Molecular Mechanism for Hepatic Glycerolipid Partitioning of n-6/n-3 Fatty Acid Ratio in an Obese Animal Biomodels

Round 1

Reviewer 1 Report

The manuscript entitled “Molecular mechanism for hepatic glycerolipid partitioning of n-6/n-3 fatty acid ration in an obese animal biomodels” is very interesting. The authors investigated the effect of n-6/n-3 ration on hepatic biodistribution of glycerolipid in an obese animal biomodel. They concluded that dietary n-6/n-3 ration of 4:1 has an anti-obesity effect by controlling hepatic distribution through oxidation and esterification of glycerolipid in an obese animal biomodel. However, there are still some errors in this manuscript need to be corrected.

 

1.     The authors made a mistake in figure 1, fatty acids are biosynthesized from Acyl-CoA, the right one should be

                  Acyl-CoA

Fatty acid Fatty acyl-CoA   / 

                    synthetase     

 

Acyl-CoA   Fatty acid

 

                 

2.     The concentration of LPS should be listed.

3.     “accumulation” in line 100 should be “Accumulation”.

4.     14CO2/[14C] in table 3 should be ¹⁴CO₂/[¹⁴C].

5.     ³H] in line 167 should be [³H].

6.     “[¹⁴C]-TAGin” in line 177 should be “[¹⁴C]-TAG in”.

Author Response

Please see the attachment

Author Response File: Author Response.docx

Reviewer 2 Report

The manuscript entitled: Molecular mechanism for hepatic glycerolipid partitioning of 2

n-6/n-3 fatty acid ratio in an obese animal biomodels describes the impact of lower n6/n3 ratios of fatty acids in improvement of obesity in an in vivo model of HFD-fed rats by decreasing hepatic triglycerides and mobilisation of fat as well as increased fatty acid oxidation  

Ø  The abstract:

1.      The abstract should describe in depth more clarification of used methods and obtained results and draw a clear conclusion.

2.      English writing must to be improved, avoid repetition of words by using synonyms, spelling mistakes need to be corrected.

 Ø  The introduction

1.      The authors should have addressed the role of n6 and n3 in pathogenesis of obesity and their impact on cellular signaling pathways involved like:  NF-kB, PPAR and SREBP-1c and their impact on the development of obesity.

Ø  Material and methods

1.      The High fat diet composition be explained in details or cited if used previously.

2.      The authors choose 42 days for obesity induction please cite a reference or explain if a pilot experiment was performed.

3.      The protocol of selected n6/n3 ratios should be cited.

4.      The grouping of rats and number in each group should be well described.

5.      The statistical analysis should be well described in view of the version, place of the software, type of statistical tests used, number of rats per assay and should be specified in table or figure legends. 

Ø  Results

1.      Please include the table number in text.

2.      Figure 1 could be improved by using 2 columns instead of the vertical arrangement.

Ø  Discussion

1.      Should have again address more about the n6/n3 ratio role in obesity.

2.      The author should explain more clearly about their relevant past work and what they have previously reported; although cited (ref., 11) but it would better if they correlate earlier and current work.

3.      The summary needs to be rephrased to address comprehensive summary of the study.

Overall manuscript needs to be English revised and checked for mistyping mistakes

 

 

Author Response

Please see the attachment

Author Response File: Author Response.docx