Impact of a High-Fat Diet at a Young Age on Wound Healing in Mice
Abstract
:1. Introduction
2. Results
2.1. Early High-Fat Diet Induces Obese Phenotype and Delays Normal Wound Healing
2.2. Granulation Tissue Quality and Thickness Impaired with an Early High-Fat Diet
2.3. Delayed Myofibroblast Appearance in HFD-Fed Mice
2.4. Early High-Fat Diet Increases Mediators of Inflammation
2.5. Macrophage Quantification and M1/M2 Phenotype Assessment
2.6. Early Vascularization Is Decreased after Wounding in Early HFD-Fed Animals
3. Discussion
4. Materials and Methods
4.1. Ethical Approval
4.2. Animals
4.3. Diets
4.4. Wounding of Mice
4.5. Real-Time Quantitative PCR (RT-qPCR)
4.6. Histology: Masson–Goldner-Trichrome Staining and Immunohistochemistry
4.7. Granulation Tissue Quality Assessment
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
CD11c | TGC CAG GAT GAC CTT AGT GTC G | CAG AGT GAC TGT GGT TCC GTA G |
CD301b | GAC TGA GTT CTC GCC TCT GG | CTG GGA AGG AAT TAG AGC AAA CT |
Col I | CTG GCG GTT CAG GTC CAA TG | GAA GCC TCG GTG TCC CTT CA |
Col III | GAC CAA AAG GTG ATG CTG GAC AG | CAA GAC CTC GTG CTC CAG TTA G |
Col IV | GGT GTG CGG TTT GTG AAG CA | TGG CGT GGG CTT CTT GAA CA |
Eln | TCC TGG GAT TGG AGG CAT TGC A | ACC AGG CAC TAA ACC TCC AGC A |
Fn | CGG ACG CTG CGA AAA GAT GA | ACT TGG CTG GCA ACC CTT CT |
Il-6 | TAC CAC TTC ACA AGT CGG AGG C | CTG CAA GTG CAT CAT CGT TGT TC |
Mif | CGC TTT GTA CCG TCC T | CGT GCC GCT AAA AGT CA |
Mif-2/D-Dt | CTC TTC TCC CGC TAA CAT GC | TCA TGC CAG GTC GTA TCG TA |
Pdgf A | GCA AGA CCA GGA CGG TCA TTT AC | TGT TCA GGA ATG TCA CAC GCC |
Tgf-β1 | TGA TAC GCC TGA GTG GCT GTC T | CAC AAG AGC AGT GAG CGC TGA A |
Primary Antibody | Host Species | Used Dilution | Secondary Antibody-HRP |
---|---|---|---|
CD31 | rabbit (polyclonal) | 1:50 | anti-rabbit |
F4/80 | rat IgG (monoclonal) | 1:400 | anti-rat IgG |
F(ab) | goat IgG (polyclonal) | 1:50 | - |
α-SMA | mouse (monoclonal) | 1:2 | anti-mouse |
tumor necrosis factor-α | rabbit (monoclonal) | 1:100 | anti-rabbit |
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Arnke, K.; Pfister, P.; Reid, G.; Vasella, M.; Ruhl, T.; Seitz, A.-K.; Lindenblatt, N.; Cinelli, P.; Kim, B.-S. Impact of a High-Fat Diet at a Young Age on Wound Healing in Mice. Int. J. Mol. Sci. 2023, 24, 17299. https://doi.org/10.3390/ijms242417299
Arnke K, Pfister P, Reid G, Vasella M, Ruhl T, Seitz A-K, Lindenblatt N, Cinelli P, Kim B-S. Impact of a High-Fat Diet at a Young Age on Wound Healing in Mice. International Journal of Molecular Sciences. 2023; 24(24):17299. https://doi.org/10.3390/ijms242417299
Chicago/Turabian StyleArnke, Kevin, Pablo Pfister, Gregory Reid, Mauro Vasella, Tim Ruhl, Ann-Kathrin Seitz, Nicole Lindenblatt, Paolo Cinelli, and Bong-Sung Kim. 2023. "Impact of a High-Fat Diet at a Young Age on Wound Healing in Mice" International Journal of Molecular Sciences 24, no. 24: 17299. https://doi.org/10.3390/ijms242417299