Olanzapine Effects on Parvalbumin/GAD67 Cell Numbers in Layers/Subregions of Dorsal Hippocampus of Chronically Socially Isolated Rats
Abstract
:1. Introduction
2. Results
2.1. Behavior Testing—Forced Swim Test
2.2. Layer-Specific Distribution of the Number of PV+ and GAD67+ Cells in the dCA1 Subregion
2.3. Layer-Specific Distribution of the Number of PV+ and GAD67+ Cells in the CA2 Subregion
2.4. Layer-Specific Distribution of the Number of PV+ and GAD67+ Cells in the dCA3 Subregion
2.5. The Number of PV+ and GAD67+ Cells in the ML-GCL and H of the dDG Subregion
2.6. The Number of PV+ and GAD67+ Cells in dCA1, CA2, dCA3, and dDG Subregions
2.7. Western Blot Analyses
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Olanzapine Solution
4.3. Study Design
4.4. Behavioral Test
Forced Swim Test
4.5. Immunofluorescence
4.6. Preparation of Cytosolic Fractions from the Rat Hippocampus
4.7. Western Blot Analysis
4.8. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Forced Swim Test | |||||||||
---|---|---|---|---|---|---|---|---|---|
Immobility Time (Seconds) | Swimming Time (Seconds) | Climbing Time (Seconds) | |||||||
Group | 0 week | 3rd weeks | 6th weeks | 0 week | 3rd weeks | 6th weeks | 0 week | 3rd weeks | 6th weeks |
Cont | 78 ± 13 | 43 ± 11 ** | |||||||
CSIS | 57 ± 21 | 137 ± 37 ** | 169 ± 26 *** | 182 ± 7 | 124 ± 27 * | 109 ± 17 * | 77 ± 17 | 39 ± 17 ** | 22 ± 11 *** |
CSIS + Olz | 85 ± 17 ## | 186 ± 9 ## |
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Stanisavljević Ilić, A.; Đorđević, S.; Inta, D.; Borgwardt, S.; Filipović, D. Olanzapine Effects on Parvalbumin/GAD67 Cell Numbers in Layers/Subregions of Dorsal Hippocampus of Chronically Socially Isolated Rats. Int. J. Mol. Sci. 2023, 24, 17181. https://doi.org/10.3390/ijms242417181
Stanisavljević Ilić A, Đorđević S, Inta D, Borgwardt S, Filipović D. Olanzapine Effects on Parvalbumin/GAD67 Cell Numbers in Layers/Subregions of Dorsal Hippocampus of Chronically Socially Isolated Rats. International Journal of Molecular Sciences. 2023; 24(24):17181. https://doi.org/10.3390/ijms242417181
Chicago/Turabian StyleStanisavljević Ilić, Andrijana, Snežana Đorđević, Dragoš Inta, Stefan Borgwardt, and Dragana Filipović. 2023. "Olanzapine Effects on Parvalbumin/GAD67 Cell Numbers in Layers/Subregions of Dorsal Hippocampus of Chronically Socially Isolated Rats" International Journal of Molecular Sciences 24, no. 24: 17181. https://doi.org/10.3390/ijms242417181