Keratinocytes Exposed to Blue or Red Light: Proteomic Characterization Showed Cytoplasmic Thioredoxin Reductase 1 and Aldo-Keto Reductase Family 1 Member C3 Triggered Expression
Abstract
:1. Introduction
2. Results
2.1. High-Irradiance Blue or Red Light Reduced HaCaT Cell Viability
2.2. Apoptotic Effects, Cell Cycle Dysfunction and Alteration in Circadian Clock Gene Expression of High-Irradiance Blue Light Exposure in HaCaT Cells
2.3. Proteomic Profile Revealed a Role of Oxidative Stress Response-Related Proteins Deregulated in HaCaT Cells Exposed to High-Irradiance Blue Light
2.4. High-Irradiance Blue Light Upregulated AKR1C3 and TXNRD1 Expression Levels while Blue or Red Light Exposure Increased the SOD1 Protein Expression Level
2.5. High-Irradiance Blue Light Increased ROS Production Level and Correlated with Higher Mitochondrial Density in HaCaT Cells
2.6. Effect of High-Irradiance Blue or Red Light Exposure on the Cell Structure of HaCaT Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Light Exposure
4.3. Cell Viability Assay
4.4. Cell Density Assay
4.5. Apoptosis Assay
4.6. Cell Cycle Analysis
4.7. Total RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
4.8. Protein Extraction and Digestion for MS Analysis
4.9. Mass Spectrometry Analysis
4.10. Bioinformatics Analysis
4.11. Protein Extraction and Western Blotting
4.12. Intracellular ROS Detection
4.13. Mitotracker Green Staining
4.14. Transmission Electron Microscope (TEM)
4.15. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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16 COMMON PROTEINS DOWNREGULATED | 21 COMMON PROTEINS UPREGULATED |
---|---|
AGRN | AHCYL1 |
COPZ1 | AKR1C2 |
CTSB | AKR1C3 |
DERA | CAST |
EVPL | DCAF13 |
GLG1 | GNL2 |
IVL | GYS1 |
KDM1A | HDHD3 |
KIAA1522 | LARP4 |
LANCL2 | LSM14A |
NUDT2 | LSM3 |
PKP1 | MPHOSPH10 |
PPP2R5A | MRPS14 |
RUNX1 | |
SNCG | TM9SF3 |
SPAG7 | TMEM43 |
VSNL1 | TRAF2 |
TXNRD1 | |
VPS33A | |
ZNF638 | |
ZW10 |
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Lazzarini, R.; Tartaglione, M.F.; Ciarapica, V.; Piva, F.; Giulietti, M.; Fulgenzi, G.; Martelli, M.; Ledda, C.; Vitale, E.; Malavolta, M.; et al. Keratinocytes Exposed to Blue or Red Light: Proteomic Characterization Showed Cytoplasmic Thioredoxin Reductase 1 and Aldo-Keto Reductase Family 1 Member C3 Triggered Expression. Int. J. Mol. Sci. 2023, 24, 16189. https://doi.org/10.3390/ijms242216189
Lazzarini R, Tartaglione MF, Ciarapica V, Piva F, Giulietti M, Fulgenzi G, Martelli M, Ledda C, Vitale E, Malavolta M, et al. Keratinocytes Exposed to Blue or Red Light: Proteomic Characterization Showed Cytoplasmic Thioredoxin Reductase 1 and Aldo-Keto Reductase Family 1 Member C3 Triggered Expression. International Journal of Molecular Sciences. 2023; 24(22):16189. https://doi.org/10.3390/ijms242216189
Chicago/Turabian StyleLazzarini, Raffaella, Maria Fiorella Tartaglione, Veronica Ciarapica, Francesco Piva, Matteo Giulietti, Gianluca Fulgenzi, Margherita Martelli, Caterina Ledda, Ermanno Vitale, Marco Malavolta, and et al. 2023. "Keratinocytes Exposed to Blue or Red Light: Proteomic Characterization Showed Cytoplasmic Thioredoxin Reductase 1 and Aldo-Keto Reductase Family 1 Member C3 Triggered Expression" International Journal of Molecular Sciences 24, no. 22: 16189. https://doi.org/10.3390/ijms242216189