Improving the Efficacy of Magnetic Nanoparticle-Mediated Hyperthermia Using Trapezoidal Pulsed Electromagnetic Fields as an In Vitro Anticancer Treatment in Melanoma and Glioblastoma Multiforme Cell Lines
Abstract
:1. Introduction
2. Results
2.1. In Vitro Cytotoxicity of APS-SPION
2.2. Safety of the AMF Applicator
2.3. Cell Uptake of MNPs
2.4. Evaluation of the In Vitro Magnetic Hyperthermia Treatment
3. Discussion
4. Materials and Methods
4.1. Experimental Device
4.2. Magnetic Nanoparticles
4.3. Cancer Cell Line
4.4. Cell Culture
4.5. Experimental Protocol of In Vitro Magnetic Hyperthermia
4.6. Post-Magnetic Hyperthermia Calcein-AM/PI Staining
4.7. Cytotoxicity Studies
4.8. Statistical Analysis
4.9. Cellular Localization of MNPs
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Souiade, L.; Domingo-Diez, J.; Alcaide, C.; Gámez, B.; Gámez, L.; Ramos, M.; Serrano Olmedo, J.J. Improving the Efficacy of Magnetic Nanoparticle-Mediated Hyperthermia Using Trapezoidal Pulsed Electromagnetic Fields as an In Vitro Anticancer Treatment in Melanoma and Glioblastoma Multiforme Cell Lines. Int. J. Mol. Sci. 2023, 24, 15933. https://doi.org/10.3390/ijms242115933
Souiade L, Domingo-Diez J, Alcaide C, Gámez B, Gámez L, Ramos M, Serrano Olmedo JJ. Improving the Efficacy of Magnetic Nanoparticle-Mediated Hyperthermia Using Trapezoidal Pulsed Electromagnetic Fields as an In Vitro Anticancer Treatment in Melanoma and Glioblastoma Multiforme Cell Lines. International Journal of Molecular Sciences. 2023; 24(21):15933. https://doi.org/10.3390/ijms242115933
Chicago/Turabian StyleSouiade, Lilia, Javier Domingo-Diez, Cesar Alcaide, Berta Gámez, Linarejos Gámez, Milagros Ramos, and José Javier Serrano Olmedo. 2023. "Improving the Efficacy of Magnetic Nanoparticle-Mediated Hyperthermia Using Trapezoidal Pulsed Electromagnetic Fields as an In Vitro Anticancer Treatment in Melanoma and Glioblastoma Multiforme Cell Lines" International Journal of Molecular Sciences 24, no. 21: 15933. https://doi.org/10.3390/ijms242115933