Genetic Enhancement for Biotic Stress Resistance in Basmati Rice through Marker-Assisted Backcross Breeding
, Alexander Balamurugan 2, G. Prakash 2, Rajeev Rathour 3, Kalyan Kumar Mondal 2, Prolay Kumar Bhowmick 1, S. Gopala Krishnan 1, Mariappan Nagarajan 4, Rakesh Seth 5, K. K. Vinod 1, Varsha Singh 1, Haritha Bollinedi 1 and Ashok Kumar Singh 1,*
Round 1
Reviewer 1 Report
Research manuscript by Singh et al. describes the incorporation of disease tolerance alleles (bacterial blight- xa13, Xa21, Xa38; and fungal blast- Pi9, 101 Pib, Pita) into the genetic background of well-established Basmati rice variety PB1509 through marker-assisted backcross breeding (MABB). The manuscript is well-written, and the data is clear in the provided figures and Tables. This MS can be considered for publication with some changes.
Here are some suggestions:
1. Line 17-18 - bacterial blight resistance genes….; and blast resistance genes…
- Revise as-fungal blast resistance genes…
2. Line 22- SSR- write the full form of abbreviated terms while mentioning it for the first time.
3. Line 23- NILs- write the full form of abbreviated terms while mentioning it for the first time.
4. Line 42- a gram-negative bacteria…
- Revise- a gram-negative bacterium…
5. Line 40 and 44- Check the font size of reference numbers.
6. Lines 57, 65, and many other places– R genes- R should be in italics.
7. Line 60 – Xa8, xa13, Xa21 and Xa38 - and should not be in italic.
8. Line 105 – DP Full form- Donor parents? Write the full form of abbreviated terms while mentioning them for the first time.
9. Line 106 - RP Full form- recurrent parent?? Write the full form of abbreviated terms while mentioning them for the first time.
10. Line 112- RPG, full form. Write the full form of abbreviated terms while mentioning them for the first time.
11. Line 168 – RPGA full form. Write the full form of abbreviated terms while mentioning them for the first time.
12. Line 150 - DF full form. Write the full form of abbreviated terms while mentioning it for the first time.
13. Lines 161, 164, 165, 218 – HR, MR, KLBC, AEC- full forms. Write the full form of abbreviated terms while mentioning them for the first time.
14. Line 222 – Figure 3 – GGE – full form.
15. Table 5 - Write the full forms of all the abbreviations used in the table, DF, PH, ...
16. Line 245 - bacterial blight – abbreviate it to BB.
17. Figure 6 - X-axis title is missing.
18. Line 307 - Pusa 6B etc.,[22,46,47]. – Remove commas.
19. References – Numbers are mentioned twice in the whole list. Remove the duplicated numbers from all the references.
The language is clear.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
necessary suggestions are attached
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
