The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application
Abstract
:1. Introduction
2. Results
2.1. Harvesting of Osteochondral Grafts
2.2. Metabolic Activity of Chondrocytes
2.3. Expression of Anabolic and Catabolic Genes
2.4. Live/Dead Staining
2.5. Histology
2.6. Coefficient of Friction (COF)
2.7. sGAG in the Supernatant
3. Discussion
4. Materials and Methods
4.1. Specimen Preparation and Study Design
4.2. Metabolic Activity
4.3. RNA Isolation
4.4. Gene Expression Analysis
4.5. Histology
4.6. Live/Dead Staining
4.7. Biotribological Test System
4.8. Sulfated Glycosaminoglycans (sGAGs)
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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No. of Condition | Medium Composition |
---|---|
1 | Growth Medium |
2 | Growth Medium + Cytokines |
3 | Growth Medium + Cytokines + 10% GC [4.5 mg/mL] |
4 | Growth Medium + Cytokines + 10% HA [22 mg/mL] |
5 | Growth Medium + Cytokines + 10% GC/HA [4.5 mg/mL and 22 mg/mL] |
Primer | Abreviation | Sequence (3′-5′) |
---|---|---|
Glyceraldehyde-3-phophate dehydrogenase | GAPDH | |
Forward | ATGTTCCAGTATGATTCCACCC | |
Probe | AGCTTCCCGTTCTCTGCCTTGAC | |
Reverse | ATACTCAGCACCAGCATCAC | |
Aggrecan core protein I | ACAN | |
Forward | ACCTACGATGTCTACTGCTACG | |
Probe | AGAAGGTGAACTGCTCCAGGCG | |
Reverse | AGAGTGGCGTTTTGGGATTC | |
Collagen type II, alpha I | COL2A1 | |
Forward | GTGCAACTGGTCCTCTGG | |
Probe | CCTTGTTCGCCTTTGAAGCCAGC | |
Reverse | ACCTCTTTTCCCTTCTTCACC | |
Matrix metalloproteinase 1 | MMP1 | |
Forward | TTCAACCAGGTGCAGGTATC | |
Probe | AAATTCATGCGCTGCCACCCG | |
Reverse | AGCCCCAATGTCA | |
Matrix metalloproteinase 13 | MMP13 | |
Forward | CTAAACATCCCAAAACGCCAG | |
Probe | CCCTTGATGCCATAACCAGTCTCCG | |
Reverse | ACAGCTCTGCTTCAACCT |
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Bauer, C.; Moser, L.B.; Kern, D.; Jeyakumar, V.; Nehrer, S. The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application. Int. J. Mol. Sci. 2023, 24, 14338. https://doi.org/10.3390/ijms241814338
Bauer C, Moser LB, Kern D, Jeyakumar V, Nehrer S. The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application. International Journal of Molecular Sciences. 2023; 24(18):14338. https://doi.org/10.3390/ijms241814338
Chicago/Turabian StyleBauer, Christoph, Lukas B. Moser, Daniela Kern, Vivek Jeyakumar, and Stefan Nehrer. 2023. "The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application" International Journal of Molecular Sciences 24, no. 18: 14338. https://doi.org/10.3390/ijms241814338