%) |
---|---|---|---|---|---|
110,776 | 40.91 | 29.48 | 19.37 | 21.54 | 29.61 |
ORF Number | EggNOG Number | Prediction of Protein Functions |
---|---|---|
ORF 1 | ENOG411EP44 | DNA polymerase |
ORF 17 | ENOG411EPEK | PhoH-like protein |
ORF 20 | ENOG411EP1T | Deoxyribonucleoside diphosphate metabolic process |
ORF 21 | ENOG411EPH2 | Ribonucleotide reductase, barrel domain |
ORF 35 | ENOG411EPBV | Flavin adenine dinucleotide binding |
ORF 47 | ENOG411EPAI | NAD biosynthetic process |
ORF 53 | ENOG411EP4J | Macro domain |
ORF 55 | ENOG411EPQT | Magnesium ion binding |
ORF 57 | ENOG411EP64 | DNA helicase |
ORF 71 | ENOG411EP4Y | - |
ORF 73 | ENOG411EPJD | Glutamine amidotransferase domain |
ORF 76 | ENOG411EPAU | Phage phiEco32-like COOH.NH2 ligase-type 2 |
ORF 152 | ENOG411EP64 | DNA helicase |
ORF 153 | ENOG411EPA3 | - |
ORF 156 | ENOG411EPGN | - |
ORF 167 | ENOG411EP2N | - |
ORF 175 | ENOG411EP0H | T4 recombination endonuclease VII |
ORF 185 | ENOG411EP9Z | Exonuclease activity |
ORF 194 | ENOG411EP4C | - |
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Xu, Y.; Sun, J.; Hu, J.; Bao, Z.; Wang, M. Characterization and Preliminary Application of a Novel Lytic Vibrio parahaemolyticus Bacteriophage vB_VpaP_SJSY21. Int. J. Mol. Sci. 2023, 24, 17529. https://doi.org/10.3390/ijms242417529
Xu Y, Sun J, Hu J, Bao Z, Wang M. Characterization and Preliminary Application of a Novel Lytic Vibrio parahaemolyticus Bacteriophage vB_VpaP_SJSY21. International Journal of Molecular Sciences. 2023; 24(24):17529. https://doi.org/10.3390/ijms242417529
Chicago/Turabian StyleXu, Yajin, Jun Sun, Jingjie Hu, Zhenmin Bao, and Mengqiang Wang. 2023. "Characterization and Preliminary Application of a Novel Lytic Vibrio parahaemolyticus Bacteriophage vB_VpaP_SJSY21" International Journal of Molecular Sciences 24, no. 24: 17529. https://doi.org/10.3390/ijms242417529