Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Decellularization and pcECM Characterization
4.2. hMSC Culture and Characterization
4.3. pcECM-Induced MET in hMSCs
4.4. Bioinformatic Analyses
4.5. CRISPR-Cas9 Knockout of HAND2-HAND2-AS1 in hMSCs
4.6. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vazana-Netzarim, R.; Elmalem, Y.; Sofer, S.; Bruck, H.; Danino, N.; Sarig, U. Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells. Int. J. Mol. Sci. 2023, 24, 16546. https://doi.org/10.3390/ijms242216546
Vazana-Netzarim R, Elmalem Y, Sofer S, Bruck H, Danino N, Sarig U. Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2023; 24(22):16546. https://doi.org/10.3390/ijms242216546
Chicago/Turabian StyleVazana-Netzarim, Rachel, Yishay Elmalem, Shachar Sofer, Hod Bruck, Naama Danino, and Udi Sarig. 2023. "Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells" International Journal of Molecular Sciences 24, no. 22: 16546. https://doi.org/10.3390/ijms242216546