Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis
Abstract
:1. Introduction
2. Results
2.1. Cucurbitacin I Exerts Antitumorigenic Effects on Breast and Colorectal Cancer Cells
2.2. Induction of M2 Polarization in Macrophages by Coculturing with Cancer Cells and Hypoxia
2.3. Cucurbitacin I Inhibits M2-Like Polarization of Macrophages
2.4. Hmox1 Was Significantly Downregulated in M2-Like Macrophage under Cucurbitacin I
2.5. Hemin Restored the Downregulation of Hmox1 in Macrophages Caused by Cucurbitacin I and the Inhibition of Tumor Cell Migration
3. Discussion
4. Materials and Methods
4.1. Data Source
4.2. RNA-seq Analysis
4.3. Differential Expression Analysis
4.4. Functional Enrichment Analysis
4.5. Single-Cell RNA-Sequencing (scRNA-seq) Analysis
4.6. Reagents and Antibodies
4.7. Cell Lines and Cell Culture
4.8. Co-Culture Procedures
4.9. Cell Counting Kit-8 (CCK-8) Assay
4.10. Scratch Wound-Healing Migration Assay
4.11. Colony Formation Assay
4.12. Quantitative Real-Time PCR Analysis (RT-qPCR)
4.13. Western Blotting (WB) Analysis
4.14. Flow Cytometry Analysis
4.15. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gong, X.; Liu, Y.; Liang, K.; Chen, Z.; Ding, K.; Qiu, L.; Wei, J.; Du, H. Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis. Int. J. Mol. Sci. 2023, 24, 15920. https://doi.org/10.3390/ijms242115920
Gong X, Liu Y, Liang K, Chen Z, Ding K, Qiu L, Wei J, Du H. Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis. International Journal of Molecular Sciences. 2023; 24(21):15920. https://doi.org/10.3390/ijms242115920
Chicago/Turabian StyleGong, Xiaocheng, Yunfei Liu, Keying Liang, Zixi Chen, Ke Ding, Li Qiu, Jinfen Wei, and Hongli Du. 2023. "Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis" International Journal of Molecular Sciences 24, no. 21: 15920. https://doi.org/10.3390/ijms242115920