Two High-Quality Cygnus Genome Assemblies Reveal Genomic Variations Associated with Plumage Color
Abstract
:1. Introduction
2. Results
2.1. Genome Sequencing, Assembly and Annotation
2.2. Phylogenetic Analysis
2.3. Unique and Expanded Orthologue Groups
2.4. Structural Variants
2.5. Positive Selection
2.6. Genes with Amino Acid Replacements
2.7. Relationship between PWWP2A Mutation and Melanin Deposition in Zebrafish
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Genomic Sequencing
4.2. Genome Size Estimation
4.3. Genomic Assembly and Evaluation
4.4. Genome Annotation
4.5. Comparative Phylogenomics
4.6. Whole-Mount In Situ Hybridization (WISH)
4.7. Generation of Mutant Fish
4.8. RT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Contig Number | Contig Length (Mb) | Contig N50 (Mb) | Contig N90 (Mb) | Contig Max (Mb) | GC Content (%) | Complete BUSCOs |
---|---|---|---|---|---|---|---|
C. olor | 335 | 1124.00 | 26.82 | 4.90 | 77.28 | 42.04 | 2546 (98.45%) |
C. atratus | 469 | 1129.04 | 21.91 | 4.55 | 65.49 | 42.06 | 2542 (98.30%) |
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Chong, Y.; Tu, X.; Lu, Y.; Gao, Z.; He, X.; Hong, J.; Wu, J.; Wu, D.; Xi, D.; Deng, W. Two High-Quality Cygnus Genome Assemblies Reveal Genomic Variations Associated with Plumage Color. Int. J. Mol. Sci. 2023, 24, 16953. https://doi.org/10.3390/ijms242316953
Chong Y, Tu X, Lu Y, Gao Z, He X, Hong J, Wu J, Wu D, Xi D, Deng W. Two High-Quality Cygnus Genome Assemblies Reveal Genomic Variations Associated with Plumage Color. International Journal of Molecular Sciences. 2023; 24(23):16953. https://doi.org/10.3390/ijms242316953
Chicago/Turabian StyleChong, Yuqing, Xiaolong Tu, Ying Lu, Zhendong Gao, Xiaoming He, Jieyun Hong, Jiao Wu, Dongdong Wu, Dongmei Xi, and Weidong Deng. 2023. "Two High-Quality Cygnus Genome Assemblies Reveal Genomic Variations Associated with Plumage Color" International Journal of Molecular Sciences 24, no. 23: 16953. https://doi.org/10.3390/ijms242316953